Binding of basic fibroblast growth factor to fibrinogen and fibrin*

Abha Sahni, Tatjana Odrljin, Charles W. Francis

Research output: Contribution to journalArticle

185 Citations (Scopus)

Abstract

Fibrin is formed at sites of tissue injury and provides the temporary matrix needed to support the initial endothelial cell responses needed for vessel repair. Basic fibroblast growth factor (bFGF) also acts at sites of injury and stimulates similar vascular cell responses. We have, therefore, investigated whether there are specific interactions between bFGF and fibrinogen and fibrin that could play a role in coordinating these actions. Binding studies were performed using bFGF immobilized on Sepharose beads and soluble 125I-labeled fibrinogen and also using Sepharose-immobilized fibrinogen and soluble 125I-bFGF. Both systems demonstrated specific and saturable binding. Scatchard analysis indicated two classes of binding sites for each with K(d) values of 1.3 and 260 nM using immobilized bFGF; and K(d) values of 0.9 and 70 nM using immobilized fibrinogen. After conversion of Sepharose-immobilized fibrinogen to fibrin by treatment with thrombin, bFGF also demonstrated specific and saturable binding with two classes of binding sites having K(d) values of 0.13 and 83 nM. Fibrin binding was also investigated by clotting a solution of bFGF and fibrinogen, and two classes of binding sites were demonstrated using this system with K(d) values of 0.8 and 261 nM. The maximum molar binding ratios of bFGF to fibrinogen were between 2.0 and 4.0 with the four binding systems. We conclude that bFGF binds specifically and saturably to fibrinogen and fibrin with high affinity, and this may have implications regarding the localization of its effect at sites of tissue injury.

Original languageEnglish (US)
Pages (from-to)7554-7559
Number of pages6
JournalJournal of Biological Chemistry
Volume273
Issue number13
DOIs
StatePublished - Mar 27 1998
Externally publishedYes

Fingerprint

Fibroblast Growth Factor 2
Fibrin
Fibrinogen
Sepharose
Binding Sites
Wounds and Injuries
Tissue
Endothelial cells
Thrombin
Blood Vessels
Repair
Endothelial Cells

ASJC Scopus subject areas

  • Biochemistry

Cite this

Binding of basic fibroblast growth factor to fibrinogen and fibrin*. / Sahni, Abha; Odrljin, Tatjana; Francis, Charles W.

In: Journal of Biological Chemistry, Vol. 273, No. 13, 27.03.1998, p. 7554-7559.

Research output: Contribution to journalArticle

Sahni, Abha ; Odrljin, Tatjana ; Francis, Charles W. / Binding of basic fibroblast growth factor to fibrinogen and fibrin*. In: Journal of Biological Chemistry. 1998 ; Vol. 273, No. 13. pp. 7554-7559.
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