Bovine vaccinia outbreaks: Detection and isolation of vaccinia virus in milk samples

Jônatas S. Abrahão; Tércia M.L. Oliveira; Rafael K. Campos; Marieta C. Madureira; Erna G. Kroon; Zélia I.P. Lobato

doi:10.1089/fpd.2009.0324

Bovine vaccinia outbreaks: Detection and isolation of vaccinia virus in milk samples

Jônatas S. Abrahão, Tércia M.L. Oliveira, Rafael K. Campos, Marieta C. Madureira, Erna G. Kroon, Zélia I.P. Lobato

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

The vaccinia virus (VACV), which causes exanthemous lesions in dairy cattle and humans, has been associated with several bovine vaccinia outbreaks in Brazil. Currently, no data are available about the safety of milk produced in VACV-affected areas. In this study, 47 milk samples were collected during bovine vaccinia outbreaks and submitted to viral isolation, DNA detection, and nucleotide sequencing of the conserved tk gene. The appearance of characteristic white pocks on the chorioallantoic membranes of chicken eggs, in association with viral cytopathic effects in chicken embryo fibroblasts and phylogenetic data, strongly suggest milk contamination by VACV. This is the first report of VACV detection in and isolation from milk.

Original language	English (US)
Pages (from-to)	1141-1146
Number of pages	6
Journal	Foodborne Pathogens and Disease
Volume	6
Issue number	9
DOIs	https://doi.org/10.1089/fpd.2009.0324
State	Published - Nov 1 2009
Externally published	Yes

ASJC Scopus subject areas

Applied Microbiology and Biotechnology
Food Science
Animal Science and Zoology
Microbiology

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10.1089/fpd.2009.0324

Cite this

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title = "Bovine vaccinia outbreaks: Detection and isolation of vaccinia virus in milk samples",

abstract = "The vaccinia virus (VACV), which causes exanthemous lesions in dairy cattle and humans, has been associated with several bovine vaccinia outbreaks in Brazil. Currently, no data are available about the safety of milk produced in VACV-affected areas. In this study, 47 milk samples were collected during bovine vaccinia outbreaks and submitted to viral isolation, DNA detection, and nucleotide sequencing of the conserved tk gene. The appearance of characteristic white pocks on the chorioallantoic membranes of chicken eggs, in association with viral cytopathic effects in chicken embryo fibroblasts and phylogenetic data, strongly suggest milk contamination by VACV. This is the first report of VACV detection in and isolation from milk.",

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T2 - Detection and isolation of vaccinia virus in milk samples

AU - Abrahão, Jônatas S.

AU - Oliveira, Tércia M.L.

AU - Campos, Rafael K.

AU - Madureira, Marieta C.

AU - Kroon, Erna G.

AU - Lobato, Zélia I.P.

PY - 2009/11/1

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AB - The vaccinia virus (VACV), which causes exanthemous lesions in dairy cattle and humans, has been associated with several bovine vaccinia outbreaks in Brazil. Currently, no data are available about the safety of milk produced in VACV-affected areas. In this study, 47 milk samples were collected during bovine vaccinia outbreaks and submitted to viral isolation, DNA detection, and nucleotide sequencing of the conserved tk gene. The appearance of characteristic white pocks on the chorioallantoic membranes of chicken eggs, in association with viral cytopathic effects in chicken embryo fibroblasts and phylogenetic data, strongly suggest milk contamination by VACV. This is the first report of VACV detection in and isolation from milk.

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Bovine vaccinia outbreaks: Detection and isolation of vaccinia virus in milk samples

Abstract

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