Abstract
Mouse animal models are widely used as experimental tools in the research of
different diseases, including psychiatric, cognitive, neurodegenerative, and infectious diseases. In
this sense, brain from murine models is widely studied in the proteomics field, presenting
differences along the regions. However, even with that information, the diversity of functionality
of neurotransmitter receptors across brain regions remain elusive. Using electrophysiology of
mice synaptosomes from prefrontal cortex, hippocampus, striatum, olfactory bulb, cerebellum,
and medulla we aimed to investigate the differences between these regions in the signals of
GABA, AMPA and glycine receptors, which are major drivers of synaptic excitation (E) and
inhibition (I) in the nervous system. Electrophysiological E/I ratios were assessed by micro
transplantation of synaptic membranes (MSM). We found that GABA receptors peak amplitude
was significantly lower in hippocampus compared with olfactory bulb and striatum. The same
tendency that was observed for AMPA receptors, were the amplitude of AMPA receptors in
hippocampus was lower than in medulla and striatum samples. The E/I ratio was significantly
higher in mice medulla samples compared to olfactory bulb suggesting a distinct set point for
excitation to inhibition balance across brain regions. Understanding the differences in the
functional status of synaptic receptors and their relationship with the synapto-proteome in
different mice brain areas is a necessary step to translate those differences to human research in
different neuropathological conditions.
different diseases, including psychiatric, cognitive, neurodegenerative, and infectious diseases. In
this sense, brain from murine models is widely studied in the proteomics field, presenting
differences along the regions. However, even with that information, the diversity of functionality
of neurotransmitter receptors across brain regions remain elusive. Using electrophysiology of
mice synaptosomes from prefrontal cortex, hippocampus, striatum, olfactory bulb, cerebellum,
and medulla we aimed to investigate the differences between these regions in the signals of
GABA, AMPA and glycine receptors, which are major drivers of synaptic excitation (E) and
inhibition (I) in the nervous system. Electrophysiological E/I ratios were assessed by micro
transplantation of synaptic membranes (MSM). We found that GABA receptors peak amplitude
was significantly lower in hippocampus compared with olfactory bulb and striatum. The same
tendency that was observed for AMPA receptors, were the amplitude of AMPA receptors in
hippocampus was lower than in medulla and striatum samples. The E/I ratio was significantly
higher in mice medulla samples compared to olfactory bulb suggesting a distinct set point for
excitation to inhibition balance across brain regions. Understanding the differences in the
functional status of synaptic receptors and their relationship with the synapto-proteome in
different mice brain areas is a necessary step to translate those differences to human research in
different neuropathological conditions.
Original language | English (US) |
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State | Published - Oct 8 2024 |
Event | Society for Neuroscience 2024 - Chicago, Chicago, United States Duration: Oct 5 2024 → Oct 9 2024 https://www.sfn.org/meetings/neuroscience-2024/call-for-abstracts/neuroscience-2024-abstracts |
Conference
Conference | Society for Neuroscience 2024 |
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Abbreviated title | SfN 2024 |
Country/Territory | United States |
City | Chicago |
Period | 10/5/24 → 10/9/24 |
Internet address |