Blastocyst implantation in the Chinese hamster (Cricetulus griseus)

T. N. Blankenship, R. L. Given, T. A. Parkening

    Research output: Contribution to journalArticle

    22 Citations (Scopus)

    Abstract

    Embryonic development of the Chinese hamster (Cricetulus griseus) was studied from the onset of implantation to the formation of the parietal yolk sac placenta. Implantation began on day 6 of pregnancy, when the embryo became fixed to the uterine luminal epithelium. At this time there was no zona pellucida, and microvilli of the trophoblast and uterine epithelium were closely apposed. Stromal cells immediately adjacent to the implantation chamber began to enlarge and accumulate glycogen. By day 7 the mural trophoblast penetrated the luminal epithelium in discrete areas. The trophoblast appeared to phagocytize uterine epithelial cells, although epithelium adjoining the points of penetration was normal. In other areas nascent apical protrusions from the uterine epithelium indented the surface of the trophoblast. The epiblast had enlarged and both visceral and parietal endoderm cells were present. The well-developed decidual cells were epithelioid and completely surrounded the implantation chamber. On day 8 the uterine epithelium had disappeared along the mural surface of the embryo. The embryonic cell mass was elongated and filled the yolk sac cavity. Reichert's membrane was well developed. The uterine epithelial basal lamina was largely disrupted, and the trophoblast was in direct contact with decidual cells. Primary and secondary giant trophoblast cells were present and in contact with extravasated maternal blood. The mural trophoblast formed channels in which blood cells were found in close proximity to Reichert's membrane. Decidual cells were in contact with capillary epithelium and in some cases formed part of the vessel wall. Structural changes occurring in the embryo and endometrium during implantation in the Chinese hamster are described for the first time in this report and are compared to those described for some other myomorph rodents.

    Original languageEnglish (US)
    Pages (from-to)137-157
    Number of pages21
    JournalAmerican Journal of Anatomy
    Volume187
    Issue number2
    DOIs
    StatePublished - 1990

    Fingerprint

    Trophoblasts
    Cricetulus
    Epithelium
    Yolk Sac
    Embryonic Structures
    Germ Layers
    Epithelioid Cells
    Zona Pellucida
    Endoderm
    Membranes
    Giant Cells
    Stromal Cells
    Microvilli
    Endometrium
    Glycogen
    Basement Membrane
    Placenta
    Embryonic Development
    Rodentia
    Blood Cells

    ASJC Scopus subject areas

    • Anatomy

    Cite this

    Blastocyst implantation in the Chinese hamster (Cricetulus griseus). / Blankenship, T. N.; Given, R. L.; Parkening, T. A.

    In: American Journal of Anatomy, Vol. 187, No. 2, 1990, p. 137-157.

    Research output: Contribution to journalArticle

    Blankenship, T. N. ; Given, R. L. ; Parkening, T. A. / Blastocyst implantation in the Chinese hamster (Cricetulus griseus). In: American Journal of Anatomy. 1990 ; Vol. 187, No. 2. pp. 137-157.
    @article{6533b9d863ce43e68b589ec2ff50a2bf,
    title = "Blastocyst implantation in the Chinese hamster (Cricetulus griseus)",
    abstract = "Embryonic development of the Chinese hamster (Cricetulus griseus) was studied from the onset of implantation to the formation of the parietal yolk sac placenta. Implantation began on day 6 of pregnancy, when the embryo became fixed to the uterine luminal epithelium. At this time there was no zona pellucida, and microvilli of the trophoblast and uterine epithelium were closely apposed. Stromal cells immediately adjacent to the implantation chamber began to enlarge and accumulate glycogen. By day 7 the mural trophoblast penetrated the luminal epithelium in discrete areas. The trophoblast appeared to phagocytize uterine epithelial cells, although epithelium adjoining the points of penetration was normal. In other areas nascent apical protrusions from the uterine epithelium indented the surface of the trophoblast. The epiblast had enlarged and both visceral and parietal endoderm cells were present. The well-developed decidual cells were epithelioid and completely surrounded the implantation chamber. On day 8 the uterine epithelium had disappeared along the mural surface of the embryo. The embryonic cell mass was elongated and filled the yolk sac cavity. Reichert's membrane was well developed. The uterine epithelial basal lamina was largely disrupted, and the trophoblast was in direct contact with decidual cells. Primary and secondary giant trophoblast cells were present and in contact with extravasated maternal blood. The mural trophoblast formed channels in which blood cells were found in close proximity to Reichert's membrane. Decidual cells were in contact with capillary epithelium and in some cases formed part of the vessel wall. Structural changes occurring in the embryo and endometrium during implantation in the Chinese hamster are described for the first time in this report and are compared to those described for some other myomorph rodents.",
    author = "Blankenship, {T. N.} and Given, {R. L.} and Parkening, {T. A.}",
    year = "1990",
    doi = "10.1002/aja.1001870203",
    language = "English (US)",
    volume = "187",
    pages = "137--157",
    journal = "Developmental Dynamics",
    issn = "1058-8388",
    publisher = "Wiley-Liss Inc.",
    number = "2",

    }

    TY - JOUR

    T1 - Blastocyst implantation in the Chinese hamster (Cricetulus griseus)

    AU - Blankenship, T. N.

    AU - Given, R. L.

    AU - Parkening, T. A.

    PY - 1990

    Y1 - 1990

    N2 - Embryonic development of the Chinese hamster (Cricetulus griseus) was studied from the onset of implantation to the formation of the parietal yolk sac placenta. Implantation began on day 6 of pregnancy, when the embryo became fixed to the uterine luminal epithelium. At this time there was no zona pellucida, and microvilli of the trophoblast and uterine epithelium were closely apposed. Stromal cells immediately adjacent to the implantation chamber began to enlarge and accumulate glycogen. By day 7 the mural trophoblast penetrated the luminal epithelium in discrete areas. The trophoblast appeared to phagocytize uterine epithelial cells, although epithelium adjoining the points of penetration was normal. In other areas nascent apical protrusions from the uterine epithelium indented the surface of the trophoblast. The epiblast had enlarged and both visceral and parietal endoderm cells were present. The well-developed decidual cells were epithelioid and completely surrounded the implantation chamber. On day 8 the uterine epithelium had disappeared along the mural surface of the embryo. The embryonic cell mass was elongated and filled the yolk sac cavity. Reichert's membrane was well developed. The uterine epithelial basal lamina was largely disrupted, and the trophoblast was in direct contact with decidual cells. Primary and secondary giant trophoblast cells were present and in contact with extravasated maternal blood. The mural trophoblast formed channels in which blood cells were found in close proximity to Reichert's membrane. Decidual cells were in contact with capillary epithelium and in some cases formed part of the vessel wall. Structural changes occurring in the embryo and endometrium during implantation in the Chinese hamster are described for the first time in this report and are compared to those described for some other myomorph rodents.

    AB - Embryonic development of the Chinese hamster (Cricetulus griseus) was studied from the onset of implantation to the formation of the parietal yolk sac placenta. Implantation began on day 6 of pregnancy, when the embryo became fixed to the uterine luminal epithelium. At this time there was no zona pellucida, and microvilli of the trophoblast and uterine epithelium were closely apposed. Stromal cells immediately adjacent to the implantation chamber began to enlarge and accumulate glycogen. By day 7 the mural trophoblast penetrated the luminal epithelium in discrete areas. The trophoblast appeared to phagocytize uterine epithelial cells, although epithelium adjoining the points of penetration was normal. In other areas nascent apical protrusions from the uterine epithelium indented the surface of the trophoblast. The epiblast had enlarged and both visceral and parietal endoderm cells were present. The well-developed decidual cells were epithelioid and completely surrounded the implantation chamber. On day 8 the uterine epithelium had disappeared along the mural surface of the embryo. The embryonic cell mass was elongated and filled the yolk sac cavity. Reichert's membrane was well developed. The uterine epithelial basal lamina was largely disrupted, and the trophoblast was in direct contact with decidual cells. Primary and secondary giant trophoblast cells were present and in contact with extravasated maternal blood. The mural trophoblast formed channels in which blood cells were found in close proximity to Reichert's membrane. Decidual cells were in contact with capillary epithelium and in some cases formed part of the vessel wall. Structural changes occurring in the embryo and endometrium during implantation in the Chinese hamster are described for the first time in this report and are compared to those described for some other myomorph rodents.

    UR - http://www.scopus.com/inward/record.url?scp=0025169908&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0025169908&partnerID=8YFLogxK

    U2 - 10.1002/aja.1001870203

    DO - 10.1002/aja.1001870203

    M3 - Article

    VL - 187

    SP - 137

    EP - 157

    JO - Developmental Dynamics

    JF - Developmental Dynamics

    SN - 1058-8388

    IS - 2

    ER -