Bradykinin induces superoxide anion release from human endothelial cells

James A. Holland, Kirkwood A. Pritchard, Miguel Pappolla, Michael S. Wolin, Nancy J. Rogers, Michael B. Stemerman

Research output: Contribution to journalArticle

123 Scopus citations


The time-dependent release of superoxide anion (O2 -) from bradykinin (Bk) -stimulated human umbilical vein endothelial cells (EC) was measured as the superoxide dismutase-inhibitable reduction of ferricytochrome C employing a novel application of microspectrophotometry. In the absence of Bk, O2 - release by EC was not detectable. EC exposure to Bk (10-6 to 10-5 M) resulted in a rapid release of O2 -. The release of O2 - occurred within 5 minutes of exposure. O2 - release was partially inhibited by indomethacin (63 ± 6%), thus suggesting that arachidonic acid metabolism, through cyclooxygenase, contributes to EC O2 - production. EC O2 - release may be an important component in the pathophysiologic actions of Bk on vascular function.

Original languageEnglish (US)
Pages (from-to)21-25
Number of pages5
JournalJournal of Cellular Physiology
Issue number1
StatePublished - 1990
Externally publishedYes


ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Holland, J. A., Pritchard, K. A., Pappolla, M., Wolin, M. S., Rogers, N. J., & Stemerman, M. B. (1990). Bradykinin induces superoxide anion release from human endothelial cells. Journal of Cellular Physiology, 143(1), 21-25.