Bioconjugated gold nanoparticles as a contrast agent for optoacoustic detection of small tumors

Mohammad A. Eghtedari, John A. Copland, Vsevolod Popov, Nicholas A. Kotov, Massoud Motamedi, Alexander A. Oraevsky

Research output: Chapter in Book/Report/Conference proceedingConference contribution

23 Citations (Scopus)

Abstract

Optoacoustic tomography (OAT) is a medical imaging method for detection of cancerous tumors that uses laser pulses to produce transi ultrasonic waves with spatial profiles replicating distribution of absorbed optical energy. Unlike conventional ultrasonography that uses an external source of acoustic waves. OAT uses transient acoustic waves generated as result of thermal expansion of tissue preferentially heared with short laser pulses. Tissues with different optical properties have different optoacoustic profiles and this enables reconstruction of an acoustic image based on distribution of optical absorption. It is anticipated than the difference in optical absorption between very early tumors and normal tissues might be minimal, justifying application of a contrast agent. Gold Nanoparticles (NP) can be designed to strongly absorb desirable color of laser pulses and effectively produce acoustic waves. Therefore, gold NP can be potentially employed as an optoacoustic contrast agent. We studied sensitivity of optoacoustic imaging in phantoms resembling dimensions and properties of the breast with small objects loaded with gold NPs of various concentrations. Targeted selective loading of breast cancer cells in culture with 40-nm diameter NPs was experimentally demonstrated with electron microscopy and fluorescence labeling techniques. To achieve selective targeting, Herceptin, a monoclonal antibody raised against Her2 receptor was conjugated to NPs using streptavidin-biotin conjugation as a linker. Targeting experiments simultaneously demonstrated than Mab/NPs conjugates inhibit cell proliferation of Her/neu positive cells. These data present the first step in development of a new technology for highly selective cancer chemotherapy with capability to diagnose the presence of malignant tumors and monitor the effects of the treatment.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsA.A. Oraevsky
Pages76-85
Number of pages10
Volume4960
DOIs
StatePublished - 2003
EventPROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Biomedical Optoacoutics IV - San Jose, CA, United States
Duration: Jan 26 2003Jan 27 2003

Other

OtherPROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Biomedical Optoacoutics IV
CountryUnited States
CitySan Jose, CA
Period1/26/031/27/03

Fingerprint

Photoacoustic effect
Tumors
tumors
Gold
gold
Nanoparticles
nanoparticles
acoustics
Laser pulses
breast
Acoustic waves
Tissue
optical absorption
tomography
pulses
cancer
Light absorption
Tomography
lasers
biotin

Keywords

  • Cancer
  • Gold nanoparticles
  • Herceptin
  • Imaging
  • Optoacoustic
  • Targeting

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Eghtedari, M. A., Copland, J. A., Popov, V., Kotov, N. A., Motamedi, M., & Oraevsky, A. A. (2003). Bioconjugated gold nanoparticles as a contrast agent for optoacoustic detection of small tumors. In A. A. Oraevsky (Ed.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 4960, pp. 76-85) https://doi.org/10.1117/12.503617

Bioconjugated gold nanoparticles as a contrast agent for optoacoustic detection of small tumors. / Eghtedari, Mohammad A.; Copland, John A.; Popov, Vsevolod; Kotov, Nicholas A.; Motamedi, Massoud; Oraevsky, Alexander A.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / A.A. Oraevsky. Vol. 4960 2003. p. 76-85.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Eghtedari, MA, Copland, JA, Popov, V, Kotov, NA, Motamedi, M & Oraevsky, AA 2003, Bioconjugated gold nanoparticles as a contrast agent for optoacoustic detection of small tumors. in AA Oraevsky (ed.), Proceedings of SPIE - The International Society for Optical Engineering. vol. 4960, pp. 76-85, PROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Biomedical Optoacoutics IV, San Jose, CA, United States, 1/26/03. https://doi.org/10.1117/12.503617
Eghtedari MA, Copland JA, Popov V, Kotov NA, Motamedi M, Oraevsky AA. Bioconjugated gold nanoparticles as a contrast agent for optoacoustic detection of small tumors. In Oraevsky AA, editor, Proceedings of SPIE - The International Society for Optical Engineering. Vol. 4960. 2003. p. 76-85 https://doi.org/10.1117/12.503617
Eghtedari, Mohammad A. ; Copland, John A. ; Popov, Vsevolod ; Kotov, Nicholas A. ; Motamedi, Massoud ; Oraevsky, Alexander A. / Bioconjugated gold nanoparticles as a contrast agent for optoacoustic detection of small tumors. Proceedings of SPIE - The International Society for Optical Engineering. editor / A.A. Oraevsky. Vol. 4960 2003. pp. 76-85
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