Biventricular differences in ß-adrenergic receptor signaling following burn injury

Ashley Guillory, Robert P. Clayton, Anesh Prasai, Amina El Ayadi, David Herndon, Celeste Finnerty

Research output: Contribution to journalArticle

Abstract

Burn injury detrimentally affects the myocardium, primarily due to over-activation of ß-adrenergic receptors (ß-AR). Autopsy reports from our institution reveal that patients often suffer from right ventricle (RV) failure. Since burn injury affects ß-AR signaling in the left ventricle (LV), we proposed that ß-AR signaling may also be altered in the RV. A rodent model with a scald burn of 60% of the total body surface area was used to test this hypothesis. Ventricles were isolated 7 days post-burn. We examined the expression of ß-ARs via Western blotting and the mRNA expression of downstream signaling proteins via qRT-PCR. Cyclic adenosine monophosphate (cAMP) production and protein kinase A (PKA) activity were measured in membrane and cytosolic fractions, respectively, using enzyme immunoassay kits. ß1-AR protein expression was significantly increased in the RV following burn injury compared to non-burned RV but not in the LV (p = 0.0022). In contrast, ß2-AR expression was unaltered among the groups while GQi expression was significantly higher in the LV post-burn (p = 0.023). B-arrestin-1 and G-protein coupled receptor kinase-2 mRNA expression were significantly increased in the left ventricle post-burn (p = 0.001, p<0.0001, respectively). cAMP production and PKA activity were significantly lower in the LV post-burn (p = 0.0063, 0.0042, respectively). These data indicate that burn injury affects the ß-AR signaling pathway in the RV independently of the LV. Additionally, non-canonical ß-AR signaling may be activated in the RV as cAMP production and PKA activity were unchanged despite changes in ß1-AR protein expression.

Original languageEnglish (US)
Article numbere0189527
JournalPLoS One
Volume12
Issue number12
DOIs
StatePublished - Dec 1 2017

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burns (injuries)
adrenergic receptors
Adrenergic Receptors
Heart Ventricles
cAMP-dependent protein kinase
cyclic AMP
Cyclic AMP-Dependent Protein Kinases
Cyclic AMP
Wounds and Injuries
protein synthesis
arrestins
Burns
G-Protein-Coupled Receptor Kinase 2
enzyme immunoassays
Arrestin
myocardium
Messenger RNA
Proteins
necropsy
surface area

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Biventricular differences in ß-adrenergic receptor signaling following burn injury. / Guillory, Ashley; Clayton, Robert P.; Prasai, Anesh; El Ayadi, Amina; Herndon, David; Finnerty, Celeste.

In: PLoS One, Vol. 12, No. 12, e0189527, 01.12.2017.

Research output: Contribution to journalArticle

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