Bovine viral diarrhea virus type 1 - and type 2-specific bovine T lymphocyte - Subset responses following modified-live virus vaccination

Kristina Ripplinger, Mark Quade, Brett Terhaar, James Roth, Janice Endsley

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


Expression of CD25 (interleukin-2 receptor a chain) was used to monitor antigen-specific activation of T lymphocyte subsets (CD4+, CD8 +, and gd T cells) from cattle immunized with modified-live virus (MLV) bovine viral diarrhea virus (BVDV) vaccines. Two groups of 15 animals each were vaccinated with one dose of either BVDV genotype 1 (BVDV-1) or BVDV-1 and BVDV genotype 2 (BVDV-1/2). Six animals negative for both BVDV antibody and BVDV virus were used as negative controls. Three animals vaccinated 7 and 5 weeks before the start of the experiment with MLV BVDV-1 vaccine served as positive controls. Blood samples were taken from the negative control group, the positive control group, and the BVDV-1/2 group 0, 21, 35, 60, and 90 days after vaccination. Blood samples were taken from the BVDV-1 group 0, 21, and 90 days after vaccination. Isolated peripheral blood lymphocytes from immunized and control animals were incubated for 5 days with and without BVDV-1 or BVDV-2. Compared with nonvaccinated animals, a significant (P < .05) increase in expression of CD25 by CD4+ (60 days), CD8+, and gd T (35 to 90 days) lymphocytes from the group given BVDV-1/2 was detected following in vitro exposure to BVDV-1 or BVDV-2 after vaccination. The CD8+ and gd T cells from the group vaccinated with BVDV-1 had significantly (P < .05) increased expression of CD25 compared with nonvaccinates following postvaccination exposure to in vitro BVDV-1 but not to BVDV-2. There was no significant difference between the two vaccinated groups in CD25 expression on any of the T cell subsets in response to BVDV-1 or BVDV-2 exposure. A single administration of MLV BVDV vaccine may be more effective at stimulating CD8+ and gd T cell"specific immune responses to the homologous genotype than to the heterologous genotype.

Original languageEnglish (US)
JournalVeterinary Therapeutics
Issue number4
StatePublished - 2002
Externally publishedYes

ASJC Scopus subject areas

  • General Veterinary


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