Bombesin inhibits growth of human pancreatic adenocarcinoma in nude mice

R. W. Alexander, J. R. Upp, G. J. Poston, Courtney Townsend, Pomila Singh, J. C. Thompson

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Bombesin, a 14-amino acid peptide, exhibits direct and indirect effects on the gastrointestinal tract, including release of hormones, stimulation of pancreatic, gastric, and intestinal secretion and intestinal motility. Cholecystokinin (CCK) and gastrin, two of the hormones released by bombesin, have been shown to play a role in maintaining the growth of normal gastrointestinal mucosa as well as in eliciting trophic responses in normal and neoplastic tissue. We studied the effects of chronic bombesin treatment on the growth of a human ductal pancreatic adenocarcinoma (SKI) xenografted into nude mice, and on the growth of the normal nude mouse pancreas. Thirteen nude mice were implanted with SKI tumor and divided into two groups. Mice received 0.1 ml intraperitoneal injections of either bombesin (20 μg/kg) or the vehicle alone three times per day. Tumor areas were measured twice weekly until death (week 8), at which time the tumors and the host pancreas were excised, weighed, and assayed for protein, RNA, and DNA content. Significant inhibition of tumor growth was found in the bombesin-treated group at weeks 4, 5, 6, 7, and 8. Tumor area and weight at death (day 57) were significantly less in the bombesin-treated group (48 and 46%) as compared with control. We observed similar inhibition of tumor DNA (39%), RNA (38%), and protein (43%) content compared with controls. In contrast, bombesin significantly increased the weight (64%), protein (81%), and DNA (73%) content of the mouse pancreas compared with controls. We conclude that bombesin acts concurrently as both a trophic agent for normal host pancreas and a growth inhibitory agent in xenografted pancreatic cancer tissue.

Original languageEnglish (US)
Pages (from-to)297-302
Number of pages6
JournalPancreas
Volume3
Issue number3
StatePublished - 1988

Fingerprint

Bombesin
Nude Mice
Adenocarcinoma
Growth
Pancreas
Neoplasms
DNA
Pancreatic Hormones
RNA
Intestinal Secretions
Proteins
Gastrointestinal Motility
Cholecystokinin
Gastrins
Tumor Burden
Intraperitoneal Injections
Pancreatic Neoplasms
Gastrointestinal Tract
Stomach
Mucous Membrane

ASJC Scopus subject areas

  • Endocrinology
  • Gastroenterology

Cite this

Alexander, R. W., Upp, J. R., Poston, G. J., Townsend, C., Singh, P., & Thompson, J. C. (1988). Bombesin inhibits growth of human pancreatic adenocarcinoma in nude mice. Pancreas, 3(3), 297-302.

Bombesin inhibits growth of human pancreatic adenocarcinoma in nude mice. / Alexander, R. W.; Upp, J. R.; Poston, G. J.; Townsend, Courtney; Singh, Pomila; Thompson, J. C.

In: Pancreas, Vol. 3, No. 3, 1988, p. 297-302.

Research output: Contribution to journalArticle

Alexander, RW, Upp, JR, Poston, GJ, Townsend, C, Singh, P & Thompson, JC 1988, 'Bombesin inhibits growth of human pancreatic adenocarcinoma in nude mice', Pancreas, vol. 3, no. 3, pp. 297-302.
Alexander RW, Upp JR, Poston GJ, Townsend C, Singh P, Thompson JC. Bombesin inhibits growth of human pancreatic adenocarcinoma in nude mice. Pancreas. 1988;3(3):297-302.
Alexander, R. W. ; Upp, J. R. ; Poston, G. J. ; Townsend, Courtney ; Singh, Pomila ; Thompson, J. C. / Bombesin inhibits growth of human pancreatic adenocarcinoma in nude mice. In: Pancreas. 1988 ; Vol. 3, No. 3. pp. 297-302.
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