Biosynthesis of rabbit haptoglobin: Chemical evidence for a single chain precursor

Virginia Chow, Robert K. Murray, James D. Dixon, Alexander Kurosky

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


The primary translation product of the mRNA for rabbit haptoglobin was obtained from a rabbit reticulocyte lysate cell-free system by immunoprecipitation with an antiserum that was directed to the β chain of haptoglobin. Analysis of the translation product by gel electrophoresis and by protein sequencing analysis identified a single polypeptide of Mr 41 000. Sequence analysis established a signal region of 18 residues that was immediately followed by the α chain sequence. These results give strong evidence that haptoglobin is initially synthesized as a single chain composed of a signal peptide followed by α and β chain regions, respectively.

Original languageEnglish (US)
Pages (from-to)275-279
Number of pages5
JournalFEBS Letters
Issue number2
StatePublished - Mar 21 1983
Externally publishedYes


  • Cell-free translation
  • Haptoglobin
  • Precursor protein
  • Signal sequence

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology


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