Bead-based approaches to develop thioaptamers for diagnostics and therapeutics

Xianbin Yang, Duane Beasley, Johnnie Engelhardt, Mark Shumbera, Bruce A. Luxon, David G. Gorenstein

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    In this presentation, we will outline our novel, integrated bead-based oligonucleotide selection procedures including the automation of the split-pool synthesis of a one-bead one-thioaptamer library, high-throughput flow cytometric sorting and a PCR-based sequencing. Additionally, we will also discuss one promising thioaptamer that has been shown in preliminary animal therapeutic dosing to increase survival in animal models of infection with West Nile virus. Moreover, we will present our ongoing work on a thioaptamer proteomics chip.

    Original languageEnglish (US)
    Pages (from-to)469-472
    Number of pages4
    JournalPhosphorus, Sulfur and Silicon and the Related Elements
    Volume183
    Issue number2-3
    DOIs
    StatePublished - Feb 2008

    Fingerprint

    West Nile virus
    Automation
    Oligonucleotides
    Proteomics
    Libraries
    Animals
    Animal Models
    Polymerase Chain Reaction
    Infection
    Viruses
    Sorting
    Throughput
    Therapeutics

    Keywords

    • Aptamer
    • Bead
    • Combinatorial chemistry
    • Oligonucleoside phosphorodithioate
    • Oligonucleoside phosphorothioate
    • Thioaptamer

    ASJC Scopus subject areas

    • Inorganic Chemistry

    Cite this

    Yang, X., Beasley, D., Engelhardt, J., Shumbera, M., Luxon, B. A., & Gorenstein, D. G. (2008). Bead-based approaches to develop thioaptamers for diagnostics and therapeutics. Phosphorus, Sulfur and Silicon and the Related Elements, 183(2-3), 469-472. https://doi.org/10.1080/10426500701761367

    Bead-based approaches to develop thioaptamers for diagnostics and therapeutics. / Yang, Xianbin; Beasley, Duane; Engelhardt, Johnnie; Shumbera, Mark; Luxon, Bruce A.; Gorenstein, David G.

    In: Phosphorus, Sulfur and Silicon and the Related Elements, Vol. 183, No. 2-3, 02.2008, p. 469-472.

    Research output: Contribution to journalArticle

    Yang, X, Beasley, D, Engelhardt, J, Shumbera, M, Luxon, BA & Gorenstein, DG 2008, 'Bead-based approaches to develop thioaptamers for diagnostics and therapeutics', Phosphorus, Sulfur and Silicon and the Related Elements, vol. 183, no. 2-3, pp. 469-472. https://doi.org/10.1080/10426500701761367
    Yang, Xianbin ; Beasley, Duane ; Engelhardt, Johnnie ; Shumbera, Mark ; Luxon, Bruce A. ; Gorenstein, David G. / Bead-based approaches to develop thioaptamers for diagnostics and therapeutics. In: Phosphorus, Sulfur and Silicon and the Related Elements. 2008 ; Vol. 183, No. 2-3. pp. 469-472.
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