Abstract
The selenocyanate anion, SeCN-, has been reported in wastewater from refineries whose petroleum comes from Se-rich marine shales. A metalloid-resistant bacterium was exposed to aqueous solutions of SeCN - to examine the relative toxicity of SeCN-, and the results were compared with the toxicity of selenate and selenite and another G16 metalloid oxyanion, tellurite. We also determined the volatile organo-selenium species produced by bacterial cultures amended with selenocyanate anion, and we investigated a solid phase preconcentration technique for collecting SeCN - from aqueous samples with different ionic strengths and subsequent detection using capillary electrophoresis. The relative toxicity of SeCN - is comparable to that of selenate and selenite using the metalloid-resistant bacterium LHVE as the test organism. Tellurite was more toxic at all concentrations examined than all three selenium-containing anions, SeO4 2-, SeO3 2-, SeCN-. Live cultures of LHVE amended with 1 mM NaSeCN produced volatile organo-sulphides and organo-selenides that could be collected in headspace using a solid phase microextraction fibre. The bioprocessing, i.e. the reduction and methylation of SeCN-, is similar to that of selenate and selenite by other metalloid-resistant bacteria. An aqueous 1.0 mM solution of SeCN - could be captured from solution on solid-phase extraction (SPE) cartridges using an aminopropyl-based stationary phase. Selenocyanate anions, slowly pumped into a wetted SPE cartridge, were trapped on the cartridge's solid phase and were subsequently eluted, thereby providing an increase in concentration above that of the original SeCN- containing solution. Preconcentration factors of 3.9 were achieved using a mixed sodium hydroxide/methanol elution solvent and by adding NaCl to aqueous SeCN- before loading on the SPE cartridge.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1327-1335 |
| Number of pages | 9 |
| Journal | Environmental Technology |
| Volume | 30 |
| Issue number | 12 |
| DOIs | |
| State | Published - Nov 2009 |
| Externally published | Yes |
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Keywords
- Environmental selenium
- Petrochemical wastewater
- SeCN
- Solid phase extraction of metalloid anions
- Specific growth rate
ASJC Scopus subject areas
- Environmental Chemistry
- Waste Management and Disposal
- Water Science and Technology
Cite this
Biological interactions of selenocyanate : Bioprocessing, detection and toxicity. / Burra, Radhika; Fox, James D.; Pradenas, Gonzalo A.; Vásquez, Claudio C.; Chasteen, Thomas G.
In: Environmental Technology, Vol. 30, No. 12, 11.2009, p. 1327-1335.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Biological interactions of selenocyanate
T2 - Bioprocessing, detection and toxicity
AU - Burra, Radhika
AU - Fox, James D.
AU - Pradenas, Gonzalo A.
AU - Vásquez, Claudio C.
AU - Chasteen, Thomas G.
PY - 2009/11
Y1 - 2009/11
N2 - The selenocyanate anion, SeCN-, has been reported in wastewater from refineries whose petroleum comes from Se-rich marine shales. A metalloid-resistant bacterium was exposed to aqueous solutions of SeCN - to examine the relative toxicity of SeCN-, and the results were compared with the toxicity of selenate and selenite and another G16 metalloid oxyanion, tellurite. We also determined the volatile organo-selenium species produced by bacterial cultures amended with selenocyanate anion, and we investigated a solid phase preconcentration technique for collecting SeCN - from aqueous samples with different ionic strengths and subsequent detection using capillary electrophoresis. The relative toxicity of SeCN - is comparable to that of selenate and selenite using the metalloid-resistant bacterium LHVE as the test organism. Tellurite was more toxic at all concentrations examined than all three selenium-containing anions, SeO4 2-, SeO3 2-, SeCN-. Live cultures of LHVE amended with 1 mM NaSeCN produced volatile organo-sulphides and organo-selenides that could be collected in headspace using a solid phase microextraction fibre. The bioprocessing, i.e. the reduction and methylation of SeCN-, is similar to that of selenate and selenite by other metalloid-resistant bacteria. An aqueous 1.0 mM solution of SeCN - could be captured from solution on solid-phase extraction (SPE) cartridges using an aminopropyl-based stationary phase. Selenocyanate anions, slowly pumped into a wetted SPE cartridge, were trapped on the cartridge's solid phase and were subsequently eluted, thereby providing an increase in concentration above that of the original SeCN- containing solution. Preconcentration factors of 3.9 were achieved using a mixed sodium hydroxide/methanol elution solvent and by adding NaCl to aqueous SeCN- before loading on the SPE cartridge.
AB - The selenocyanate anion, SeCN-, has been reported in wastewater from refineries whose petroleum comes from Se-rich marine shales. A metalloid-resistant bacterium was exposed to aqueous solutions of SeCN - to examine the relative toxicity of SeCN-, and the results were compared with the toxicity of selenate and selenite and another G16 metalloid oxyanion, tellurite. We also determined the volatile organo-selenium species produced by bacterial cultures amended with selenocyanate anion, and we investigated a solid phase preconcentration technique for collecting SeCN - from aqueous samples with different ionic strengths and subsequent detection using capillary electrophoresis. The relative toxicity of SeCN - is comparable to that of selenate and selenite using the metalloid-resistant bacterium LHVE as the test organism. Tellurite was more toxic at all concentrations examined than all three selenium-containing anions, SeO4 2-, SeO3 2-, SeCN-. Live cultures of LHVE amended with 1 mM NaSeCN produced volatile organo-sulphides and organo-selenides that could be collected in headspace using a solid phase microextraction fibre. The bioprocessing, i.e. the reduction and methylation of SeCN-, is similar to that of selenate and selenite by other metalloid-resistant bacteria. An aqueous 1.0 mM solution of SeCN - could be captured from solution on solid-phase extraction (SPE) cartridges using an aminopropyl-based stationary phase. Selenocyanate anions, slowly pumped into a wetted SPE cartridge, were trapped on the cartridge's solid phase and were subsequently eluted, thereby providing an increase in concentration above that of the original SeCN- containing solution. Preconcentration factors of 3.9 were achieved using a mixed sodium hydroxide/methanol elution solvent and by adding NaCl to aqueous SeCN- before loading on the SPE cartridge.
KW - Environmental selenium
KW - Petrochemical wastewater
KW - SeCN
KW - Solid phase extraction of metalloid anions
KW - Specific growth rate
UR - http://www.scopus.com/inward/record.url?scp=70449109712&partnerID=8YFLogxK
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U2 - 10.1080/09593330902998082
DO - 10.1080/09593330902998082
M3 - Article
VL - 30
SP - 1327
EP - 1335
JO - Environmental Technology (United Kingdom)
JF - Environmental Technology (United Kingdom)
SN - 0959-3330
IS - 12
ER -