The time-dependent release of superoxide anion (O2 -) from bradykinin (Bk) -stimulated human umbilical vein endothelial cells (EC) was measured as the superoxide dismutase-inhibitable reduction of ferricytochrome C employing a novel application of microspectrophotometry. In the absence of Bk, O2 - release by EC was not detectable. EC exposure to Bk (10-6 to 10-5 M) resulted in a rapid release of O2 -. The release of O2 - occurred within 5 minutes of exposure. O2 - release was partially inhibited by indomethacin (63 ± 6%), thus suggesting that arachidonic acid metabolism, through cyclooxygenase, contributes to EC O2 - production. EC O2 - release may be an important component in the pathophysiologic actions of Bk on vascular function.
|Original language||English (US)|
|Number of pages||5|
|Journal||Journal of Cellular Physiology|
|State||Published - 1990|
ASJC Scopus subject areas
- Clinical Biochemistry
- Cell Biology