Bcl-xL expression after contusion to the rat spinal cord

J. Qiu, O. Nesic, Z. Ye, H. Rea, K. N. Westlund, G. Y. Xu, D. McAdoo, C. E. Hulsebosch, J. R. Perez-Polo

Research output: Contribution to journalArticle

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Abstract

After contusion-derived spinal cord injury, (SCI) there is localized tissue disruption and energy failure that results in early necrosis and delayed apoptosis, events that contribute to chronic central pain in a majority of patients. We assessed the extent of contusion-induced apoptosis of neurons in a known central pain-signaling pathway, the spinothalamic tract (STT), which may be a contributor to SCI-induced pain. We observed the loss of STT cells and localized increase of DNA fragmentation and cytoplasmic histone-DNA complexes, which suggested potential apoptotic changes among STT neurons after SCI. We also showed SCI-associated changes in the expression of the antiapoptotic protein Bcl-xL, especially among STT cells, consistent with the hypothesis that Bcl-xL regulates the extent of apoptosis after SCI. Apoptosis in the injured spinal cord correlated well with prompt decreases in Bcl-xL protein levels and Bcl-xL/Bax protein ratios at the contusion site. We interpret these results as evidence that regulation of Bcl-xL may play a role in neural sparing after spinal injury and pain-signaling function.

Original languageEnglish (US)
Pages (from-to)1267-1278
Number of pages12
JournalJournal of Neurotrauma
Volume18
Issue number11
StatePublished - 2001
Externally publishedYes

Fingerprint

Spinothalamic Tracts
Contusions
Spinal Cord Injuries
Spinal Cord
Apoptosis
Pain
Neurons
bcl-2-Associated X Protein
Spinal Injuries
DNA Fragmentation
Chronic Pain
Histones
Proteins
Necrosis
DNA

Keywords

  • Apoptosis
  • Bcl-x
  • Chronic central pain (CCP)
  • Spinal cord injury (SCI)
  • Spinothalamic tract (STT)

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Qiu, J., Nesic, O., Ye, Z., Rea, H., Westlund, K. N., Xu, G. Y., ... Perez-Polo, J. R. (2001). Bcl-xL expression after contusion to the rat spinal cord. Journal of Neurotrauma, 18(11), 1267-1278.

Bcl-xL expression after contusion to the rat spinal cord. / Qiu, J.; Nesic, O.; Ye, Z.; Rea, H.; Westlund, K. N.; Xu, G. Y.; McAdoo, D.; Hulsebosch, C. E.; Perez-Polo, J. R.

In: Journal of Neurotrauma, Vol. 18, No. 11, 2001, p. 1267-1278.

Research output: Contribution to journalArticle

Qiu, J, Nesic, O, Ye, Z, Rea, H, Westlund, KN, Xu, GY, McAdoo, D, Hulsebosch, CE & Perez-Polo, JR 2001, 'Bcl-xL expression after contusion to the rat spinal cord', Journal of Neurotrauma, vol. 18, no. 11, pp. 1267-1278.
Qiu J, Nesic O, Ye Z, Rea H, Westlund KN, Xu GY et al. Bcl-xL expression after contusion to the rat spinal cord. Journal of Neurotrauma. 2001;18(11):1267-1278.
Qiu, J. ; Nesic, O. ; Ye, Z. ; Rea, H. ; Westlund, K. N. ; Xu, G. Y. ; McAdoo, D. ; Hulsebosch, C. E. ; Perez-Polo, J. R. / Bcl-xL expression after contusion to the rat spinal cord. In: Journal of Neurotrauma. 2001 ; Vol. 18, No. 11. pp. 1267-1278.
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