Bone toxicity of locally applied aminoglycosides.

T. Miclau, M. L. Edin, G. E. Lester, Ronald Lindsey, L. E. Dahners

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98 Scopus citations

Abstract

The effect of tobramycin on osteoblasts was studied. Osteoblast-like cells from the MG-63 human osteosarcoma cell line were grown in antibiotic-free media and exposed to concentrations of tobramycin: first at order of magnitude intervals between 0 and 10,000 micrograms/ml, and then at closer intervals between 100 and 1,000 micrograms/ml to more accurately define toxic levels. Cell number and 3H-thymidine incorporation at 0, 24 and 72 h were determined. Similarly, primary cultures of rat osteoblasts were exposed to the same concentrations of tobramycin to confirm the validity of the MG-63 cell line as a model for nontransformed cells. The results of this study demonstrate that local levels of tobramycin of < or = 200 micrograms/ml have little or no effect on osteoblast replication. Concentrations of 400 micrograms/ml significantly decreased cell replication, and concentrations of 10,000 micrograms/ml caused cell death. Results obtained with primary rat osteoblast cultures were similar to those obtained from the MG-63 cultures at the tested tobramycin concentrations.

Original languageEnglish (US)
Pages (from-to)401-406
Number of pages6
JournalJournal of Orthopaedic Trauma
Volume9
Issue number5
StatePublished - 1995
Externally publishedYes

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ASJC Scopus subject areas

  • Surgery
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Miclau, T., Edin, M. L., Lester, G. E., Lindsey, R., & Dahners, L. E. (1995). Bone toxicity of locally applied aminoglycosides. Journal of Orthopaedic Trauma, 9(5), 401-406.