Bombesin inhibits growth of pancreatic ductal adenocarcinoma (H2T) in nude mice

A. Farre, J. Ishizuka, Guillermo Gomez, B. M. Evers, R. Saydjari, Young Koo Ja Young Koo, Courtney Townsend, J. C. Thompson

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Bombesin (BBS), a tetradecapeptide, stimulates growth of various types of cells, including fibroblasts and human small cell lung cancer, and has been termed the universal 'on-switch' due to its ability to stimulate the release of numerous hormones. In addition, BBS receptors have been identified in normal and neoplastic pancreatic tissue. A pancreatic ductal adenocarcinoma cell line (H2T), established in our laboratory, possess specific binding sites for BBS. The purpose of this study was to examine the effect of BBS on the growth of H2T tumors transplanted into athymic nude mice. H2T cells (5 x 106 cells/mouse) were injected s.c. into the interscapular region of the nude mice and then the mice were randomized into two groups (n = 10/group). Mice received either 0.1 ml of saline with 0.1% bovine serum albumin (BSA) (control) or 0.1 ml BBS (5 μg/kg) intraperitoneally, three times/day. Tumor area was measured twice weekly until the mice were killed (day 32), when tumor and normal pancreas were removed, weighed, and assayed for DNA and protein content. Administration of BBS significantly inhibited H2T tumor area, weight, and DNA and protein content. Conversely, growth of normal pancreas, removed as an in vivo bioassay so as to ensure the efficacy of BBS, was stimulated. We conclude that BBS is a growth inhibitory factor for H2T tumors and that different mechanisms may be responsible for the differential growth effects elicited by normal and neoplastic pancreas in response to BBS.

Original languageEnglish (US)
Pages (from-to)652-656
Number of pages5
JournalPancreas
Volume9
Issue number5
StatePublished - 1994

Fingerprint

Bombesin
Nude Mice
Adenocarcinoma
Growth
Pancreas
Neoplasms
Bombesin Receptors
DNA
Small Cell Lung Carcinoma
Bovine Serum Albumin
Tumor Burden
Biological Assay
Proteins
Fibroblasts
Binding Sites
Hormones
Cell Line

Keywords

  • Bombesin
  • Pancreatic cancer
  • Pancreatic growth

ASJC Scopus subject areas

  • Endocrinology
  • Gastroenterology

Cite this

Farre, A., Ishizuka, J., Gomez, G., Evers, B. M., Saydjari, R., Ja Young Koo, Y. K., ... Thompson, J. C. (1994). Bombesin inhibits growth of pancreatic ductal adenocarcinoma (H2T) in nude mice. Pancreas, 9(5), 652-656.

Bombesin inhibits growth of pancreatic ductal adenocarcinoma (H2T) in nude mice. / Farre, A.; Ishizuka, J.; Gomez, Guillermo; Evers, B. M.; Saydjari, R.; Ja Young Koo, Young Koo; Townsend, Courtney; Thompson, J. C.

In: Pancreas, Vol. 9, No. 5, 1994, p. 652-656.

Research output: Contribution to journalArticle

Farre, A, Ishizuka, J, Gomez, G, Evers, BM, Saydjari, R, Ja Young Koo, YK, Townsend, C & Thompson, JC 1994, 'Bombesin inhibits growth of pancreatic ductal adenocarcinoma (H2T) in nude mice', Pancreas, vol. 9, no. 5, pp. 652-656.
Farre A, Ishizuka J, Gomez G, Evers BM, Saydjari R, Ja Young Koo YK et al. Bombesin inhibits growth of pancreatic ductal adenocarcinoma (H2T) in nude mice. Pancreas. 1994;9(5):652-656.
Farre, A. ; Ishizuka, J. ; Gomez, Guillermo ; Evers, B. M. ; Saydjari, R. ; Ja Young Koo, Young Koo ; Townsend, Courtney ; Thompson, J. C. / Bombesin inhibits growth of pancreatic ductal adenocarcinoma (H2T) in nude mice. In: Pancreas. 1994 ; Vol. 9, No. 5. pp. 652-656.
@article{6a0842e007894904b044983337f00c22,
title = "Bombesin inhibits growth of pancreatic ductal adenocarcinoma (H2T) in nude mice",
abstract = "Bombesin (BBS), a tetradecapeptide, stimulates growth of various types of cells, including fibroblasts and human small cell lung cancer, and has been termed the universal 'on-switch' due to its ability to stimulate the release of numerous hormones. In addition, BBS receptors have been identified in normal and neoplastic pancreatic tissue. A pancreatic ductal adenocarcinoma cell line (H2T), established in our laboratory, possess specific binding sites for BBS. The purpose of this study was to examine the effect of BBS on the growth of H2T tumors transplanted into athymic nude mice. H2T cells (5 x 106 cells/mouse) were injected s.c. into the interscapular region of the nude mice and then the mice were randomized into two groups (n = 10/group). Mice received either 0.1 ml of saline with 0.1{\%} bovine serum albumin (BSA) (control) or 0.1 ml BBS (5 μg/kg) intraperitoneally, three times/day. Tumor area was measured twice weekly until the mice were killed (day 32), when tumor and normal pancreas were removed, weighed, and assayed for DNA and protein content. Administration of BBS significantly inhibited H2T tumor area, weight, and DNA and protein content. Conversely, growth of normal pancreas, removed as an in vivo bioassay so as to ensure the efficacy of BBS, was stimulated. We conclude that BBS is a growth inhibitory factor for H2T tumors and that different mechanisms may be responsible for the differential growth effects elicited by normal and neoplastic pancreas in response to BBS.",
keywords = "Bombesin, Pancreatic cancer, Pancreatic growth",
author = "A. Farre and J. Ishizuka and Guillermo Gomez and Evers, {B. M.} and R. Saydjari and {Ja Young Koo}, {Young Koo} and Courtney Townsend and Thompson, {J. C.}",
year = "1994",
language = "English (US)",
volume = "9",
pages = "652--656",
journal = "Pancreas",
issn = "0885-3177",
publisher = "Lippincott Williams and Wilkins",
number = "5",

}

TY - JOUR

T1 - Bombesin inhibits growth of pancreatic ductal adenocarcinoma (H2T) in nude mice

AU - Farre, A.

AU - Ishizuka, J.

AU - Gomez, Guillermo

AU - Evers, B. M.

AU - Saydjari, R.

AU - Ja Young Koo, Young Koo

AU - Townsend, Courtney

AU - Thompson, J. C.

PY - 1994

Y1 - 1994

N2 - Bombesin (BBS), a tetradecapeptide, stimulates growth of various types of cells, including fibroblasts and human small cell lung cancer, and has been termed the universal 'on-switch' due to its ability to stimulate the release of numerous hormones. In addition, BBS receptors have been identified in normal and neoplastic pancreatic tissue. A pancreatic ductal adenocarcinoma cell line (H2T), established in our laboratory, possess specific binding sites for BBS. The purpose of this study was to examine the effect of BBS on the growth of H2T tumors transplanted into athymic nude mice. H2T cells (5 x 106 cells/mouse) were injected s.c. into the interscapular region of the nude mice and then the mice were randomized into two groups (n = 10/group). Mice received either 0.1 ml of saline with 0.1% bovine serum albumin (BSA) (control) or 0.1 ml BBS (5 μg/kg) intraperitoneally, three times/day. Tumor area was measured twice weekly until the mice were killed (day 32), when tumor and normal pancreas were removed, weighed, and assayed for DNA and protein content. Administration of BBS significantly inhibited H2T tumor area, weight, and DNA and protein content. Conversely, growth of normal pancreas, removed as an in vivo bioassay so as to ensure the efficacy of BBS, was stimulated. We conclude that BBS is a growth inhibitory factor for H2T tumors and that different mechanisms may be responsible for the differential growth effects elicited by normal and neoplastic pancreas in response to BBS.

AB - Bombesin (BBS), a tetradecapeptide, stimulates growth of various types of cells, including fibroblasts and human small cell lung cancer, and has been termed the universal 'on-switch' due to its ability to stimulate the release of numerous hormones. In addition, BBS receptors have been identified in normal and neoplastic pancreatic tissue. A pancreatic ductal adenocarcinoma cell line (H2T), established in our laboratory, possess specific binding sites for BBS. The purpose of this study was to examine the effect of BBS on the growth of H2T tumors transplanted into athymic nude mice. H2T cells (5 x 106 cells/mouse) were injected s.c. into the interscapular region of the nude mice and then the mice were randomized into two groups (n = 10/group). Mice received either 0.1 ml of saline with 0.1% bovine serum albumin (BSA) (control) or 0.1 ml BBS (5 μg/kg) intraperitoneally, three times/day. Tumor area was measured twice weekly until the mice were killed (day 32), when tumor and normal pancreas were removed, weighed, and assayed for DNA and protein content. Administration of BBS significantly inhibited H2T tumor area, weight, and DNA and protein content. Conversely, growth of normal pancreas, removed as an in vivo bioassay so as to ensure the efficacy of BBS, was stimulated. We conclude that BBS is a growth inhibitory factor for H2T tumors and that different mechanisms may be responsible for the differential growth effects elicited by normal and neoplastic pancreas in response to BBS.

KW - Bombesin

KW - Pancreatic cancer

KW - Pancreatic growth

UR - http://www.scopus.com/inward/record.url?scp=0028129603&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028129603&partnerID=8YFLogxK

M3 - Article

C2 - 7809021

AN - SCOPUS:0028129603

VL - 9

SP - 652

EP - 656

JO - Pancreas

JF - Pancreas

SN - 0885-3177

IS - 5

ER -