Bombesin stimulates in vitro growth of human breast cancer independent of estrogen receptors status

Richard J. Bold, Jin Ishizuka, Chong Z. Yao, Courtney Townsend, James C. Thompson

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Background: Approximately 180,000 women will be found to have breast cancer this year in the United States. Chemotherapy has limited success in advanced disease and the effect of tamoxifen appears to require a functional estrogen-receptor (ER). Our aim was to determine whether bombesin (BBS) regulates growth of human breast cancer cells. Methods: Estrogen-dependent (MCF-7), estrogen-responsive (ZR-75-1) and estrogen-independent (MDA-MB-231) human breast cancer cells were studied. Receptors were identified by cross-linking methods and radioligand binding assays; intracellular calcium ([Ca2+](i)) was measured after BBS treatment to confirm functional status of the receptor; and the effect of BBS on cell growth was measured directly. Results: All three cell lines had a high affinity BBS receptor (K(d) = 1-7 nM; molecular weight 75 kDa). BBS stimulated [Ca2+]i levels as well as cell growth in all three cell lines; the trophic effect was blocked by BBS receptor antagonists. Conclusions: We conclude that BBS is trophic for human breast cancers independent of ER status, and that antagonism of the BBS receptor may be a useful target for hormonal therapy in ER-negative breast cancer.

Original languageEnglish (US)
Pages (from-to)4051-4056
Number of pages6
JournalAnticancer Research
Volume18
Issue number6 A
StatePublished - 1998

Fingerprint

Bombesin
Estrogen Receptors
Bombesin Receptors
Breast Neoplasms
Growth
Estrogens
Cell Line
Radioligand Assay
Tamoxifen
Molecular Weight
In Vitro Techniques
Calcium
Drug Therapy
Therapeutics

Keywords

  • Bombesin
  • Breast cancer
  • Estrogen
  • Receptor

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Bold, R. J., Ishizuka, J., Yao, C. Z., Townsend, C., & Thompson, J. C. (1998). Bombesin stimulates in vitro growth of human breast cancer independent of estrogen receptors status. Anticancer Research, 18(6 A), 4051-4056.

Bombesin stimulates in vitro growth of human breast cancer independent of estrogen receptors status. / Bold, Richard J.; Ishizuka, Jin; Yao, Chong Z.; Townsend, Courtney; Thompson, James C.

In: Anticancer Research, Vol. 18, No. 6 A, 1998, p. 4051-4056.

Research output: Contribution to journalArticle

Bold, RJ, Ishizuka, J, Yao, CZ, Townsend, C & Thompson, JC 1998, 'Bombesin stimulates in vitro growth of human breast cancer independent of estrogen receptors status', Anticancer Research, vol. 18, no. 6 A, pp. 4051-4056.
Bold, Richard J. ; Ishizuka, Jin ; Yao, Chong Z. ; Townsend, Courtney ; Thompson, James C. / Bombesin stimulates in vitro growth of human breast cancer independent of estrogen receptors status. In: Anticancer Research. 1998 ; Vol. 18, No. 6 A. pp. 4051-4056.
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