BCG administration promotes the long-term protection afforded by a single-dose intranasal adenovirus-based SARS-CoV-2 vaccine

Dilhan J. Perera; Pilar Domenech; George Giorgi Babuadze; Maedeh Naghibosadat; Fernando Alvarez; Cal Koger-Pease; Lydia Labrie; Matthew Stuible; Yves Durocher; Ciriaco A. Piccirillo; André Lametti; Pierre Olivier Fiset; Seyyed Mehdy Elahi; Gary P. Kobinger; Rénald Gilbert; Martin Olivier; Robert Kozak; Michael B. Reed; Momar Ndao

doi:10.1016/j.isci.2023.107612

BCG administration promotes the long-term protection afforded by a single-dose intranasal adenovirus-based SARS-CoV-2 vaccine

Dilhan J. Perera
, Pilar Domenech
, George Giorgi Babuadze
, Maedeh Naghibosadat
, Fernando Alvarez
, Cal Koger-Pease
, Lydia Labrie
, Matthew Stuible
, Yves Durocher
, Ciriaco A. Piccirillo
, André Lametti
, Pierre Olivier Fiset
, Seyyed Mehdy Elahi
, Gary P. Kobinger
, Rénald Gilbert
, Martin Olivier
, Robert Kozak
, Michael B. Reed
, Momar Ndao

Microbiology And Immunology

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Recent publications have explored intranasal (i.n.) adenovirus-based (Ad) vaccines as an effective strategy for SARS-CoV-2 in pre-clinical models. However, the effects of prior immunizations and infections have yet to be considered. Here, we investigate the immunomodulatory effects of Mycobacterium bovis BCG pre-immunization followed by vaccination with an S-protein-expressing i.n. Ad, termed Ad(Spike). While i.n. Ad(Spike) retains some protective effect after 6 months, a single administration of BCG-Danish prior to Ad(Spike) potentiates its ability to control viral replication of the B.1.351 SARS-CoV-2 variant within the respiratory tract. Though BCG-Danish did not affect Ad(Spike)-generated humoral immunity, it promoted the generation of cytotoxic/Th1 responses over suppressive FoxP3⁺ T_REG cells in the lungs of infected mice. Thus, this vaccination strategy may prove useful in limiting future pandemics by potentiating the long-term efficacy of mucosal vaccines within the context of the widely distributed BCG vaccine.

Original language	English (US)
Article number	107612
Journal	iScience
Volume	26
Issue number	9
DOIs	https://doi.org/10.1016/j.isci.2023.107612
State	Published - Sep 15 2023

Keywords

Biological sciences
Immune response
Immunology
Microbiology

ASJC Scopus subject areas

General

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10.1016/j.isci.2023.107612

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Perera, D. J., Domenech, P., Babuadze, G. G., Naghibosadat, M., Alvarez, F., Koger-Pease, C., Labrie, L., Stuible, M., Durocher, Y., Piccirillo, C. A., Lametti, A., Fiset, P. O., Elahi, S. M., Kobinger, G. P., Gilbert, R., Olivier, M., Kozak, R., Reed, M. B., & Ndao, M. (2023). BCG administration promotes the long-term protection afforded by a single-dose intranasal adenovirus-based SARS-CoV-2 vaccine. iScience, 26(9), Article 107612. https://doi.org/10.1016/j.isci.2023.107612

Perera, DJ, Domenech, P, Babuadze, GG, Naghibosadat, M, Alvarez, F, Koger-Pease, C, Labrie, L, Stuible, M, Durocher, Y, Piccirillo, CA, Lametti, A, Fiset, PO, Elahi, SM, Kobinger, GP, Gilbert, R, Olivier, M, Kozak, R, Reed, MB & Ndao, M 2023, 'BCG administration promotes the long-term protection afforded by a single-dose intranasal adenovirus-based SARS-CoV-2 vaccine', iScience, vol. 26, no. 9, 107612. https://doi.org/10.1016/j.isci.2023.107612

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abstract = "Recent publications have explored intranasal (i.n.) adenovirus-based (Ad) vaccines as an effective strategy for SARS-CoV-2 in pre-clinical models. However, the effects of prior immunizations and infections have yet to be considered. Here, we investigate the immunomodulatory effects of Mycobacterium bovis BCG pre-immunization followed by vaccination with an S-protein-expressing i.n. Ad, termed Ad(Spike). While i.n. Ad(Spike) retains some protective effect after 6 months, a single administration of BCG-Danish prior to Ad(Spike) potentiates its ability to control viral replication of the B.1.351 SARS-CoV-2 variant within the respiratory tract. Though BCG-Danish did not affect Ad(Spike)-generated humoral immunity, it promoted the generation of cytotoxic/Th1 responses over suppressive FoxP3+ TREG cells in the lungs of infected mice. Thus, this vaccination strategy may prove useful in limiting future pandemics by potentiating the long-term efficacy of mucosal vaccines within the context of the widely distributed BCG vaccine.",

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author = "Perera, \{Dilhan J.\} and Pilar Domenech and Babuadze, \{George Giorgi\} and Maedeh Naghibosadat and Fernando Alvarez and Cal Koger-Pease and Lydia Labrie and Matthew Stuible and Yves Durocher and Piccirillo, \{Ciriaco A.\} and Andr{\'e} Lametti and Fiset, \{Pierre Olivier\} and Elahi, \{Seyyed Mehdy\} and Kobinger, \{Gary P.\} and R{\'e}nald Gilbert and Martin Olivier and Robert Kozak and Reed, \{Michael B.\} and Momar Ndao",

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AU - Babuadze, George Giorgi

AU - Naghibosadat, Maedeh

AU - Alvarez, Fernando

AU - Koger-Pease, Cal

AU - Labrie, Lydia

AU - Stuible, Matthew

AU - Durocher, Yves

AU - Piccirillo, Ciriaco A.

AU - Lametti, André

AU - Fiset, Pierre Olivier

AU - Elahi, Seyyed Mehdy

AU - Kobinger, Gary P.

AU - Gilbert, Rénald

AU - Olivier, Martin

AU - Kozak, Robert

AU - Reed, Michael B.

AU - Ndao, Momar

PY - 2023/9/15

Y1 - 2023/9/15

N2 - Recent publications have explored intranasal (i.n.) adenovirus-based (Ad) vaccines as an effective strategy for SARS-CoV-2 in pre-clinical models. However, the effects of prior immunizations and infections have yet to be considered. Here, we investigate the immunomodulatory effects of Mycobacterium bovis BCG pre-immunization followed by vaccination with an S-protein-expressing i.n. Ad, termed Ad(Spike). While i.n. Ad(Spike) retains some protective effect after 6 months, a single administration of BCG-Danish prior to Ad(Spike) potentiates its ability to control viral replication of the B.1.351 SARS-CoV-2 variant within the respiratory tract. Though BCG-Danish did not affect Ad(Spike)-generated humoral immunity, it promoted the generation of cytotoxic/Th1 responses over suppressive FoxP3+ TREG cells in the lungs of infected mice. Thus, this vaccination strategy may prove useful in limiting future pandemics by potentiating the long-term efficacy of mucosal vaccines within the context of the widely distributed BCG vaccine.

AB - Recent publications have explored intranasal (i.n.) adenovirus-based (Ad) vaccines as an effective strategy for SARS-CoV-2 in pre-clinical models. However, the effects of prior immunizations and infections have yet to be considered. Here, we investigate the immunomodulatory effects of Mycobacterium bovis BCG pre-immunization followed by vaccination with an S-protein-expressing i.n. Ad, termed Ad(Spike). While i.n. Ad(Spike) retains some protective effect after 6 months, a single administration of BCG-Danish prior to Ad(Spike) potentiates its ability to control viral replication of the B.1.351 SARS-CoV-2 variant within the respiratory tract. Though BCG-Danish did not affect Ad(Spike)-generated humoral immunity, it promoted the generation of cytotoxic/Th1 responses over suppressive FoxP3+ TREG cells in the lungs of infected mice. Thus, this vaccination strategy may prove useful in limiting future pandemics by potentiating the long-term efficacy of mucosal vaccines within the context of the widely distributed BCG vaccine.

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KW - Immune response

KW - Immunology

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BCG administration promotes the long-term protection afforded by a single-dose intranasal adenovirus-based SARS-CoV-2 vaccine

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