Bombesin stimulates growth of human gastrinoma

Dai H. Chung, B. Mark Evers, R. Daniel Beauchamp, J. Robert Upp, Srinivasan Rajaraman, Courtney Townsend, James C. Thompson

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Background. We have previously reported the first establishment and characterization of a functioning human gastrinoma (PT) xenograft. Bombesin, the equivalent of the mammalian gastrin-releasing peptide, has trophic effects on normal and neoplastic tissues of the gastrointestinal tract; the effects of gut hormones on the growth of gastrinoma are not known. The purpose of this study was twofold: (1) to determine the presence of various gut peptides in PT and (2) to determine the effect of bombesin on the growth of PT xenografts. Methods. PT tumors were examined for expression (mRNA and protein) of various gut peptides by Northern hybridization and immunohistochemistry. In addition, PT xenografts were implanted as 3 mm 2 pieces bilaterally subcutaneously in athymic nude mice. Mice were divided into two groups to receive either bombesin (5 μg/kg) or saline administered as intraperitoneal injections every 8 hours. Tumor area was measured twice weekly until mice were sacrificed (day 28), when tumor and normal pancreas were removed, weighed, and assayed for DNA and protein content. Results. Both mRNAs and peptides of gastrin and chromogranin A were present in PT tumors. Bombesin significantly stimulated growth of PT tumors from day 18 until mice were sacrificed (day 28). As expected, bombesin stimulated pancreatic growth. Conclusions. We have demonstrated for the first time that bombesin is a trophic hormone for gastrinoma. The unique cell line PT contains gastrin and chromogranin A and will be a useful model to define the biologic mechanisms controlling the growth of human gastrinomas.

Original languageEnglish (US)
Pages (from-to)1059-1065
Number of pages7
JournalSurgery
Volume112
Issue number6
StatePublished - 1992

Fingerprint

Gastrinoma
Bombesin
Growth
Heterografts
Chromogranin A
Gastrins
Neoplasms
Nude Mice
Peptides
Gastrin-Releasing Peptide
Messenger RNA
Intraperitoneal Injections
Growth Hormone
Gastrointestinal Tract
Pancreas
Proteins
Immunohistochemistry
Hormones
Cell Line
DNA

ASJC Scopus subject areas

  • Surgery

Cite this

Chung, D. H., Mark Evers, B., Daniel Beauchamp, R., Robert Upp, J., Rajaraman, S., Townsend, C., & Thompson, J. C. (1992). Bombesin stimulates growth of human gastrinoma. Surgery, 112(6), 1059-1065.

Bombesin stimulates growth of human gastrinoma. / Chung, Dai H.; Mark Evers, B.; Daniel Beauchamp, R.; Robert Upp, J.; Rajaraman, Srinivasan; Townsend, Courtney; Thompson, James C.

In: Surgery, Vol. 112, No. 6, 1992, p. 1059-1065.

Research output: Contribution to journalArticle

Chung, DH, Mark Evers, B, Daniel Beauchamp, R, Robert Upp, J, Rajaraman, S, Townsend, C & Thompson, JC 1992, 'Bombesin stimulates growth of human gastrinoma', Surgery, vol. 112, no. 6, pp. 1059-1065.
Chung DH, Mark Evers B, Daniel Beauchamp R, Robert Upp J, Rajaraman S, Townsend C et al. Bombesin stimulates growth of human gastrinoma. Surgery. 1992;112(6):1059-1065.
Chung, Dai H. ; Mark Evers, B. ; Daniel Beauchamp, R. ; Robert Upp, J. ; Rajaraman, Srinivasan ; Townsend, Courtney ; Thompson, James C. / Bombesin stimulates growth of human gastrinoma. In: Surgery. 1992 ; Vol. 112, No. 6. pp. 1059-1065.
@article{3a4c6beaef204e4781a540d6e838ec3b,
title = "Bombesin stimulates growth of human gastrinoma",
abstract = "Background. We have previously reported the first establishment and characterization of a functioning human gastrinoma (PT) xenograft. Bombesin, the equivalent of the mammalian gastrin-releasing peptide, has trophic effects on normal and neoplastic tissues of the gastrointestinal tract; the effects of gut hormones on the growth of gastrinoma are not known. The purpose of this study was twofold: (1) to determine the presence of various gut peptides in PT and (2) to determine the effect of bombesin on the growth of PT xenografts. Methods. PT tumors were examined for expression (mRNA and protein) of various gut peptides by Northern hybridization and immunohistochemistry. In addition, PT xenografts were implanted as 3 mm 2 pieces bilaterally subcutaneously in athymic nude mice. Mice were divided into two groups to receive either bombesin (5 μg/kg) or saline administered as intraperitoneal injections every 8 hours. Tumor area was measured twice weekly until mice were sacrificed (day 28), when tumor and normal pancreas were removed, weighed, and assayed for DNA and protein content. Results. Both mRNAs and peptides of gastrin and chromogranin A were present in PT tumors. Bombesin significantly stimulated growth of PT tumors from day 18 until mice were sacrificed (day 28). As expected, bombesin stimulated pancreatic growth. Conclusions. We have demonstrated for the first time that bombesin is a trophic hormone for gastrinoma. The unique cell line PT contains gastrin and chromogranin A and will be a useful model to define the biologic mechanisms controlling the growth of human gastrinomas.",
author = "Chung, {Dai H.} and {Mark Evers}, B. and {Daniel Beauchamp}, R. and {Robert Upp}, J. and Srinivasan Rajaraman and Courtney Townsend and Thompson, {James C.}",
year = "1992",
language = "English (US)",
volume = "112",
pages = "1059--1065",
journal = "Surgery",
issn = "0039-6060",
publisher = "Mosby Inc.",
number = "6",

}

TY - JOUR

T1 - Bombesin stimulates growth of human gastrinoma

AU - Chung, Dai H.

AU - Mark Evers, B.

AU - Daniel Beauchamp, R.

AU - Robert Upp, J.

AU - Rajaraman, Srinivasan

AU - Townsend, Courtney

AU - Thompson, James C.

PY - 1992

Y1 - 1992

N2 - Background. We have previously reported the first establishment and characterization of a functioning human gastrinoma (PT) xenograft. Bombesin, the equivalent of the mammalian gastrin-releasing peptide, has trophic effects on normal and neoplastic tissues of the gastrointestinal tract; the effects of gut hormones on the growth of gastrinoma are not known. The purpose of this study was twofold: (1) to determine the presence of various gut peptides in PT and (2) to determine the effect of bombesin on the growth of PT xenografts. Methods. PT tumors were examined for expression (mRNA and protein) of various gut peptides by Northern hybridization and immunohistochemistry. In addition, PT xenografts were implanted as 3 mm 2 pieces bilaterally subcutaneously in athymic nude mice. Mice were divided into two groups to receive either bombesin (5 μg/kg) or saline administered as intraperitoneal injections every 8 hours. Tumor area was measured twice weekly until mice were sacrificed (day 28), when tumor and normal pancreas were removed, weighed, and assayed for DNA and protein content. Results. Both mRNAs and peptides of gastrin and chromogranin A were present in PT tumors. Bombesin significantly stimulated growth of PT tumors from day 18 until mice were sacrificed (day 28). As expected, bombesin stimulated pancreatic growth. Conclusions. We have demonstrated for the first time that bombesin is a trophic hormone for gastrinoma. The unique cell line PT contains gastrin and chromogranin A and will be a useful model to define the biologic mechanisms controlling the growth of human gastrinomas.

AB - Background. We have previously reported the first establishment and characterization of a functioning human gastrinoma (PT) xenograft. Bombesin, the equivalent of the mammalian gastrin-releasing peptide, has trophic effects on normal and neoplastic tissues of the gastrointestinal tract; the effects of gut hormones on the growth of gastrinoma are not known. The purpose of this study was twofold: (1) to determine the presence of various gut peptides in PT and (2) to determine the effect of bombesin on the growth of PT xenografts. Methods. PT tumors were examined for expression (mRNA and protein) of various gut peptides by Northern hybridization and immunohistochemistry. In addition, PT xenografts were implanted as 3 mm 2 pieces bilaterally subcutaneously in athymic nude mice. Mice were divided into two groups to receive either bombesin (5 μg/kg) or saline administered as intraperitoneal injections every 8 hours. Tumor area was measured twice weekly until mice were sacrificed (day 28), when tumor and normal pancreas were removed, weighed, and assayed for DNA and protein content. Results. Both mRNAs and peptides of gastrin and chromogranin A were present in PT tumors. Bombesin significantly stimulated growth of PT tumors from day 18 until mice were sacrificed (day 28). As expected, bombesin stimulated pancreatic growth. Conclusions. We have demonstrated for the first time that bombesin is a trophic hormone for gastrinoma. The unique cell line PT contains gastrin and chromogranin A and will be a useful model to define the biologic mechanisms controlling the growth of human gastrinomas.

UR - http://www.scopus.com/inward/record.url?scp=0027066767&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027066767&partnerID=8YFLogxK

M3 - Article

VL - 112

SP - 1059

EP - 1065

JO - Surgery

JF - Surgery

SN - 0039-6060

IS - 6

ER -