Bradykinin induces superoxide anion release from human endothelial cells

James A. Holland, Kirkwood A. Pritchard, Miguel Pappolla, Michael S. Wolin, Nancy J. Rogers, Michael B. Stemerman

Research output: Contribution to journalArticle

122 Citations (Scopus)

Abstract

The time-dependent release of superoxide anion (O2 -) from bradykinin (Bk) -stimulated human umbilical vein endothelial cells (EC) was measured as the superoxide dismutase-inhibitable reduction of ferricytochrome C employing a novel application of microspectrophotometry. In the absence of Bk, O2 - release by EC was not detectable. EC exposure to Bk (10-6 to 10-5 M) resulted in a rapid release of O2 -. The release of O2 - occurred within 5 minutes of exposure. O2 - release was partially inhibited by indomethacin (63 ± 6%), thus suggesting that arachidonic acid metabolism, through cyclooxygenase, contributes to EC O2 - production. EC O2 - release may be an important component in the pathophysiologic actions of Bk on vascular function.

Original languageEnglish (US)
Pages (from-to)21-25
Number of pages5
JournalJournal of Cellular Physiology
Volume143
Issue number1
StatePublished - 1990
Externally publishedYes

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Endothelial cells
Bradykinin
Superoxides
Endothelial Cells
Microspectrophotometry
Human Umbilical Vein Endothelial Cells
Prostaglandin-Endoperoxide Synthases
Arachidonic Acid
Indomethacin
Superoxide Dismutase
Blood Vessels
Metabolism

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Holland, J. A., Pritchard, K. A., Pappolla, M., Wolin, M. S., Rogers, N. J., & Stemerman, M. B. (1990). Bradykinin induces superoxide anion release from human endothelial cells. Journal of Cellular Physiology, 143(1), 21-25.

Bradykinin induces superoxide anion release from human endothelial cells. / Holland, James A.; Pritchard, Kirkwood A.; Pappolla, Miguel; Wolin, Michael S.; Rogers, Nancy J.; Stemerman, Michael B.

In: Journal of Cellular Physiology, Vol. 143, No. 1, 1990, p. 21-25.

Research output: Contribution to journalArticle

Holland, JA, Pritchard, KA, Pappolla, M, Wolin, MS, Rogers, NJ & Stemerman, MB 1990, 'Bradykinin induces superoxide anion release from human endothelial cells', Journal of Cellular Physiology, vol. 143, no. 1, pp. 21-25.
Holland JA, Pritchard KA, Pappolla M, Wolin MS, Rogers NJ, Stemerman MB. Bradykinin induces superoxide anion release from human endothelial cells. Journal of Cellular Physiology. 1990;143(1):21-25.
Holland, James A. ; Pritchard, Kirkwood A. ; Pappolla, Miguel ; Wolin, Michael S. ; Rogers, Nancy J. ; Stemerman, Michael B. / Bradykinin induces superoxide anion release from human endothelial cells. In: Journal of Cellular Physiology. 1990 ; Vol. 143, No. 1. pp. 21-25.
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