Bead-based approaches to develop thioaptamers for diagnostics and therapeutics

Xianbin Yang, Duane Beasley, Johnnie Engelhardt, Mark Shumbera, Bruce A. Luxon, David G. Gorenstein

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

In this presentation, we will outline our novel, integrated bead-based oligonucleotide selection procedures including the automation of the split-pool synthesis of a one-bead one-thioaptamer library, high-throughput flow cytometric sorting and a PCR-based sequencing. Additionally, we will also discuss one promising thioaptamer that has been shown in preliminary animal therapeutic dosing to increase survival in animal models of infection with West Nile virus. Moreover, we will present our ongoing work on a thioaptamer proteomics chip.

Original languageEnglish (US)
Pages (from-to)469-472
Number of pages4
JournalPhosphorus, Sulfur and Silicon and the Related Elements
Volume183
Issue number2-3
DOIs
StatePublished - Feb 1 2008

Keywords

  • Aptamer
  • Bead
  • Combinatorial chemistry
  • Oligonucleoside phosphorodithioate
  • Oligonucleoside phosphorothioate
  • Thioaptamer

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry
  • Inorganic Chemistry

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    Yang, X., Beasley, D., Engelhardt, J., Shumbera, M., Luxon, B. A., & Gorenstein, D. G. (2008). Bead-based approaches to develop thioaptamers for diagnostics and therapeutics. Phosphorus, Sulfur and Silicon and the Related Elements, 183(2-3), 469-472. https://doi.org/10.1080/10426500701761367