Benzalkonium chloride causes colposcopic changes and increased susceptibility to genital herpes infection in mice

Kathleen L. Vincent, Brent A. Bell, Rebecca K. Johnston, Rachael Stegall, Gracie Vargas, Alai Tan, Lawrence R. Stanberry, Susan L. Rosenthal, Gregg N. Milligan, Massoud Motamedi, Nigel Bourne

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Background: Colposcopy is widely used in clinical microbicide safety testing but not in preclinical small animal studies. Endoscopic colposcopy could be employed in small animals allowing colposcopy to be used as one component in a multifactorial safety testing paradigm. Study design: We conducted dose-response studies in mice using 2%, 0.2%, or 0.02% benzalkonium chloride (BZK) as the test compound, and using multiple safety end points that included endoscopic colposcopy, susceptibility to vaginal HSV-2 infection, histology, and entry of inflammatory cells into the vagina. Results: Animals treated with 0.2% or higher BZK experienced vaginal toxicities detectable by all tests used including colposcopy. In contrast, 0.02% BZK produced no significant changes except by histology in which a significant thinning of the vaginal epithelium was seen. Conclusion: Endoscopic colposcopy detected microbicide-elicited changes in the mouse vagina with similar sensitivity to the other endpoints used in these studies and would appear to be useful as part of a multifactorial microbicide safety testing paradigm in mice.

Original languageEnglish (US)
Pages (from-to)579-584
Number of pages6
JournalSexually Transmitted Diseases
Volume37
Issue number9
DOIs
StatePublished - Sep 1 2010

    Fingerprint

ASJC Scopus subject areas

  • Dermatology
  • Public Health, Environmental and Occupational Health
  • Microbiology (medical)
  • Infectious Diseases

Cite this