BCG administration promotes the long-term protection afforded by a single-dose intranasal adenovirus-based SARS-CoV-2 vaccine

Dilhan J. Perera, Pilar Domenech, George Giorgi Babuadze, Maedeh Naghibosadat, Fernando Alvarez, Cal Koger-Pease, Lydia Labrie, Matthew Stuible, Yves Durocher, Ciriaco A. Piccirillo, André Lametti, Pierre Olivier Fiset, Seyyed Mehdy Elahi, Gary P. Kobinger, Rénald Gilbert, Martin Olivier, Robert Kozak, Michael B. Reed, Momar Ndao

Research output: Contribution to journalArticlepeer-review

Abstract

Recent publications have explored intranasal (i.n.) adenovirus-based (Ad) vaccines as an effective strategy for SARS-CoV-2 in pre-clinical models. However, the effects of prior immunizations and infections have yet to be considered. Here, we investigate the immunomodulatory effects of Mycobacterium bovis BCG pre-immunization followed by vaccination with an S-protein-expressing i.n. Ad, termed Ad(Spike). While i.n. Ad(Spike) retains some protective effect after 6 months, a single administration of BCG-Danish prior to Ad(Spike) potentiates its ability to control viral replication of the B.1.351 SARS-CoV-2 variant within the respiratory tract. Though BCG-Danish did not affect Ad(Spike)-generated humoral immunity, it promoted the generation of cytotoxic/Th1 responses over suppressive FoxP3+ TREG cells in the lungs of infected mice. Thus, this vaccination strategy may prove useful in limiting future pandemics by potentiating the long-term efficacy of mucosal vaccines within the context of the widely distributed BCG vaccine.

Original languageEnglish (US)
Article number107612
JournaliScience
Volume26
Issue number9
DOIs
StatePublished - Sep 15 2023
Externally publishedYes

Keywords

  • Biological sciences
  • Immune response
  • Immunology
  • Microbiology

ASJC Scopus subject areas

  • General

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