Blocking effect of Puerarin on calcium channel in isolated guinea pig ventricular myocytes

You-Wen Qian, Zhi Li, Luoxiu Huang, Xiaoling Han, Jiating Sun, Hanqing Zhou, Zhongyu Liu

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Objective: To identify whether Puerarin (Puer) has blocking effects on L-type calcium channel. Methods: We used whole-cell recording of patch-clamp techniques to analyse calcium channel current in ventricular myocytes isolated from Langendorff guinea pig hearts by collagenase. Results: In control group, peak calcium channel current was decreased by 10.6% and 29.8% at 5, 10 min of recording time, respectively. For the same period, 0.1 mmol/L Puer reduced calcium current by 23.9% and 72.4%. 0.1 and 1 mmol/L Puer inhibited the amplitude of peak calcium channel current by 40.6% and 63.2% after perfusing for 10 min. Compared with control group in which 'rundown' phenomenon was observed, Puer also demonstrated its blocking effect on L-type channel. While washout of Puer with perfusing solution, calcium channel current went down further instead of recovery. Current-voltage (I-V) curves showed that the levels of calcium channel current were obviously decreased by Puer (both 0.1 mmo/L and 1 mmo/L) form - 40 mV to + 10 mV. Conclusion: Puer has blocking effect on L-type calcium channel in a concentration dependent manner.

Original languageEnglish (US)
Pages (from-to)787-789
Number of pages3
JournalChinese Medical Journal
Volume112
Issue number9
StatePublished - 1999
Externally publishedYes

Fingerprint

Calcium Channels
Muscle Cells
Guinea Pigs
L-Type Calcium Channels
Patch-Clamp Techniques
L Forms
Control Groups
Collagenases
puerarin
Calcium

Keywords

  • Calcium channel
  • Myocytes
  • Patch-clamp
  • Puerarin

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Qian, Y-W., Li, Z., Huang, L., Han, X., Sun, J., Zhou, H., & Liu, Z. (1999). Blocking effect of Puerarin on calcium channel in isolated guinea pig ventricular myocytes. Chinese Medical Journal, 112(9), 787-789.

Blocking effect of Puerarin on calcium channel in isolated guinea pig ventricular myocytes. / Qian, You-Wen; Li, Zhi; Huang, Luoxiu; Han, Xiaoling; Sun, Jiating; Zhou, Hanqing; Liu, Zhongyu.

In: Chinese Medical Journal, Vol. 112, No. 9, 1999, p. 787-789.

Research output: Contribution to journalArticle

Qian, Y-W, Li, Z, Huang, L, Han, X, Sun, J, Zhou, H & Liu, Z 1999, 'Blocking effect of Puerarin on calcium channel in isolated guinea pig ventricular myocytes', Chinese Medical Journal, vol. 112, no. 9, pp. 787-789.
Qian, You-Wen ; Li, Zhi ; Huang, Luoxiu ; Han, Xiaoling ; Sun, Jiating ; Zhou, Hanqing ; Liu, Zhongyu. / Blocking effect of Puerarin on calcium channel in isolated guinea pig ventricular myocytes. In: Chinese Medical Journal. 1999 ; Vol. 112, No. 9. pp. 787-789.
@article{24f5eec9f6ad48dc82da0b9899d641bb,
title = "Blocking effect of Puerarin on calcium channel in isolated guinea pig ventricular myocytes",
abstract = "Objective: To identify whether Puerarin (Puer) has blocking effects on L-type calcium channel. Methods: We used whole-cell recording of patch-clamp techniques to analyse calcium channel current in ventricular myocytes isolated from Langendorff guinea pig hearts by collagenase. Results: In control group, peak calcium channel current was decreased by 10.6{\%} and 29.8{\%} at 5, 10 min of recording time, respectively. For the same period, 0.1 mmol/L Puer reduced calcium current by 23.9{\%} and 72.4{\%}. 0.1 and 1 mmol/L Puer inhibited the amplitude of peak calcium channel current by 40.6{\%} and 63.2{\%} after perfusing for 10 min. Compared with control group in which 'rundown' phenomenon was observed, Puer also demonstrated its blocking effect on L-type channel. While washout of Puer with perfusing solution, calcium channel current went down further instead of recovery. Current-voltage (I-V) curves showed that the levels of calcium channel current were obviously decreased by Puer (both 0.1 mmo/L and 1 mmo/L) form - 40 mV to + 10 mV. Conclusion: Puer has blocking effect on L-type calcium channel in a concentration dependent manner.",
keywords = "Calcium channel, Myocytes, Patch-clamp, Puerarin",
author = "You-Wen Qian and Zhi Li and Luoxiu Huang and Xiaoling Han and Jiating Sun and Hanqing Zhou and Zhongyu Liu",
year = "1999",
language = "English (US)",
volume = "112",
pages = "787--789",
journal = "Chinese Medical Journal",
issn = "0366-6999",
publisher = "Chinese Medical Association",
number = "9",

}

TY - JOUR

T1 - Blocking effect of Puerarin on calcium channel in isolated guinea pig ventricular myocytes

AU - Qian, You-Wen

AU - Li, Zhi

AU - Huang, Luoxiu

AU - Han, Xiaoling

AU - Sun, Jiating

AU - Zhou, Hanqing

AU - Liu, Zhongyu

PY - 1999

Y1 - 1999

N2 - Objective: To identify whether Puerarin (Puer) has blocking effects on L-type calcium channel. Methods: We used whole-cell recording of patch-clamp techniques to analyse calcium channel current in ventricular myocytes isolated from Langendorff guinea pig hearts by collagenase. Results: In control group, peak calcium channel current was decreased by 10.6% and 29.8% at 5, 10 min of recording time, respectively. For the same period, 0.1 mmol/L Puer reduced calcium current by 23.9% and 72.4%. 0.1 and 1 mmol/L Puer inhibited the amplitude of peak calcium channel current by 40.6% and 63.2% after perfusing for 10 min. Compared with control group in which 'rundown' phenomenon was observed, Puer also demonstrated its blocking effect on L-type channel. While washout of Puer with perfusing solution, calcium channel current went down further instead of recovery. Current-voltage (I-V) curves showed that the levels of calcium channel current were obviously decreased by Puer (both 0.1 mmo/L and 1 mmo/L) form - 40 mV to + 10 mV. Conclusion: Puer has blocking effect on L-type calcium channel in a concentration dependent manner.

AB - Objective: To identify whether Puerarin (Puer) has blocking effects on L-type calcium channel. Methods: We used whole-cell recording of patch-clamp techniques to analyse calcium channel current in ventricular myocytes isolated from Langendorff guinea pig hearts by collagenase. Results: In control group, peak calcium channel current was decreased by 10.6% and 29.8% at 5, 10 min of recording time, respectively. For the same period, 0.1 mmol/L Puer reduced calcium current by 23.9% and 72.4%. 0.1 and 1 mmol/L Puer inhibited the amplitude of peak calcium channel current by 40.6% and 63.2% after perfusing for 10 min. Compared with control group in which 'rundown' phenomenon was observed, Puer also demonstrated its blocking effect on L-type channel. While washout of Puer with perfusing solution, calcium channel current went down further instead of recovery. Current-voltage (I-V) curves showed that the levels of calcium channel current were obviously decreased by Puer (both 0.1 mmo/L and 1 mmo/L) form - 40 mV to + 10 mV. Conclusion: Puer has blocking effect on L-type calcium channel in a concentration dependent manner.

KW - Calcium channel

KW - Myocytes

KW - Patch-clamp

KW - Puerarin

UR - http://www.scopus.com/inward/record.url?scp=0032864998&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032864998&partnerID=8YFLogxK

M3 - Article

C2 - 11717945

AN - SCOPUS:0032864998

VL - 112

SP - 787

EP - 789

JO - Chinese Medical Journal

JF - Chinese Medical Journal

SN - 0366-6999

IS - 9

ER -