Biological and phylogenetic characterization of pigeon paramyxovirus serotype 1 circulating in wild North American pigeons and doves

L. Mia Kim, Daniel J. King, Hilda Guzman, Robert B. Tesh, Amelia P A Travassos Da Rosa, Rudy Bueno, James A. Dennett, Claudio L. Afonso

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

As part of West Nile virus surveillance programs in Rhode Island and eastern Texas between 2000 and 2007, brain tissue was collected from 5,608 dead birds representing 21 avian orders found in public places or reported by homeowners. Fifteen Newcastle disease virus isolates were recovered only from birds of the order Columbiformes and were positively identified by the USDA-validated real-time reverse transcription-PCR assay targeting the matrix gene and more specifically as pigeon paramyxovirus serotype 1 (PPMV-1) by hemagglutinin inhibition with monoclonal antibodies. Based upon partial genomic sequencing and phylogenetic analysis, the newly isolated viruses represent a distinct sublineage within class II genotype VIb. All of the viruses (15/15) were classified as virulent based upon their fusion cleavage site motif ( 112RRKKRF117) and intracerebral pathogenicity indices of >0.7 (ranging from 0.98 to1.35); however, these viruses escaped detection by the fusion gene-based real-time PCR test for virulence. Modifications introduced to the probe site of the fusion gene-based assay allowed rapid virulence detection within this distinct sublineage.

Original languageEnglish (US)
Pages (from-to)3303-3310
Number of pages8
JournalJournal of Clinical Microbiology
Volume46
Issue number10
DOIs
StatePublished - Oct 2008

Fingerprint

Columbidae
Virulence
Gene Fusion
Viruses
Birds
Columbiformes
United States Department of Agriculture
Newcastle disease virus
West Nile virus
Gene Targeting
Reverse Transcription
Real-Time Polymerase Chain Reaction
Genotype
Monoclonal Antibodies
Polymerase Chain Reaction
Brain
Serogroup

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Kim, L. M., King, D. J., Guzman, H., Tesh, R. B., Travassos Da Rosa, A. P. A., Bueno, R., ... Afonso, C. L. (2008). Biological and phylogenetic characterization of pigeon paramyxovirus serotype 1 circulating in wild North American pigeons and doves. Journal of Clinical Microbiology, 46(10), 3303-3310. https://doi.org/10.1128/JCM.00644-08

Biological and phylogenetic characterization of pigeon paramyxovirus serotype 1 circulating in wild North American pigeons and doves. / Kim, L. Mia; King, Daniel J.; Guzman, Hilda; Tesh, Robert B.; Travassos Da Rosa, Amelia P A; Bueno, Rudy; Dennett, James A.; Afonso, Claudio L.

In: Journal of Clinical Microbiology, Vol. 46, No. 10, 10.2008, p. 3303-3310.

Research output: Contribution to journalArticle

Kim, LM, King, DJ, Guzman, H, Tesh, RB, Travassos Da Rosa, APA, Bueno, R, Dennett, JA & Afonso, CL 2008, 'Biological and phylogenetic characterization of pigeon paramyxovirus serotype 1 circulating in wild North American pigeons and doves', Journal of Clinical Microbiology, vol. 46, no. 10, pp. 3303-3310. https://doi.org/10.1128/JCM.00644-08
Kim, L. Mia ; King, Daniel J. ; Guzman, Hilda ; Tesh, Robert B. ; Travassos Da Rosa, Amelia P A ; Bueno, Rudy ; Dennett, James A. ; Afonso, Claudio L. / Biological and phylogenetic characterization of pigeon paramyxovirus serotype 1 circulating in wild North American pigeons and doves. In: Journal of Clinical Microbiology. 2008 ; Vol. 46, No. 10. pp. 3303-3310.
@article{b660dceff3a0419b950936671adbcc82,
title = "Biological and phylogenetic characterization of pigeon paramyxovirus serotype 1 circulating in wild North American pigeons and doves",
abstract = "As part of West Nile virus surveillance programs in Rhode Island and eastern Texas between 2000 and 2007, brain tissue was collected from 5,608 dead birds representing 21 avian orders found in public places or reported by homeowners. Fifteen Newcastle disease virus isolates were recovered only from birds of the order Columbiformes and were positively identified by the USDA-validated real-time reverse transcription-PCR assay targeting the matrix gene and more specifically as pigeon paramyxovirus serotype 1 (PPMV-1) by hemagglutinin inhibition with monoclonal antibodies. Based upon partial genomic sequencing and phylogenetic analysis, the newly isolated viruses represent a distinct sublineage within class II genotype VIb. All of the viruses (15/15) were classified as virulent based upon their fusion cleavage site motif ( 112RRKKRF117) and intracerebral pathogenicity indices of >0.7 (ranging from 0.98 to1.35); however, these viruses escaped detection by the fusion gene-based real-time PCR test for virulence. Modifications introduced to the probe site of the fusion gene-based assay allowed rapid virulence detection within this distinct sublineage.",
author = "Kim, {L. Mia} and King, {Daniel J.} and Hilda Guzman and Tesh, {Robert B.} and {Travassos Da Rosa}, {Amelia P A} and Rudy Bueno and Dennett, {James A.} and Afonso, {Claudio L.}",
year = "2008",
month = "10",
doi = "10.1128/JCM.00644-08",
language = "English (US)",
volume = "46",
pages = "3303--3310",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "10",

}

TY - JOUR

T1 - Biological and phylogenetic characterization of pigeon paramyxovirus serotype 1 circulating in wild North American pigeons and doves

AU - Kim, L. Mia

AU - King, Daniel J.

AU - Guzman, Hilda

AU - Tesh, Robert B.

AU - Travassos Da Rosa, Amelia P A

AU - Bueno, Rudy

AU - Dennett, James A.

AU - Afonso, Claudio L.

PY - 2008/10

Y1 - 2008/10

N2 - As part of West Nile virus surveillance programs in Rhode Island and eastern Texas between 2000 and 2007, brain tissue was collected from 5,608 dead birds representing 21 avian orders found in public places or reported by homeowners. Fifteen Newcastle disease virus isolates were recovered only from birds of the order Columbiformes and were positively identified by the USDA-validated real-time reverse transcription-PCR assay targeting the matrix gene and more specifically as pigeon paramyxovirus serotype 1 (PPMV-1) by hemagglutinin inhibition with monoclonal antibodies. Based upon partial genomic sequencing and phylogenetic analysis, the newly isolated viruses represent a distinct sublineage within class II genotype VIb. All of the viruses (15/15) were classified as virulent based upon their fusion cleavage site motif ( 112RRKKRF117) and intracerebral pathogenicity indices of >0.7 (ranging from 0.98 to1.35); however, these viruses escaped detection by the fusion gene-based real-time PCR test for virulence. Modifications introduced to the probe site of the fusion gene-based assay allowed rapid virulence detection within this distinct sublineage.

AB - As part of West Nile virus surveillance programs in Rhode Island and eastern Texas between 2000 and 2007, brain tissue was collected from 5,608 dead birds representing 21 avian orders found in public places or reported by homeowners. Fifteen Newcastle disease virus isolates were recovered only from birds of the order Columbiformes and were positively identified by the USDA-validated real-time reverse transcription-PCR assay targeting the matrix gene and more specifically as pigeon paramyxovirus serotype 1 (PPMV-1) by hemagglutinin inhibition with monoclonal antibodies. Based upon partial genomic sequencing and phylogenetic analysis, the newly isolated viruses represent a distinct sublineage within class II genotype VIb. All of the viruses (15/15) were classified as virulent based upon their fusion cleavage site motif ( 112RRKKRF117) and intracerebral pathogenicity indices of >0.7 (ranging from 0.98 to1.35); however, these viruses escaped detection by the fusion gene-based real-time PCR test for virulence. Modifications introduced to the probe site of the fusion gene-based assay allowed rapid virulence detection within this distinct sublineage.

UR - http://www.scopus.com/inward/record.url?scp=53649086953&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=53649086953&partnerID=8YFLogxK

U2 - 10.1128/JCM.00644-08

DO - 10.1128/JCM.00644-08

M3 - Article

C2 - 18716227

AN - SCOPUS:53649086953

VL - 46

SP - 3303

EP - 3310

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 10

ER -