Bradykinin induces superoxide anion release from human endothelial cells

James A. Holland, Kirkwood A. Pritchard, Miguel A. Pappolla, Michael S. Wolin, Nancy J. Rogers, Michael B. Stemerman

Research output: Contribution to journalArticlepeer-review

124 Scopus citations

Abstract

The time‐dependent release of superoxide anion (O 2−) from bradykinin (Bk)‐stimulated human umbilical vein endothelial cells (EC) was measured as the superoxide dismutase‐inhibitable reduction of ferricytochrome C employing a novel application of microspectrophotometry. In the absence of Bk, O 2− release by EC was not detectable. EC exposure to Bk (10−6 to 10−5 M) resulted in a rapid release of O 2−. The release of O 2− occurred within 5 minutes of exposure. O 2− release was partially inhibited by indomethacin (63 ± 6%), thus suggesting that arachidonic acid metabolism, through cyclooxygenase, contributes to EC O 2− production. EC O 2− release may be an important component in the pathophysiologic actions of Bk on vascular function.

Original languageEnglish (US)
Pages (from-to)21-25
Number of pages5
JournalJournal of Cellular Physiology
Volume143
Issue number1
DOIs
StatePublished - Apr 1990

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

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