Braun lipoprotein (Lpp) contributes to virulence of yersiniae: Potential role of Lpp in inducing bubonic and pneumonic plague

Jian Sha, Stacy L. Agar, Wallace B. Baze, Juan Olano, Amin A. Fadl, Tatiana E. Erova, Shaofei Wang, Sheri M. Foltz, Giovanni Suarez, Vladimir Motin, Sadhana Chauhan, Gary R. Kumpel, Johnny Peterson, Ashok Chopra

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Yersinia pestis evolved from Y. pseudotuberculosis to become the causative agent of bubonic and pneumonic plague. We identified a homolog of the Salmonella enterica serovar Typhimurium lipoprotein (lpp) gene in Yersinia species and prepared lpp gene deletion mutants of Y. pseudotuberculosis YPIII, Y. pestis KIM/D27 (pigmentation locus minus), and Y. pestis CO92 with reduced virulence. Mice injected via the intraperitoneal route with 5 × 107 CFU of the μlpp KIM/D27 mutant survived a month, even though this would have constituted a lethal dose for the parental KIM/D27 strain. Subsequently, these Δlpp KIM/D27-injected mice were solidly protected against an intranasally administered, highly virulent Y. pestis CO92 strain when it was given as five 50% lethal doses (LD50). In a parallel study with the pneumonic plague mouse model, after 72 h postinfection, the lungs of animals infected with wild-type (WT) Y. pestis CO92 and given a subinhibitory dose of levofloxacin had acute inflammation, edema, and masses of bacteria, while the lung tissue appeared essentially normal in mice inoculated with the Δlpp mutant of CO92 and given the same dose of levofloxacin. Importantly, while WT Y. pestis CO92 could be detected in the bloodstreams and spleens of infected mice at 72 h postinfection, the Δlpp mutant of CO92 could not be detected in those organs. Furthermore, the levels of cytokines/chemokines detected in the sera were significantly lower in animals infected with the Δlpp mutant than in those infected with WT CO92. Additionally, the Δlpp mutant was more rapidly killed by macrophages than was the WT CO92 strain. These data provided evidence that the Δlpp mutants of yersiniae were significantly attenuated and could be useful tools in the development of new vaccines.

Original languageEnglish (US)
Pages (from-to)1390-1409
Number of pages20
JournalInfection and Immunity
Volume76
Issue number4
DOIs
StatePublished - Apr 2008

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Yersinia
Plague
Lipoproteins
Yersinia pestis
Virulence
Levofloxacin
Lethal Dose 50
Lung
Salmonella enterica
Gene Deletion
Pigmentation
Chemokines
Edema
Vaccines
Spleen
Macrophages
Cytokines
Inflammation
Bacteria

ASJC Scopus subject areas

  • Immunology

Cite this

Braun lipoprotein (Lpp) contributes to virulence of yersiniae : Potential role of Lpp in inducing bubonic and pneumonic plague. / Sha, Jian; Agar, Stacy L.; Baze, Wallace B.; Olano, Juan; Fadl, Amin A.; Erova, Tatiana E.; Wang, Shaofei; Foltz, Sheri M.; Suarez, Giovanni; Motin, Vladimir; Chauhan, Sadhana; Kumpel, Gary R.; Peterson, Johnny; Chopra, Ashok.

In: Infection and Immunity, Vol. 76, No. 4, 04.2008, p. 1390-1409.

Research output: Contribution to journalArticle

Sha, Jian ; Agar, Stacy L. ; Baze, Wallace B. ; Olano, Juan ; Fadl, Amin A. ; Erova, Tatiana E. ; Wang, Shaofei ; Foltz, Sheri M. ; Suarez, Giovanni ; Motin, Vladimir ; Chauhan, Sadhana ; Kumpel, Gary R. ; Peterson, Johnny ; Chopra, Ashok. / Braun lipoprotein (Lpp) contributes to virulence of yersiniae : Potential role of Lpp in inducing bubonic and pneumonic plague. In: Infection and Immunity. 2008 ; Vol. 76, No. 4. pp. 1390-1409.
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abstract = "Yersinia pestis evolved from Y. pseudotuberculosis to become the causative agent of bubonic and pneumonic plague. We identified a homolog of the Salmonella enterica serovar Typhimurium lipoprotein (lpp) gene in Yersinia species and prepared lpp gene deletion mutants of Y. pseudotuberculosis YPIII, Y. pestis KIM/D27 (pigmentation locus minus), and Y. pestis CO92 with reduced virulence. Mice injected via the intraperitoneal route with 5 × 107 CFU of the μlpp KIM/D27 mutant survived a month, even though this would have constituted a lethal dose for the parental KIM/D27 strain. Subsequently, these Δlpp KIM/D27-injected mice were solidly protected against an intranasally administered, highly virulent Y. pestis CO92 strain when it was given as five 50{\%} lethal doses (LD50). In a parallel study with the pneumonic plague mouse model, after 72 h postinfection, the lungs of animals infected with wild-type (WT) Y. pestis CO92 and given a subinhibitory dose of levofloxacin had acute inflammation, edema, and masses of bacteria, while the lung tissue appeared essentially normal in mice inoculated with the Δlpp mutant of CO92 and given the same dose of levofloxacin. Importantly, while WT Y. pestis CO92 could be detected in the bloodstreams and spleens of infected mice at 72 h postinfection, the Δlpp mutant of CO92 could not be detected in those organs. Furthermore, the levels of cytokines/chemokines detected in the sera were significantly lower in animals infected with the Δlpp mutant than in those infected with WT CO92. Additionally, the Δlpp mutant was more rapidly killed by macrophages than was the WT CO92 strain. These data provided evidence that the Δlpp mutants of yersiniae were significantly attenuated and could be useful tools in the development of new vaccines.",
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T2 - Potential role of Lpp in inducing bubonic and pneumonic plague

AU - Sha, Jian

AU - Agar, Stacy L.

AU - Baze, Wallace B.

AU - Olano, Juan

AU - Fadl, Amin A.

AU - Erova, Tatiana E.

AU - Wang, Shaofei

AU - Foltz, Sheri M.

AU - Suarez, Giovanni

AU - Motin, Vladimir

AU - Chauhan, Sadhana

AU - Kumpel, Gary R.

AU - Peterson, Johnny

AU - Chopra, Ashok

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AB - Yersinia pestis evolved from Y. pseudotuberculosis to become the causative agent of bubonic and pneumonic plague. We identified a homolog of the Salmonella enterica serovar Typhimurium lipoprotein (lpp) gene in Yersinia species and prepared lpp gene deletion mutants of Y. pseudotuberculosis YPIII, Y. pestis KIM/D27 (pigmentation locus minus), and Y. pestis CO92 with reduced virulence. Mice injected via the intraperitoneal route with 5 × 107 CFU of the μlpp KIM/D27 mutant survived a month, even though this would have constituted a lethal dose for the parental KIM/D27 strain. Subsequently, these Δlpp KIM/D27-injected mice were solidly protected against an intranasally administered, highly virulent Y. pestis CO92 strain when it was given as five 50% lethal doses (LD50). In a parallel study with the pneumonic plague mouse model, after 72 h postinfection, the lungs of animals infected with wild-type (WT) Y. pestis CO92 and given a subinhibitory dose of levofloxacin had acute inflammation, edema, and masses of bacteria, while the lung tissue appeared essentially normal in mice inoculated with the Δlpp mutant of CO92 and given the same dose of levofloxacin. Importantly, while WT Y. pestis CO92 could be detected in the bloodstreams and spleens of infected mice at 72 h postinfection, the Δlpp mutant of CO92 could not be detected in those organs. Furthermore, the levels of cytokines/chemokines detected in the sera were significantly lower in animals infected with the Δlpp mutant than in those infected with WT CO92. Additionally, the Δlpp mutant was more rapidly killed by macrophages than was the WT CO92 strain. These data provided evidence that the Δlpp mutants of yersiniae were significantly attenuated and could be useful tools in the development of new vaccines.

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