Beta-adrenergic receptor differences in cultured arterial smooth muscle cells between spontaneously hypertensive and Wistar-Kyoto rats

A. Jazayeri, Walter Meyer

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In this study, β-adrenergic receptors of cultured arterial smooth muscle cells (ASMC) from normotensive Wistar-Kyoto rats (WKY) and spontaneously hypertensive rats (SHR) were compared. Scatchard analysis of [3H]-dihydroalprenolol hydrochloride (DHA) binding revealed that the maximum binding capacity (B(max) for [3H]-DHA binding was significantly higher in WKY than in SHR, by twofold (n = 6, P < 0.0001). Analysis of isoproterenol competition for [3H]-DHA binding by computerized non-linear regression indicated that the two strains have an equal number of high-affinity-state receptors, but that WKY rats have about three times more low-affinity-state receptors than SHR. Isoproterenol stimulated cyclic adenosine monophosphate (cAMP) production in a dose-dependent manner, and dose-response experiments revealed an effective concentration of ligand at which 50% of the maximum effect is observed (EC50) of 5-10 x 10-8 mol/l isoproterenol for both WKY and SHR. Also, isoproterenol-stimulated cAMP production was inhibited by propranolol in a dose-dependent manner. This study demonstrates that the previously described difference observed for in vivo binding of β-adrenergic receptors of vessels from SHR is retained in tissue culture of vascular smooth muscle and is, therefore, independent of the elevated plasma catecholamine and blood pressure levels found in SHR. The finding that there is equal cAMP stimulation in WKY and SHR is consistent with the discovery of an equal number of high-affinity-state receptors in the two strains. The difference in total β-adrenergic receptor number is secondary to the greater number of low-affinity-state receptors in WKY.

Original languageEnglish (US)
Pages (from-to)895-900
Number of pages6
JournalJournal of Hypertension
Volume7
Issue number11
DOIs
StatePublished - 1989

Fingerprint

Inbred WKY Rats
Receptors, Adrenergic, beta
Inbred SHR Rats
Smooth Muscle Myocytes
Dihydroalprenolol
Isoproterenol
Cyclic AMP
Adrenergic Receptors
Vascular Smooth Muscle
Propranolol
Catecholamines
Blood Pressure
Ligands

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology

Cite this

Beta-adrenergic receptor differences in cultured arterial smooth muscle cells between spontaneously hypertensive and Wistar-Kyoto rats. / Jazayeri, A.; Meyer, Walter.

In: Journal of Hypertension, Vol. 7, No. 11, 1989, p. 895-900.

Research output: Contribution to journalArticle

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