Bead-based approaches to develop thioaptamers for diagnostics and therapeutics

Xianbin Yang; Duane Beasley; Johnnie Engelhardt; Mark Shumbera; Bruce A. Luxon; David G. Gorenstein

doi:10.1080/10426500701761367

Bead-based approaches to develop thioaptamers for diagnostics and therapeutics

Xianbin Yang, Duane Beasley, Johnnie Engelhardt, Mark Shumbera, Bruce A. Luxon, David G. Gorenstein

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

In this presentation, we will outline our novel, integrated bead-based oligonucleotide selection procedures including the automation of the split-pool synthesis of a one-bead one-thioaptamer library, high-throughput flow cytometric sorting and a PCR-based sequencing. Additionally, we will also discuss one promising thioaptamer that has been shown in preliminary animal therapeutic dosing to increase survival in animal models of infection with West Nile virus. Moreover, we will present our ongoing work on a thioaptamer proteomics chip.

Original language	English (US)
Pages (from-to)	469-472
Number of pages	4
Journal	Phosphorus, Sulfur and Silicon and the Related Elements
Volume	183
Issue number	2-3
DOIs	https://doi.org/10.1080/10426500701761367
State	Published - Feb 2008
Externally published	Yes

Keywords

Aptamer
Bead
Combinatorial chemistry
Oligonucleoside phosphorodithioate
Oligonucleoside phosphorothioate
Thioaptamer

ASJC Scopus subject areas

Biochemistry
Organic Chemistry
Inorganic Chemistry

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10.1080/10426500701761367

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title = "Bead-based approaches to develop thioaptamers for diagnostics and therapeutics",

abstract = "In this presentation, we will outline our novel, integrated bead-based oligonucleotide selection procedures including the automation of the split-pool synthesis of a one-bead one-thioaptamer library, high-throughput flow cytometric sorting and a PCR-based sequencing. Additionally, we will also discuss one promising thioaptamer that has been shown in preliminary animal therapeutic dosing to increase survival in animal models of infection with West Nile virus. Moreover, we will present our ongoing work on a thioaptamer proteomics chip.",

keywords = "Aptamer, Bead, Combinatorial chemistry, Oligonucleoside phosphorodithioate, Oligonucleoside phosphorothioate, Thioaptamer",

author = "Xianbin Yang and Duane Beasley and Johnnie Engelhardt and Mark Shumbera and Luxon, {Bruce A.} and Gorenstein, {David G.}",

note = "Funding Information: This research was supported by grants to Dr. Xianbin Yang from Mike Hogg Fund and NIH (1R03CA121353-01); to Dr. David Gorenstein from DARPA (P42296LS0000), NIH (U01 A1054827, IP30 ES06676; N01 HV28184; UO1 AI 60616), DoD (DADD13-02-C-007 and contract number W911 SR-04-C-0065 with the U.S. Edgewood Chemical Biological Center), the Welch Foundation (H-1296), and the State of Texas Advanced Technology Program (004952-0038-2003).",

year = "2008",

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doi = "10.1080/10426500701761367",

language = "English (US)",

volume = "183",

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AU - Yang, Xianbin

AU - Beasley, Duane

AU - Engelhardt, Johnnie

AU - Shumbera, Mark

AU - Luxon, Bruce A.

AU - Gorenstein, David G.

N1 - Funding Information: This research was supported by grants to Dr. Xianbin Yang from Mike Hogg Fund and NIH (1R03CA121353-01); to Dr. David Gorenstein from DARPA (P42296LS0000), NIH (U01 A1054827, IP30 ES06676; N01 HV28184; UO1 AI 60616), DoD (DADD13-02-C-007 and contract number W911 SR-04-C-0065 with the U.S. Edgewood Chemical Biological Center), the Welch Foundation (H-1296), and the State of Texas Advanced Technology Program (004952-0038-2003).

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AB - In this presentation, we will outline our novel, integrated bead-based oligonucleotide selection procedures including the automation of the split-pool synthesis of a one-bead one-thioaptamer library, high-throughput flow cytometric sorting and a PCR-based sequencing. Additionally, we will also discuss one promising thioaptamer that has been shown in preliminary animal therapeutic dosing to increase survival in animal models of infection with West Nile virus. Moreover, we will present our ongoing work on a thioaptamer proteomics chip.

KW - Aptamer

KW - Bead

KW - Combinatorial chemistry

KW - Oligonucleoside phosphorodithioate

KW - Oligonucleoside phosphorothioate

KW - Thioaptamer

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Bead-based approaches to develop thioaptamers for diagnostics and therapeutics

Abstract

Keywords

ASJC Scopus subject areas

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