Binding of cationic cell-permeable peptides to plastic and glass

Diane E. Chico, Randall L. Given, Brian T. Miller

    Research output: Contribution to journalArticle

    31 Citations (Scopus)

    Abstract

    Cell-penetrating peptides derived from hydrophilic regions of the homeoprotein Antennapedia (Antp) or the transcription-regulating factor Tat have been used to transport several peptide and oligonucleotide cargoes into the interior of cells. Such vector peptides penetrate cells, in part, because they contain multiple lysine and arginine residues. Using radiolabeled peptide cargoes covalently linked to Antp- or Tat-related vectors, or to D-Arg heptamers, we found that a significant amount of the label remained tightly bound to plastic and glass surfaces. Binding of the labeled conjugates was due entirely to the cationic vector moieties. Under certain conditions, such non-specific binding could be mistaken for cellular penetration.

    Original languageEnglish (US)
    Pages (from-to)3-9
    Number of pages7
    JournalPeptides
    Volume24
    Issue number1
    DOIs
    StatePublished - Jan 1 2003

    Fingerprint

    Plastics
    Glass
    Peptides
    Cell-Penetrating Peptides
    Homeodomain Proteins
    Transcription
    Oligonucleotides
    Lysine
    Arginine
    Labels
    Transcription Factors

    Keywords

    • Antennapedia
    • Glass adsorption
    • Plastic adsorption
    • Poly-D-arginine
    • Radioiodination
    • Tat
    • Vector peptides

    ASJC Scopus subject areas

    • Biochemistry
    • Endocrinology
    • Physiology
    • Cellular and Molecular Neuroscience

    Cite this

    Binding of cationic cell-permeable peptides to plastic and glass. / Chico, Diane E.; Given, Randall L.; Miller, Brian T.

    In: Peptides, Vol. 24, No. 1, 01.01.2003, p. 3-9.

    Research output: Contribution to journalArticle

    Chico, Diane E. ; Given, Randall L. ; Miller, Brian T. / Binding of cationic cell-permeable peptides to plastic and glass. In: Peptides. 2003 ; Vol. 24, No. 1. pp. 3-9.
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