Biosynthesis of rabbit haptoglobin: Chemical evidence for a single chain precursor

Virginia Chow; Robert K. Murray; James D. Dixon; Alexander Kurosky

doi:10.1016/0014-5793(83)80623-1

Biosynthesis of rabbit haptoglobin: Chemical evidence for a single chain precursor

Virginia Chow, Robert K. Murray, James D. Dixon, Alexander Kurosky

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

The primary translation product of the mRNA for rabbit haptoglobin was obtained from a rabbit reticulocyte lysate cell-free system by immunoprecipitation with an antiserum that was directed to the β chain of haptoglobin. Analysis of the translation product by gel electrophoresis and by protein sequencing analysis identified a single polypeptide of M_r 41 000. Sequence analysis established a signal region of 18 residues that was immediately followed by the α chain sequence. These results give strong evidence that haptoglobin is initially synthesized as a single chain composed of a signal peptide followed by α and β chain regions, respectively.

Original language	English (US)
Pages (from-to)	275-279
Number of pages	5
Journal	FEBS Letters
Volume	153
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(83)80623-1
State	Published - Mar 21 1983
Externally published	Yes

Keywords

Cell-free translation
Haptoglobin
Precursor protein
Signal sequence

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(83)80623-1

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title = "Biosynthesis of rabbit haptoglobin: Chemical evidence for a single chain precursor",

abstract = "The primary translation product of the mRNA for rabbit haptoglobin was obtained from a rabbit reticulocyte lysate cell-free system by immunoprecipitation with an antiserum that was directed to the β chain of haptoglobin. Analysis of the translation product by gel electrophoresis and by protein sequencing analysis identified a single polypeptide of Mr 41 000. Sequence analysis established a signal region of 18 residues that was immediately followed by the α chain sequence. These results give strong evidence that haptoglobin is initially synthesized as a single chain composed of a signal peptide followed by α and β chain regions, respectively.",

keywords = "Cell-free translation, Haptoglobin, Precursor protein, Signal sequence",

author = "Virginia Chow and Murray, \{Robert K.\} and Dixon, \{James D.\} and Alexander Kurosky",

note = "Funding Information: We wish to thank Horace D. Kelso and Linda Merryman for excellentt echnicala ssistanceT. his work was supportedb y MRC of Canada,b y grant GM 29039f rom the National Instituteo f General Medical Sciencesa, nd by grantC A 17701fr om the National Cancer Institute.",

year = "1983",

month = mar,

day = "21",

doi = "10.1016/0014-5793(83)80623-1",

language = "English (US)",

volume = "153",

pages = "275--279",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc.",

number = "2",

}

TY - JOUR

T1 - Biosynthesis of rabbit haptoglobin

T2 - Chemical evidence for a single chain precursor

AU - Chow, Virginia

AU - Murray, Robert K.

AU - Dixon, James D.

AU - Kurosky, Alexander

N1 - Funding Information: We wish to thank Horace D. Kelso and Linda Merryman for excellentt echnicala ssistanceT. his work was supportedb y MRC of Canada,b y grant GM 29039f rom the National Instituteo f General Medical Sciencesa, nd by grantC A 17701fr om the National Cancer Institute.

PY - 1983/3/21

Y1 - 1983/3/21

N2 - The primary translation product of the mRNA for rabbit haptoglobin was obtained from a rabbit reticulocyte lysate cell-free system by immunoprecipitation with an antiserum that was directed to the β chain of haptoglobin. Analysis of the translation product by gel electrophoresis and by protein sequencing analysis identified a single polypeptide of Mr 41 000. Sequence analysis established a signal region of 18 residues that was immediately followed by the α chain sequence. These results give strong evidence that haptoglobin is initially synthesized as a single chain composed of a signal peptide followed by α and β chain regions, respectively.

AB - The primary translation product of the mRNA for rabbit haptoglobin was obtained from a rabbit reticulocyte lysate cell-free system by immunoprecipitation with an antiserum that was directed to the β chain of haptoglobin. Analysis of the translation product by gel electrophoresis and by protein sequencing analysis identified a single polypeptide of Mr 41 000. Sequence analysis established a signal region of 18 residues that was immediately followed by the α chain sequence. These results give strong evidence that haptoglobin is initially synthesized as a single chain composed of a signal peptide followed by α and β chain regions, respectively.

KW - Cell-free translation

KW - Haptoglobin

KW - Precursor protein

KW - Signal sequence

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U2 - 10.1016/0014-5793(83)80623-1

DO - 10.1016/0014-5793(83)80623-1

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AN - SCOPUS:0020575312

SN - 0014-5793

VL - 153

SP - 275

EP - 279

JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

Biosynthesis of rabbit haptoglobin: Chemical evidence for a single chain precursor

Abstract

Keywords

ASJC Scopus subject areas

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