Brain-derived neurotrophic factor (BDNF) protects cultured rat cerebellar granule neurons against glucose deprivation-induced apoptosis

L. Tong, R. Perez-Polo

    Research output: Contribution to journalArticle

    23 Citations (Scopus)

    Abstract

    In the present study, cell death induced by glucose deprivation in primary cultures of cerebellar granule neurons was examined. Glucose deprivation-induced apoptotic cell death was demonstrated using the terminal transferase-mediated (TdT) deoxyuridine triphosphate (d-UTP)-biotin nick end labeling (TUNEL) method and DNA fragmentation assays. When the effects of different neurotrophins on the survival of cerebellar granule neurons after glucose deprivation were assessed, BDNF, but not NT-3 or NGF, was found to protect cerebellar granule neurons against glucose deprivation-induced cell death. In addition, BDNF treatment increased c-Fos immunoreactivity in the cerebellar granule neurons. These results are consistent with the hypothesis that neuronal death due to glucose deprivation has a significant apoptotic component and that neurotrophins can protect against hypoglycemic damage.

    Original languageEnglish (US)
    Pages (from-to)905-914
    Number of pages10
    JournalJournal of Neural Transmission
    Volume105
    Issue number8-9
    DOIs
    StatePublished - 1998

    Fingerprint

    Brain-Derived Neurotrophic Factor
    Apoptosis
    Neurons
    Glucose
    Cell Death
    Nerve Growth Factors
    Nerve Growth Factor
    DNA Fragmentation
    Biotin
    Transferases
    Hypoglycemic Agents

    Keywords

    • Apoptosis
    • BDNF
    • Hypoglycemia
    • Neurotrophin

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    Brain-derived neurotrophic factor (BDNF) protects cultured rat cerebellar granule neurons against glucose deprivation-induced apoptosis. / Tong, L.; Perez-Polo, R.

    In: Journal of Neural Transmission, Vol. 105, No. 8-9, 1998, p. 905-914.

    Research output: Contribution to journalArticle

    @article{c7a4a60e975b4c6db93667853f4d4ffd,
    title = "Brain-derived neurotrophic factor (BDNF) protects cultured rat cerebellar granule neurons against glucose deprivation-induced apoptosis",
    abstract = "In the present study, cell death induced by glucose deprivation in primary cultures of cerebellar granule neurons was examined. Glucose deprivation-induced apoptotic cell death was demonstrated using the terminal transferase-mediated (TdT) deoxyuridine triphosphate (d-UTP)-biotin nick end labeling (TUNEL) method and DNA fragmentation assays. When the effects of different neurotrophins on the survival of cerebellar granule neurons after glucose deprivation were assessed, BDNF, but not NT-3 or NGF, was found to protect cerebellar granule neurons against glucose deprivation-induced cell death. In addition, BDNF treatment increased c-Fos immunoreactivity in the cerebellar granule neurons. These results are consistent with the hypothesis that neuronal death due to glucose deprivation has a significant apoptotic component and that neurotrophins can protect against hypoglycemic damage.",
    keywords = "Apoptosis, BDNF, Hypoglycemia, Neurotrophin",
    author = "L. Tong and R. Perez-Polo",
    year = "1998",
    doi = "10.1007/s007020050101",
    language = "English (US)",
    volume = "105",
    pages = "905--914",
    journal = "Journal of Neural Transmission",
    issn = "0300-9564",
    publisher = "Springer Verlag",
    number = "8-9",

    }

    TY - JOUR

    T1 - Brain-derived neurotrophic factor (BDNF) protects cultured rat cerebellar granule neurons against glucose deprivation-induced apoptosis

    AU - Tong, L.

    AU - Perez-Polo, R.

    PY - 1998

    Y1 - 1998

    N2 - In the present study, cell death induced by glucose deprivation in primary cultures of cerebellar granule neurons was examined. Glucose deprivation-induced apoptotic cell death was demonstrated using the terminal transferase-mediated (TdT) deoxyuridine triphosphate (d-UTP)-biotin nick end labeling (TUNEL) method and DNA fragmentation assays. When the effects of different neurotrophins on the survival of cerebellar granule neurons after glucose deprivation were assessed, BDNF, but not NT-3 or NGF, was found to protect cerebellar granule neurons against glucose deprivation-induced cell death. In addition, BDNF treatment increased c-Fos immunoreactivity in the cerebellar granule neurons. These results are consistent with the hypothesis that neuronal death due to glucose deprivation has a significant apoptotic component and that neurotrophins can protect against hypoglycemic damage.

    AB - In the present study, cell death induced by glucose deprivation in primary cultures of cerebellar granule neurons was examined. Glucose deprivation-induced apoptotic cell death was demonstrated using the terminal transferase-mediated (TdT) deoxyuridine triphosphate (d-UTP)-biotin nick end labeling (TUNEL) method and DNA fragmentation assays. When the effects of different neurotrophins on the survival of cerebellar granule neurons after glucose deprivation were assessed, BDNF, but not NT-3 or NGF, was found to protect cerebellar granule neurons against glucose deprivation-induced cell death. In addition, BDNF treatment increased c-Fos immunoreactivity in the cerebellar granule neurons. These results are consistent with the hypothesis that neuronal death due to glucose deprivation has a significant apoptotic component and that neurotrophins can protect against hypoglycemic damage.

    KW - Apoptosis

    KW - BDNF

    KW - Hypoglycemia

    KW - Neurotrophin

    UR - http://www.scopus.com/inward/record.url?scp=0031768724&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0031768724&partnerID=8YFLogxK

    U2 - 10.1007/s007020050101

    DO - 10.1007/s007020050101

    M3 - Article

    VL - 105

    SP - 905

    EP - 914

    JO - Journal of Neural Transmission

    JF - Journal of Neural Transmission

    SN - 0300-9564

    IS - 8-9

    ER -