Belgrade virus: A new hantavirus causing severe hemorrhagic fever with renal syndrome in yugoslavia

Ana Gligic, Nada Dimkovic, Shu Yuan Xiao, Guy J. Buckle, Dragan Jovanovic, Dragisa Velimirovic, Radivoj Stojanovic, Mirceta Obradovic, Gordana Diglisic, Jasmina Micic, David M. Asher, James W. LcDuc, Richard Yanagihara, D. Carleton Gajdusek

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Abstract

Two biologically and genetically distinct hantaviruses were isolated from blood and urine specimens collected from four Yugoslavian patients with clinically severe hemorrhagic fever with renal syndrome (HFRS). Viral isolates from three patients, designated strains Belgrade 1-3, were distinct from Hantaan, Seoul, Puumala, and Prospect Hill viruses as determined by plaque- reduction neutralization tests and restriction analysis of enzymatically amplified M-segment fragments. The fourth isolate, called Kraljevo, was indistinguishable from Hantaan virus. Strains Belgrade 1 and 2, like the Kraljevo strain, caused a fatal meningoencephalitis in newborn mice inoculated with 100 pfu of virus intracerebrally and intraperitoneally. Strain Belgrade 3 was much less neurovirulent, requiring 30,000 pfu of virus to cause fatal disease in mice. These data indicate that two distinct hantaviruses, one of which constitutes a new serotype, cause clinically severe HFRS in Yugoslavia.

Original languageEnglish (US)
Pages (from-to)113-120
Number of pages8
JournalJournal of Infectious Diseases
Volume166
Issue number1
DOIs
StatePublished - Jul 1992
Externally publishedYes

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

Cite this

Gligic, A., Dimkovic, N., Xiao, S. Y., Buckle, G. J., Jovanovic, D., Velimirovic, D., Stojanovic, R., Obradovic, M., Diglisic, G., Micic, J., Asher, D. M., LcDuc, J. W., Yanagihara, R., & Carleton Gajdusek, D. (1992). Belgrade virus: A new hantavirus causing severe hemorrhagic fever with renal syndrome in yugoslavia. Journal of Infectious Diseases, 166(1), 113-120. https://doi.org/10.1093/infdis/166.1.113