BoHV-4-based vector delivering Ebola virus surface glycoprotein

Alfonso Rosamilia, Sarah Jacca, Giulia Tebaldi, Silvia Tiberti, Valentina Franceschi, Francesca Macchi, Sandro Cavirani, Gary Kobinger, Donald P. Knowles, Gaetano Donofrio

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Background: Ebola virus (EBOV) is a Category A pathogen that is a member of Filoviridae family that causes hemorrhagic fever in humans and non-human primates. Unpredictable and devastating outbreaks of disease have recently occurred in Africa and current immunoprophylaxis and therapies are limited. The main limitation of working with pathogens like EBOV is the need for costly containment. To potentiate further and wider opportunity for EBOV prophylactics and therapies development, innovative approaches are necessary. Methods: In the present study, an antigen delivery platform based on a recombinant bovine herpesvirus 4 (BoHV-4), delivering a synthetic EBOV glycoprotein (GP) gene sequence, BoHV-4-syEBOVgD106TK, was generated. Results: EBOV GP was abundantly expressed by BoHV-4-syEBOVgD106TK transduced cells without decreasing viral replication. BoHV-4-syEBOVgD106TK immunized goats produced high titers of anti-EBOV GP antibodies and conferred a long lasting (up to 6 months), detectable antibody response. Furthermore, no evidence of BoHV-4-syEBOVgD106TK viremia and secondary localization was detected in any of the immunized animals. Conclusions: The BoHV-4-based vector approach described here, represents: an alternative antigen delivery system for vaccination and a proof of principle study for anti-EBOV antibodies generation in goats for potential immunotherapy applications.

Original languageEnglish (US)
Article number325
JournalJournal of Translational Medicine
Volume14
Issue number1
DOIs
StatePublished - Nov 24 2016
Externally publishedYes

Keywords

  • Bovine herpesvirus 4
  • Ebola virus
  • Recombineering
  • Vaccine platform
  • Viral vector

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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