TY - JOUR
T1 - Bornavirus closely associates and segregates with host chromosomes to ensure persistent intranuclear infection
AU - Matsumoto, Yusuke
AU - Hayashi, Yohei
AU - Omori, Hiroko
AU - Honda, Tomoyuki
AU - Daito, Takuji
AU - Horie, Masayuki
AU - Ikuta, Kazuyoshi
AU - Fujino, Kan
AU - Nakamura, Shoko
AU - Schneider, Urs
AU - Chase, Geoffrey
AU - Yoshimori, Tamotsu
AU - Schwemmle, Martin
AU - Tomonaga, Keizo
N1 - Funding Information:
We thank Dr. K. Saito of DNA-chip Development Center for Infectious Diseases (RIMD, Osaka University) for mass spectrometry analysis. This study was supported in part by Funding Program for Next Generation World-Leading Researchers (NEXT program) from Japan Society for the Promotion of Science (JSPS) (K.T.), Grants-in-aid for Scientific Research on Priority Areas (Infection and Host Responses; Matrix of Infection Phenomena) from Ministry of Education, Culture, Sports, Science and Technology (MEXT) (K.T.), and PRESTO from Japan Science and Technology Agency (JST) (K.T.) and the Deutsche Forschungsgemeinschaft (DFG-SCHW632-/10-2) (M.S.).
PY - 2012/5/17
Y1 - 2012/5/17
N2 - Bornaviruses are nonsegmented negative-strand RNA viruses that establish a persistent infection in the nucleus and occasionally integrate a DNA genome copy into the host chromosomal DNA. However, how these viruses achieve intranuclear infection remains unclear. We show that Borna disease virus (BDV), a mammalian bornavirus, closely associates with the cellular chromosome to ensure intranuclear infection. BDV generates viral factories within the nucleus using host chromatin as a scaffold. In addition, the viral ribonucleoprotein (RNP) interacts directly with the host chromosome throughout the cell cycle, using core histones as a docking platform. HMGB1, a host chromatin-remodeling DNA architectural protein, is required to stabilize RNP on chromosomes and for efficient BDV RNA transcription in the nucleus. During metaphase, the association of RNP with mitotic chromosomes allows the viral RNA to segregate into daughter cells and ensure persistent infection. Thus, bornaviruses likely evolved a chromosome-dependent life cycle to achieve stable intranuclear infection.
AB - Bornaviruses are nonsegmented negative-strand RNA viruses that establish a persistent infection in the nucleus and occasionally integrate a DNA genome copy into the host chromosomal DNA. However, how these viruses achieve intranuclear infection remains unclear. We show that Borna disease virus (BDV), a mammalian bornavirus, closely associates with the cellular chromosome to ensure intranuclear infection. BDV generates viral factories within the nucleus using host chromatin as a scaffold. In addition, the viral ribonucleoprotein (RNP) interacts directly with the host chromosome throughout the cell cycle, using core histones as a docking platform. HMGB1, a host chromatin-remodeling DNA architectural protein, is required to stabilize RNP on chromosomes and for efficient BDV RNA transcription in the nucleus. During metaphase, the association of RNP with mitotic chromosomes allows the viral RNA to segregate into daughter cells and ensure persistent infection. Thus, bornaviruses likely evolved a chromosome-dependent life cycle to achieve stable intranuclear infection.
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U2 - 10.1016/j.chom.2012.04.009
DO - 10.1016/j.chom.2012.04.009
M3 - Article
C2 - 22607802
AN - SCOPUS:84861166952
SN - 1931-3128
VL - 11
SP - 492
EP - 503
JO - Cell Host and Microbe
JF - Cell Host and Microbe
IS - 5
ER -