Biological and antigenic characterization of Netivot virus, an unusual new Orbivirus recovered from mosquitoes in Israel

R. B. Tesh, J. Peleg, I. Samina, J. Margalit, D. K. Bodkin, R. E. Shope, D. Knudson

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The antigenic and biological characteristics of a new Orbivirus, designated Netivot virus, are described. This agent was originally recovered in cultures of the C6/36 clone of Aedes albopictus cells from a pool of Culex pipiens captured in Israel. Netivot virus is not pathogenic for newborn mice, nor did it initially produce detectable cytopathic effect (CPE) in Vero cells. It is closely related antigenically to Umatilla and Llano Seco viruses; these 3 agents appear to constitute a new serogroup within the genus Orbivirus. Netivot virus is also more distantly related to a number of other orbiviruses in the blue-tongue, epizootic hemorrhagic disease of deer, and Eubenangee serogroups. Netivot virus replicated to high titer and produced CPE in a variety of mosquito cell cultures, but it did not grow in 2 sand fly cell lines. Inoculation of Ae. aegypti and Ae. albopictus with Netivot virus resulted in almost 100% mortality in both species within 15 days after infection. The recovery of this and a number of other yet unidentified viral agents from field-collected mosquitoes in cultures of C6/36 cells, but not in the conventional vertebrate assay systems, suggests the existence in nature of many yet unrecognized mosquito-associated viruses. It also demonstrates the value of using new isolation methods in arbovirus studies.

Original languageEnglish (US)
Pages (from-to)418-428
Number of pages11
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume35
Issue number2
StatePublished - 1986
Externally publishedYes

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Orbivirus
Israel
Culicidae
Viruses
Bluetongue
Satellite Viruses
Arboviruses
Psychodidae
Culex
Vero Cells
Deer
Aedes
Vertebrates
Clone Cells
Cell Culture Techniques
Cell Line
Mortality
Infection

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Tesh, R. B., Peleg, J., Samina, I., Margalit, J., Bodkin, D. K., Shope, R. E., & Knudson, D. (1986). Biological and antigenic characterization of Netivot virus, an unusual new Orbivirus recovered from mosquitoes in Israel. American Journal of Tropical Medicine and Hygiene, 35(2), 418-428.

Biological and antigenic characterization of Netivot virus, an unusual new Orbivirus recovered from mosquitoes in Israel. / Tesh, R. B.; Peleg, J.; Samina, I.; Margalit, J.; Bodkin, D. K.; Shope, R. E.; Knudson, D.

In: American Journal of Tropical Medicine and Hygiene, Vol. 35, No. 2, 1986, p. 418-428.

Research output: Contribution to journalArticle

Tesh, RB, Peleg, J, Samina, I, Margalit, J, Bodkin, DK, Shope, RE & Knudson, D 1986, 'Biological and antigenic characterization of Netivot virus, an unusual new Orbivirus recovered from mosquitoes in Israel', American Journal of Tropical Medicine and Hygiene, vol. 35, no. 2, pp. 418-428.
Tesh, R. B. ; Peleg, J. ; Samina, I. ; Margalit, J. ; Bodkin, D. K. ; Shope, R. E. ; Knudson, D. / Biological and antigenic characterization of Netivot virus, an unusual new Orbivirus recovered from mosquitoes in Israel. In: American Journal of Tropical Medicine and Hygiene. 1986 ; Vol. 35, No. 2. pp. 418-428.
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