Biotinylated peptides/proteins. II. Identification of biotinylated lysyl phenylthiohydantoins

John S. Smith, Brian T. Miller, Alexander Kurosky

    Research output: Contribution to journalArticle

    13 Citations (Scopus)

    Abstract

    The identification and characterization of biotinylated lysyl residues in a polypeptide chain by automated sequence analysis is described. An in depth analytical study was conducted for the delineation of Nε{lunate} modification of lysyl residues with N-hydroxysuccinimide esters of biotin and 6-aminohexanoic biotin. Confirmation of the structure of the phenylthiohydantoin derivatives of Nε{lunate} biotinylated lysine was achieved by mass spectrometry. The analytical study focused on the identification of biotinylated lysine-4 of neuropeptide Y which served as a model peptide for the analytical procedures detailed.

    Original languageEnglish (US)
    Pages (from-to)254-257
    Number of pages4
    JournalAnalytical Biochemistry
    Volume197
    Issue number1
    DOIs
    StatePublished - Aug 15 1991

    Fingerprint

    Phenylthiohydantoin
    Biotin
    Lysine
    Peptides
    Neuropeptide Y
    Mass spectrometry
    Sequence Analysis
    Mass Spectrometry
    Esters
    Proteins
    Derivatives

    ASJC Scopus subject areas

    • Biochemistry
    • Biophysics
    • Molecular Biology

    Cite this

    Biotinylated peptides/proteins. II. Identification of biotinylated lysyl phenylthiohydantoins. / Smith, John S.; Miller, Brian T.; Kurosky, Alexander.

    In: Analytical Biochemistry, Vol. 197, No. 1, 15.08.1991, p. 254-257.

    Research output: Contribution to journalArticle

    Smith, John S. ; Miller, Brian T. ; Kurosky, Alexander. / Biotinylated peptides/proteins. II. Identification of biotinylated lysyl phenylthiohydantoins. In: Analytical Biochemistry. 1991 ; Vol. 197, No. 1. pp. 254-257.
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