Biochemical and pharmacological studies on the opiate receptors from human placenta

Mahmoud Ahmed, Essam E. Abdelhamid, D. Turner

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The effect of different enzymatic treatments on the specific binding of 3H-Etorphine to the membrane bound opiate receptors from human placenta was studied. Phospholipiase A2 inhibited etorphine binding, suggesting either that phospholipids are essential for ligand binding or that products of the phospholipase A2 reaction, either lysophospholipids or fatty acids, disrupt the membrane integrity because of their detergent like properties. Trypsin and α-chymotrypsin had no effect, suggesting that the receptors binding site was not accessible to these proteolytic enzymes. We also find that the placental villus upon in vitro stimulation with potassium releases acetylcholine. The inhibitory effect of morphine on acetylcholine release was investigated.

Original languageEnglish (US)
Pages (from-to)207-210
Number of pages4
JournalLife Sciences
Volume33
Issue numberSUPPL. 1
DOIs
StatePublished - 1983
Externally publishedYes

Fingerprint

Etorphine
Opioid Receptors
Placenta
Acetylcholine
Pharmacology
Lysophospholipids
Membranes
Chorionic Villi
Phospholipases A2
varespladib methyl
Detergents
Morphine
Potassium
Peptide Hydrolases
Fatty Acids
Binding Sites
Ligands

ASJC Scopus subject areas

  • Pharmacology

Cite this

Biochemical and pharmacological studies on the opiate receptors from human placenta. / Ahmed, Mahmoud; Abdelhamid, Essam E.; Turner, D.

In: Life Sciences, Vol. 33, No. SUPPL. 1, 1983, p. 207-210.

Research output: Contribution to journalArticle

Ahmed, Mahmoud ; Abdelhamid, Essam E. ; Turner, D. / Biochemical and pharmacological studies on the opiate receptors from human placenta. In: Life Sciences. 1983 ; Vol. 33, No. SUPPL. 1. pp. 207-210.
@article{7a6df505c36247c396d828a2270b7a41,
title = "Biochemical and pharmacological studies on the opiate receptors from human placenta",
abstract = "The effect of different enzymatic treatments on the specific binding of 3H-Etorphine to the membrane bound opiate receptors from human placenta was studied. Phospholipiase A2 inhibited etorphine binding, suggesting either that phospholipids are essential for ligand binding or that products of the phospholipase A2 reaction, either lysophospholipids or fatty acids, disrupt the membrane integrity because of their detergent like properties. Trypsin and α-chymotrypsin had no effect, suggesting that the receptors binding site was not accessible to these proteolytic enzymes. We also find that the placental villus upon in vitro stimulation with potassium releases acetylcholine. The inhibitory effect of morphine on acetylcholine release was investigated.",
author = "Mahmoud Ahmed and Abdelhamid, {Essam E.} and D. Turner",
year = "1983",
doi = "10.1016/0024-3205(83)90479-4",
language = "English (US)",
volume = "33",
pages = "207--210",
journal = "Life Sciences",
issn = "0024-3205",
publisher = "Elsevier Inc.",
number = "SUPPL. 1",

}

TY - JOUR

T1 - Biochemical and pharmacological studies on the opiate receptors from human placenta

AU - Ahmed, Mahmoud

AU - Abdelhamid, Essam E.

AU - Turner, D.

PY - 1983

Y1 - 1983

N2 - The effect of different enzymatic treatments on the specific binding of 3H-Etorphine to the membrane bound opiate receptors from human placenta was studied. Phospholipiase A2 inhibited etorphine binding, suggesting either that phospholipids are essential for ligand binding or that products of the phospholipase A2 reaction, either lysophospholipids or fatty acids, disrupt the membrane integrity because of their detergent like properties. Trypsin and α-chymotrypsin had no effect, suggesting that the receptors binding site was not accessible to these proteolytic enzymes. We also find that the placental villus upon in vitro stimulation with potassium releases acetylcholine. The inhibitory effect of morphine on acetylcholine release was investigated.

AB - The effect of different enzymatic treatments on the specific binding of 3H-Etorphine to the membrane bound opiate receptors from human placenta was studied. Phospholipiase A2 inhibited etorphine binding, suggesting either that phospholipids are essential for ligand binding or that products of the phospholipase A2 reaction, either lysophospholipids or fatty acids, disrupt the membrane integrity because of their detergent like properties. Trypsin and α-chymotrypsin had no effect, suggesting that the receptors binding site was not accessible to these proteolytic enzymes. We also find that the placental villus upon in vitro stimulation with potassium releases acetylcholine. The inhibitory effect of morphine on acetylcholine release was investigated.

UR - http://www.scopus.com/inward/record.url?scp=0021050986&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021050986&partnerID=8YFLogxK

U2 - 10.1016/0024-3205(83)90479-4

DO - 10.1016/0024-3205(83)90479-4

M3 - Article

C2 - 6319862

AN - SCOPUS:0021050986

VL - 33

SP - 207

EP - 210

JO - Life Sciences

JF - Life Sciences

SN - 0024-3205

IS - SUPPL. 1

ER -