Binding of insulin-like growth factor-I to rat uterus; variations during sensitization and decidualization

Y. Chandrasekhar, S. Narayan, Pomila Singh, M. Nagamani

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

IGF-I receptors have been identified and characterized in a variety of tissues. In this study receptors for IGF-I in the rat uterine tissue were identified and characterized. We have demonstrated IGF-I receptors in crude uterine membranes by binding and cross-linking experiments. IGF-I binding to the rat uterine membranes displayed time, temperature and pH dependence, and optimal binding conditions were achieved by 20 h of incubation at 4°C, at a pH of 7.8. Uterine IGF-I binding sites were specific for binding IGF-I peptide and demonstrated less than 100 x lower affinity for insulin. The binding was reversible and Scatchard analysis indicated presence of a single class of binding sites with an apparent dissociation constant of 1.68 ± 0.24 nmol/l and B(max) of 0.82 ± 0.1 pmol/mg protein. During estrogen treatment, sensitization and decidualization there was an overall increase of membrane proteins in the uterus and a relative decrease of IGF-I receptors per unit of protein. When expressed on a per uterus basis, there was a progressive increment in total IGF-I binding in estradiol-treated, sensitized, and decidualized uterus compared with controls. These data indicate a possible role for IGF-I in uterine cell multiplication and further differentiation to decidual cells in response to deciduogenic stimuli.

Original languageEnglish (US)
Pages (from-to)243-250
Number of pages8
JournalActa Endocrinologica
Volume123
Issue number2
StatePublished - 1990

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Insulin-Like Growth Factor I
IGF Type 1 Receptor
Uterus
Binding Sites
Membranes
Estradiol
Estrogens
Membrane Proteins
Proteins
Cell Proliferation
Insulin
Peptides
Temperature

ASJC Scopus subject areas

  • Endocrinology

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Binding of insulin-like growth factor-I to rat uterus; variations during sensitization and decidualization. / Chandrasekhar, Y.; Narayan, S.; Singh, Pomila; Nagamani, M.

In: Acta Endocrinologica, Vol. 123, No. 2, 1990, p. 243-250.

Research output: Contribution to journalArticle

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