Biophysical characterization and vector-specific antagonist activity of domain III of the tick-borne flavivirus envelope protein

S. Bhardwaj, M. Holbrook, R. E. Shope, Alan Barrett, Stanley Watowich

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

The molecular determinants responsible for flavivirus host cell binding and tissue tropism are largely unknown, although domain III of the envelope protein has been implicated in these functions. We examined the solution properties and antagonist activity of Langat virus domain III. Our results suggest that domain III adopts a stably folded structure that can mediate binding of tick-borne flaviviruses but not mosquito-borne flaviviruses to their target cells. Three clusters of phylogenetically conserved residues are identified that may be responsible for the vector-specific antagonist activity of domain III.

Original languageEnglish (US)
Pages (from-to)4002-4007
Number of pages6
JournalJournal of Virology
Volume75
Issue number8
DOIs
StatePublished - 2001

Fingerprint

Flaviviridae
Flavivirus
Ticks
Viral Tropism
Langat virus
ticks
antagonists
tissue tropism
Tick-Borne Encephalitis Viruses
Culicidae
Proteins
proteins
cells

ASJC Scopus subject areas

  • Immunology

Cite this

Biophysical characterization and vector-specific antagonist activity of domain III of the tick-borne flavivirus envelope protein. / Bhardwaj, S.; Holbrook, M.; Shope, R. E.; Barrett, Alan; Watowich, Stanley.

In: Journal of Virology, Vol. 75, No. 8, 2001, p. 4002-4007.

Research output: Contribution to journalArticle

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