Bombesin: An activator of specific Aeromonas antibody secretion in rat intestine

Gui Fang Jin, Yan Shi Guo, Clifford W. Houston

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The effect of bombesin (BBS) in modulating the secretion of specific Aeromonas antibodies in rat intestine was determined. Rats were immunized with the culture supernatant of Aeromonas hydrophila, isolate SSU. This culture supernatant contained a number of toxins that may be considered virulence factors. After 24 days of immunization, rats were anesthetized and a 10-cm intestinal segment was perfused with phosphate-buffered saline. The effluents were collected for measurement of IgA and IgG by the enzyme-linked immunosorbent assay. When compared with the effect of intravenous administration of normal saline in the control group, intravenous injection of BBS (20 μg/kg) in the experimental group caused a significant increase in rat intestinal IgA and IgG in perfusates. The stimulatory effects of BBS on the presence of IgA and IgG were depressed partially by proglumide, a receptor antagonist of cholecystokinin (CCK) and gastrin. Treatment with pentagastrin (250 μg/kg) accelerated intestinal secretion of IgA, but failed to stimulate intestinal IgG secretion. In addition, intravenous injection of CCK-8 (120 ng/kg) evoked the intestinal secretion of either IgA or IgG. These findings demonstrated that BBS, gastrin, and CCK can stimulate antibody secretion in rat intestine and the stimulatory effect of BBS may be mediated partially via release of CCK and gastrin. These results suggest that neuropeptides such as BBS and gastrointestinal hormones, eg, CCK and gastrin, may participate in the regulation of intestinal secretion of IgA and IgG antibodies, respectively, in rats.

Original languageEnglish (US)
Pages (from-to)1708-1712
Number of pages5
JournalDigestive Diseases and Sciences
Volume34
Issue number11
DOIs
StatePublished - Nov 1989

Fingerprint

Aeromonas
Bombesin
Immunoglobulin A
Intestinal Secretions
Intestines
Immunoglobulin G
Antibodies
Cholecystokinin
Gastrins
Intravenous Injections
Proglumide
Cholecystokinin B Receptor
Cholecystokinin Receptors
Gastrointestinal Hormones
Aeromonas hydrophila
Pentagastrin
Virulence Factors
Neuropeptides
Intravenous Administration
Immunization

Keywords

  • Aeromonas hydrophila
  • bombesin
  • cholecystokinin
  • gastrin
  • immunoglobulin A
  • immunoglobulin G
  • intestine
  • neuropeptides

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Bombesin : An activator of specific Aeromonas antibody secretion in rat intestine. / Jin, Gui Fang; Guo, Yan Shi; Houston, Clifford W.

In: Digestive Diseases and Sciences, Vol. 34, No. 11, 11.1989, p. 1708-1712.

Research output: Contribution to journalArticle

Jin, Gui Fang ; Guo, Yan Shi ; Houston, Clifford W. / Bombesin : An activator of specific Aeromonas antibody secretion in rat intestine. In: Digestive Diseases and Sciences. 1989 ; Vol. 34, No. 11. pp. 1708-1712.
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