Biosynthesis of rabbit haptoglobin: Chemical evidence for a single chain precursor

Virginia Chow, Robert K. Murray, James D. Dixon, Alexander Kurosky

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    The primary translation product of the mRNA for rabbit haptoglobin was obtained from a rabbit reticulocyte lysate cell-free system by immunoprecipitation with an antiserum that was directed to the β chain of haptoglobin. Analysis of the translation product by gel electrophoresis and by protein sequencing analysis identified a single polypeptide of Mr 41 000. Sequence analysis established a signal region of 18 residues that was immediately followed by the α chain sequence. These results give strong evidence that haptoglobin is initially synthesized as a single chain composed of a signal peptide followed by α and β chain regions, respectively.

    Original languageEnglish (US)
    Pages (from-to)275-279
    Number of pages5
    JournalFEBS Letters
    Volume153
    Issue number2
    DOIs
    StatePublished - Mar 21 1983

    Fingerprint

    Haptoglobins
    Biosynthesis
    Rabbits
    Cell-Free System
    Reticulocytes
    Protein Sequence Analysis
    Protein Biosynthesis
    Protein Sorting Signals
    Electrophoresis
    Immunoprecipitation
    Sequence Analysis
    Immune Sera
    Gels
    Messenger RNA
    Peptides
    Proteins

    Keywords

    • Cell-free translation
    • Haptoglobin
    • Precursor protein
    • Signal sequence

    ASJC Scopus subject areas

    • Biochemistry
    • Biophysics
    • Molecular Biology

    Cite this

    Biosynthesis of rabbit haptoglobin : Chemical evidence for a single chain precursor. / Chow, Virginia; Murray, Robert K.; Dixon, James D.; Kurosky, Alexander.

    In: FEBS Letters, Vol. 153, No. 2, 21.03.1983, p. 275-279.

    Research output: Contribution to journalArticle

    Chow, Virginia ; Murray, Robert K. ; Dixon, James D. ; Kurosky, Alexander. / Biosynthesis of rabbit haptoglobin : Chemical evidence for a single chain precursor. In: FEBS Letters. 1983 ; Vol. 153, No. 2. pp. 275-279.
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