Biotinylated peptides/proteins. II. Identification of biotinylated lysyl phenylthiohydantoins

John S. Smith, Brian T. Miller, Alexander Kurosky

    Research output: Contribution to journalArticlepeer-review

    14 Scopus citations


    The identification and characterization of biotinylated lysyl residues in a polypeptide chain by automated sequence analysis is described. An in depth analytical study was conducted for the delineation of Nε{lunate} modification of lysyl residues with N-hydroxysuccinimide esters of biotin and 6-aminohexanoic biotin. Confirmation of the structure of the phenylthiohydantoin derivatives of Nε{lunate} biotinylated lysine was achieved by mass spectrometry. The analytical study focused on the identification of biotinylated lysine-4 of neuropeptide Y which served as a model peptide for the analytical procedures detailed.

    Original languageEnglish (US)
    Pages (from-to)254-257
    Number of pages4
    JournalAnalytical Biochemistry
    Issue number1
    StatePublished - Aug 15 1991

    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology


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