Binding constants of soluble NGF-receptors in rat oligodendrocytes and astrocytes in culture

Dario Marchetti; Robert W. Stach; Russell Saneto; Jean de Vellis; J. Regino Perez-Polo

doi:10.1016/S0006-291X(87)80138-9

Binding constants of soluble NGF-receptors in rat oligodendrocytes and astrocytes in culture

Dario Marchetti, Robert W. Stach, Russell Saneto, Jean de Vellis, J. Regino Perez-Polo

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

The neuronotrophic factor NGF binds to peripheral neurons of the dorsal root ganglion and the sympathetic nervous system. NGF binds to a cell surface receptor, NGFR, on these cells and displays Kd's of 10^-9 and 10^-11M. NGF receptors have also been reported for basal forebrain magnocellular neurons. In addition, NGF specifically binds to NGFR on Schwann cells although the biological significance of this binding is not known. Here we report that NGF binds in a saturable and specific fashion to receptors on cultured isolated populations of rat astrocytes but not to oligodendrocytes. The binding to astrocytes in culture displayed a Kd of 2.7 ± 1.0 nM with 36,000 receptors per cell.

Original language	English (US)
Pages (from-to)	422-427
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	147
Issue number	1
DOIs	https://doi.org/10.1016/S0006-291X(87)80138-9
State	Published - Aug 31 1987
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/S0006-291X(87)80138-9

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title = "Binding constants of soluble NGF-receptors in rat oligodendrocytes and astrocytes in culture",

abstract = "The neuronotrophic factor NGF binds to peripheral neurons of the dorsal root ganglion and the sympathetic nervous system. NGF binds to a cell surface receptor, NGFR, on these cells and displays Kd's of 10-9 and 10-11M. NGF receptors have also been reported for basal forebrain magnocellular neurons. In addition, NGF specifically binds to NGFR on Schwann cells although the biological significance of this binding is not known. Here we report that NGF binds in a saturable and specific fashion to receptors on cultured isolated populations of rat astrocytes but not to oligodendrocytes. The binding to astrocytes in culture displayed a Kd of 2.7 ± 1.0 nM with 36,000 receptors per cell.",

author = "Dario Marchetti and Stach, {Robert W.} and Russell Saneto and {de Vellis}, Jean and Perez-Polo, {J. Regino}",

note = "Funding Information: ACKNOWLEDGEMENTS. This work was supported in part by NINCDS grant NS18708 (JRP-P) and USPH HDHD-06576 (JV). Thanks to D. Masters for manuscript preparation.",

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T1 - Binding constants of soluble NGF-receptors in rat oligodendrocytes and astrocytes in culture

AU - Marchetti, Dario

AU - Stach, Robert W.

AU - Saneto, Russell

AU - de Vellis, Jean

AU - Perez-Polo, J. Regino

N1 - Funding Information: ACKNOWLEDGEMENTS. This work was supported in part by NINCDS grant NS18708 (JRP-P) and USPH HDHD-06576 (JV). Thanks to D. Masters for manuscript preparation.

PY - 1987/8/31

Y1 - 1987/8/31

N2 - The neuronotrophic factor NGF binds to peripheral neurons of the dorsal root ganglion and the sympathetic nervous system. NGF binds to a cell surface receptor, NGFR, on these cells and displays Kd's of 10-9 and 10-11M. NGF receptors have also been reported for basal forebrain magnocellular neurons. In addition, NGF specifically binds to NGFR on Schwann cells although the biological significance of this binding is not known. Here we report that NGF binds in a saturable and specific fashion to receptors on cultured isolated populations of rat astrocytes but not to oligodendrocytes. The binding to astrocytes in culture displayed a Kd of 2.7 ± 1.0 nM with 36,000 receptors per cell.

AB - The neuronotrophic factor NGF binds to peripheral neurons of the dorsal root ganglion and the sympathetic nervous system. NGF binds to a cell surface receptor, NGFR, on these cells and displays Kd's of 10-9 and 10-11M. NGF receptors have also been reported for basal forebrain magnocellular neurons. In addition, NGF specifically binds to NGFR on Schwann cells although the biological significance of this binding is not known. Here we report that NGF binds in a saturable and specific fashion to receptors on cultured isolated populations of rat astrocytes but not to oligodendrocytes. The binding to astrocytes in culture displayed a Kd of 2.7 ± 1.0 nM with 36,000 receptors per cell.

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Binding constants of soluble NGF-receptors in rat oligodendrocytes and astrocytes in culture

Abstract

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