An analysis of the soluble proteins from the adult and calf bovine lens epithelial cells by DEAE-cellulose fractionation has shown that these cells do not contain the γ-crystallins, but do contain the normal complement of α-andβ-crystallins. Significant quantities of γ-crystallins could be found, however, in the fiber cells of adult, calf and embryonic lenses. It was concluded from these experiments that the initiation of γ-crystallin synthesis is specifically associated with the differentiation of lens epithelial cells to lens fiber cells. Resolution of teh γ-crystallin of adult cortex, adult nucleus and embryonic lens was achieved by fractionation on DEAE-cellulose columns. Electrophoretic analysis of the purified γ-crystallin showed that those of the embryo and adult nucleus have similar mobilities whereas the mobility of adult cortex γ-crystallins differed from the others. It appears, therefore that the γ-crystallins formed in fiber cell differentiation in an embryonic lens are electrophoretically distinct from those formed during fiber differentiation in an adult lens.
|Original language||English (US)|
|Number of pages||10|
|Journal||BBA - General Subjects|
|State||Published - Aug 24 1965|
ASJC Scopus subject areas
- Molecular Biology