Bovine vaccinia outbreaks

Detection and isolation of vaccinia virus in milk samples

Jônatas S. Abrahão, Tércia M L Oliveira, Rafael Kroon Campos, Marieta C. Madureira, Erna G. Kroon, Zélia I P Lobato

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The vaccinia virus (VACV), which causes exanthemous lesions in dairy cattle and humans, has been associated with several bovine vaccinia outbreaks in Brazil. Currently, no data are available about the safety of milk produced in VACV-affected areas. In this study, 47 milk samples were collected during bovine vaccinia outbreaks and submitted to viral isolation, DNA detection, and nucleotide sequencing of the conserved tk gene. The appearance of characteristic white pocks on the chorioallantoic membranes of chicken eggs, in association with viral cytopathic effects in chicken embryo fibroblasts and phylogenetic data, strongly suggest milk contamination by VACV. This is the first report of VACV detection in and isolation from milk.

Original languageEnglish (US)
Pages (from-to)1141-1146
Number of pages6
JournalFoodborne Pathogens and Disease
Volume6
Issue number9
DOIs
StatePublished - Nov 1 2009
Externally publishedYes

Fingerprint

Vaccinia
Vaccinia virus
Vaccinium
Disease Outbreaks
Milk
milk
cattle
Chickens
Viral Cytopathogenic Effect
Chorioallantoic Membrane
chorioallantoic membrane
sampling
cytopathogenicity
chicken eggs
Viral DNA
lesions (animal)
Eggs
dairy cattle
fibroblasts
Brazil

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology
  • Food Science
  • Animal Science and Zoology

Cite this

Abrahão, J. S., Oliveira, T. M. L., Kroon Campos, R., Madureira, M. C., Kroon, E. G., & Lobato, Z. I. P. (2009). Bovine vaccinia outbreaks: Detection and isolation of vaccinia virus in milk samples. Foodborne Pathogens and Disease, 6(9), 1141-1146. https://doi.org/10.1089/fpd.2009.0324

Bovine vaccinia outbreaks : Detection and isolation of vaccinia virus in milk samples. / Abrahão, Jônatas S.; Oliveira, Tércia M L; Kroon Campos, Rafael; Madureira, Marieta C.; Kroon, Erna G.; Lobato, Zélia I P.

In: Foodborne Pathogens and Disease, Vol. 6, No. 9, 01.11.2009, p. 1141-1146.

Research output: Contribution to journalArticle

Abrahão, Jônatas S. ; Oliveira, Tércia M L ; Kroon Campos, Rafael ; Madureira, Marieta C. ; Kroon, Erna G. ; Lobato, Zélia I P. / Bovine vaccinia outbreaks : Detection and isolation of vaccinia virus in milk samples. In: Foodborne Pathogens and Disease. 2009 ; Vol. 6, No. 9. pp. 1141-1146.
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