Basolateral LPS inhibits NHE3 and HCO 3 - absorption through TLR4/MyD88- dependent ERK activation in medullary thick ascending limb

Bruns Watts, Thampi George, Edward R. Sherwood, David Good

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24 Citations (Scopus)

Abstract

Sepsis is associated with defects in renal tubule function, but the underlying mechanisms are incompletely understood. Recently, we demonstrated that Gramnegative bacterial lipopolysaccharide (LPS) inhibits HCO 3 - absorption in the medullary thick ascending limb (MTAL) through activation of Toll-like receptor 4 (TLR4). Here, we examined the mechanisms responsible for inhibition of HCO 3 - absorption by basolateral LPS. Adding LPS to the bath decreased HCO 3 - absorption by 30% in rat and mouse MTALs perfused in vitro. The inhibition of HCO 3 - absorption was eliminated by the mitogen-activated protein kinase/ extracellular signal-regulated kinase (MEK)/ERK inhibitors U0126 and PD98059. LPS induced a rapid (<15 min) and sustained (up to 60 min) increase in ERK phosphorylation in microdissected MTALs that was blocked by PD98059. The effects of basolateral LPS to activate ERK and inhibit HCO 3 - absorption were eliminated in MTALs from TLR4 -/- and myeloid differentiation factor 88 (MyD88) -/- mice but were preserved in MTALs from TIR (Toll/interleukin-1 receptor) domain-containing adapter-inducing interferon-β (Trif) -/- mice. Basolateral LPS decreased apical Na +/H + exchanger 3 NHE3 activity through a decrease in maximal velocity (V max). The inhibition of NHE3 by LPS was eliminated by MEK/ERK inhibitors. LPS inhibited HCO 3 - absorption despite the presence of physiological stimuli that activate ERK in the MTAL. We conclude that basolateral LPS inhibits HCO 3 - absorption in the MTAL through activation of a TLR4/ MyD88/MEK/ERK pathway coupled to inhibition of NHE3. These studies identify NHE3 as a target of TLR4 signaling in the MTAL and show that bacterial molecules can impair the absorptive functions of renal tubules through inhibition of this exchanger. The ERK pathway links TLR4 to downstream modulation of ion transport proteins and represents a potential target for treatment of sepsis-induced renal tubule dysfunction.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume301
Issue number6
DOIs
StatePublished - Dec 2011

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Myeloid Differentiation Factor 88
Toll-Like Receptor 4
Lipopolysaccharides
Extremities
MAP Kinase Signaling System
Mitogen-Activated Protein Kinase Kinases
Kidney
Sepsis
Sodium-Hydrogen Antiporter
Interleukin-1 Receptors
Extracellular Signal-Regulated MAP Kinases
Ion Transport
Mitogen-Activated Protein Kinases
Baths
Interferons
Carrier Proteins
Phosphorylation

Keywords

  • Acid-base balance
  • Kidney
  • Na /H exchange
  • Sepsis
  • Toll-like receptor 4 signaling

ASJC Scopus subject areas

  • Cell Biology
  • Physiology

Cite this

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title = "Basolateral LPS inhibits NHE3 and HCO 3 - absorption through TLR4/MyD88- dependent ERK activation in medullary thick ascending limb",
abstract = "Sepsis is associated with defects in renal tubule function, but the underlying mechanisms are incompletely understood. Recently, we demonstrated that Gramnegative bacterial lipopolysaccharide (LPS) inhibits HCO 3 - absorption in the medullary thick ascending limb (MTAL) through activation of Toll-like receptor 4 (TLR4). Here, we examined the mechanisms responsible for inhibition of HCO 3 - absorption by basolateral LPS. Adding LPS to the bath decreased HCO 3 - absorption by 30{\%} in rat and mouse MTALs perfused in vitro. The inhibition of HCO 3 - absorption was eliminated by the mitogen-activated protein kinase/ extracellular signal-regulated kinase (MEK)/ERK inhibitors U0126 and PD98059. LPS induced a rapid (<15 min) and sustained (up to 60 min) increase in ERK phosphorylation in microdissected MTALs that was blocked by PD98059. The effects of basolateral LPS to activate ERK and inhibit HCO 3 - absorption were eliminated in MTALs from TLR4 -/- and myeloid differentiation factor 88 (MyD88) -/- mice but were preserved in MTALs from TIR (Toll/interleukin-1 receptor) domain-containing adapter-inducing interferon-β (Trif) -/- mice. Basolateral LPS decreased apical Na +/H + exchanger 3 NHE3 activity through a decrease in maximal velocity (V max). The inhibition of NHE3 by LPS was eliminated by MEK/ERK inhibitors. LPS inhibited HCO 3 - absorption despite the presence of physiological stimuli that activate ERK in the MTAL. We conclude that basolateral LPS inhibits HCO 3 - absorption in the MTAL through activation of a TLR4/ MyD88/MEK/ERK pathway coupled to inhibition of NHE3. These studies identify NHE3 as a target of TLR4 signaling in the MTAL and show that bacterial molecules can impair the absorptive functions of renal tubules through inhibition of this exchanger. The ERK pathway links TLR4 to downstream modulation of ion transport proteins and represents a potential target for treatment of sepsis-induced renal tubule dysfunction.",
keywords = "Acid-base balance, Kidney, Na /H exchange, Sepsis, Toll-like receptor 4 signaling",
author = "Bruns Watts and Thampi George and Sherwood, {Edward R.} and David Good",
year = "2011",
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language = "English (US)",
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T1 - Basolateral LPS inhibits NHE3 and HCO 3 - absorption through TLR4/MyD88- dependent ERK activation in medullary thick ascending limb

AU - Watts, Bruns

AU - George, Thampi

AU - Sherwood, Edward R.

AU - Good, David

PY - 2011/12

Y1 - 2011/12

N2 - Sepsis is associated with defects in renal tubule function, but the underlying mechanisms are incompletely understood. Recently, we demonstrated that Gramnegative bacterial lipopolysaccharide (LPS) inhibits HCO 3 - absorption in the medullary thick ascending limb (MTAL) through activation of Toll-like receptor 4 (TLR4). Here, we examined the mechanisms responsible for inhibition of HCO 3 - absorption by basolateral LPS. Adding LPS to the bath decreased HCO 3 - absorption by 30% in rat and mouse MTALs perfused in vitro. The inhibition of HCO 3 - absorption was eliminated by the mitogen-activated protein kinase/ extracellular signal-regulated kinase (MEK)/ERK inhibitors U0126 and PD98059. LPS induced a rapid (<15 min) and sustained (up to 60 min) increase in ERK phosphorylation in microdissected MTALs that was blocked by PD98059. The effects of basolateral LPS to activate ERK and inhibit HCO 3 - absorption were eliminated in MTALs from TLR4 -/- and myeloid differentiation factor 88 (MyD88) -/- mice but were preserved in MTALs from TIR (Toll/interleukin-1 receptor) domain-containing adapter-inducing interferon-β (Trif) -/- mice. Basolateral LPS decreased apical Na +/H + exchanger 3 NHE3 activity through a decrease in maximal velocity (V max). The inhibition of NHE3 by LPS was eliminated by MEK/ERK inhibitors. LPS inhibited HCO 3 - absorption despite the presence of physiological stimuli that activate ERK in the MTAL. We conclude that basolateral LPS inhibits HCO 3 - absorption in the MTAL through activation of a TLR4/ MyD88/MEK/ERK pathway coupled to inhibition of NHE3. These studies identify NHE3 as a target of TLR4 signaling in the MTAL and show that bacterial molecules can impair the absorptive functions of renal tubules through inhibition of this exchanger. The ERK pathway links TLR4 to downstream modulation of ion transport proteins and represents a potential target for treatment of sepsis-induced renal tubule dysfunction.

AB - Sepsis is associated with defects in renal tubule function, but the underlying mechanisms are incompletely understood. Recently, we demonstrated that Gramnegative bacterial lipopolysaccharide (LPS) inhibits HCO 3 - absorption in the medullary thick ascending limb (MTAL) through activation of Toll-like receptor 4 (TLR4). Here, we examined the mechanisms responsible for inhibition of HCO 3 - absorption by basolateral LPS. Adding LPS to the bath decreased HCO 3 - absorption by 30% in rat and mouse MTALs perfused in vitro. The inhibition of HCO 3 - absorption was eliminated by the mitogen-activated protein kinase/ extracellular signal-regulated kinase (MEK)/ERK inhibitors U0126 and PD98059. LPS induced a rapid (<15 min) and sustained (up to 60 min) increase in ERK phosphorylation in microdissected MTALs that was blocked by PD98059. The effects of basolateral LPS to activate ERK and inhibit HCO 3 - absorption were eliminated in MTALs from TLR4 -/- and myeloid differentiation factor 88 (MyD88) -/- mice but were preserved in MTALs from TIR (Toll/interleukin-1 receptor) domain-containing adapter-inducing interferon-β (Trif) -/- mice. Basolateral LPS decreased apical Na +/H + exchanger 3 NHE3 activity through a decrease in maximal velocity (V max). The inhibition of NHE3 by LPS was eliminated by MEK/ERK inhibitors. LPS inhibited HCO 3 - absorption despite the presence of physiological stimuli that activate ERK in the MTAL. We conclude that basolateral LPS inhibits HCO 3 - absorption in the MTAL through activation of a TLR4/ MyD88/MEK/ERK pathway coupled to inhibition of NHE3. These studies identify NHE3 as a target of TLR4 signaling in the MTAL and show that bacterial molecules can impair the absorptive functions of renal tubules through inhibition of this exchanger. The ERK pathway links TLR4 to downstream modulation of ion transport proteins and represents a potential target for treatment of sepsis-induced renal tubule dysfunction.

KW - Acid-base balance

KW - Kidney

KW - Na /H exchange

KW - Sepsis

KW - Toll-like receptor 4 signaling

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JO - American Journal of Physiology - Endocrinology and Metabolism

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