Breath markers of oxidative stress and airway inflammation in Seasonal Allergic Rhinitis

C. Gratziou, N. Rovina, Michael P. Makris, D. C.M. Simoes, Andreas Papapetropoulos, C. Roussos

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Oxidative stress (OS) is well documented in asthma, but so far little data has been reported in non-asthmatic patients with Seasonal Allergic Rhinitis (SAR). The aim of this study is to investigate the degree of OS and airway inflammation in patients with SAR, with and without concomitant Asthma (SAR +A), using breath markers in exhaled air and in Exhaled Breath Condensate (EBC). In addition, the effects of natural allergen exposure and intranasal steroid treatment on these markers were evaluated. Exhaled NO (eNO) and CO, combined with measurements of 8-Isoprostane (Iso-8), Leukotriene B4 (LTB4) and nitrate/nitrite in EBC, were performed in 23 patients, 11 with SAR and 12 with SAR+A, and 16 healthy subjects. Iso-8 and LTB4 were significantly increased in both groups of patients (median values 43.6 pg/ml and 138.4 pg/ml in SAR group; 38.9 pg/ml, and 164.6 pg/ml in SAR+A group respectively; p>0.05) compared to healthy subjects (18.6 pg/ml and 7.8 pg/ml; p<0.05). Nitrate/ nitrite and eNO levels were elevated in both groups compared to controls, but were significantly higher in the SAR+A compared to SAR group (nitrate/nitrite 9 μM and 3.9 μM; p=0.025; and eNO 18.5 ppb and 12.5 ppb, respectively; p>0.05). Nasal steroids caused significant reduction in LTB4 and 8-isoprostane levels in both groups of patients (p<0.05), while nitrate levels and eNO concentration were little affected by nasal treatment. OS markers were decreased at normal levels out of pollen season. Natural allergen exposure induces OS and airway inflammation, as assessed by measurements of markers in EBC and exhaled air, in patients with SAR who have no clinical signs of lower airway involvement. Besides, intranasal steroid treatment may have a regulatory role in the OS.

Original languageEnglish (US)
Pages (from-to)949-957
Number of pages9
JournalInternational Journal of Immunopathology and Pharmacology
Volume21
Issue number4
DOIs
StatePublished - Jan 1 2008
Externally publishedYes

Fingerprint

Seasonal Allergic Rhinitis
Oxidative Stress
Inflammation
8-epi-prostaglandin F2alpha
Leukotriene B4
Steroids
Nose
Nitrates
Allergens
Healthy Volunteers
Asthma
Air
Carbon Monoxide
Nitrites
Pollen
Therapeutics

Keywords

  • 8-isoprostane
  • Airway inflammation
  • Allergic rhinitis
  • Breath condensate
  • Exhaled NO
  • LTB
  • Nasal steroids
  • Nitrate/nitrite
  • Oxidative stress
  • Pollen season

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Pharmacology

Cite this

Breath markers of oxidative stress and airway inflammation in Seasonal Allergic Rhinitis. / Gratziou, C.; Rovina, N.; Makris, Michael P.; Simoes, D. C.M.; Papapetropoulos, Andreas; Roussos, C.

In: International Journal of Immunopathology and Pharmacology, Vol. 21, No. 4, 01.01.2008, p. 949-957.

Research output: Contribution to journalArticle

Gratziou, C. ; Rovina, N. ; Makris, Michael P. ; Simoes, D. C.M. ; Papapetropoulos, Andreas ; Roussos, C. / Breath markers of oxidative stress and airway inflammation in Seasonal Allergic Rhinitis. In: International Journal of Immunopathology and Pharmacology. 2008 ; Vol. 21, No. 4. pp. 949-957.
@article{96ad95f27e1844909d0cca83167ebe97,
title = "Breath markers of oxidative stress and airway inflammation in Seasonal Allergic Rhinitis",
abstract = "Oxidative stress (OS) is well documented in asthma, but so far little data has been reported in non-asthmatic patients with Seasonal Allergic Rhinitis (SAR). The aim of this study is to investigate the degree of OS and airway inflammation in patients with SAR, with and without concomitant Asthma (SAR +A), using breath markers in exhaled air and in Exhaled Breath Condensate (EBC). In addition, the effects of natural allergen exposure and intranasal steroid treatment on these markers were evaluated. Exhaled NO (eNO) and CO, combined with measurements of 8-Isoprostane (Iso-8), Leukotriene B4 (LTB4) and nitrate/nitrite in EBC, were performed in 23 patients, 11 with SAR and 12 with SAR+A, and 16 healthy subjects. Iso-8 and LTB4 were significantly increased in both groups of patients (median values 43.6 pg/ml and 138.4 pg/ml in SAR group; 38.9 pg/ml, and 164.6 pg/ml in SAR+A group respectively; p>0.05) compared to healthy subjects (18.6 pg/ml and 7.8 pg/ml; p<0.05). Nitrate/ nitrite and eNO levels were elevated in both groups compared to controls, but were significantly higher in the SAR+A compared to SAR group (nitrate/nitrite 9 μM and 3.9 μM; p=0.025; and eNO 18.5 ppb and 12.5 ppb, respectively; p>0.05). Nasal steroids caused significant reduction in LTB4 and 8-isoprostane levels in both groups of patients (p<0.05), while nitrate levels and eNO concentration were little affected by nasal treatment. OS markers were decreased at normal levels out of pollen season. Natural allergen exposure induces OS and airway inflammation, as assessed by measurements of markers in EBC and exhaled air, in patients with SAR who have no clinical signs of lower airway involvement. Besides, intranasal steroid treatment may have a regulatory role in the OS.",
keywords = "8-isoprostane, Airway inflammation, Allergic rhinitis, Breath condensate, Exhaled NO, LTB, Nasal steroids, Nitrate/nitrite, Oxidative stress, Pollen season",
author = "C. Gratziou and N. Rovina and Makris, {Michael P.} and Simoes, {D. C.M.} and Andreas Papapetropoulos and C. Roussos",
year = "2008",
month = "1",
day = "1",
doi = "10.1177/039463200802100419",
language = "English (US)",
volume = "21",
pages = "949--957",
journal = "International Journal of Immunopathology and Pharmacology",
issn = "0394-6320",
publisher = "Biomedical Research Press s.a.s.",
number = "4",

}

TY - JOUR

T1 - Breath markers of oxidative stress and airway inflammation in Seasonal Allergic Rhinitis

AU - Gratziou, C.

AU - Rovina, N.

AU - Makris, Michael P.

AU - Simoes, D. C.M.

AU - Papapetropoulos, Andreas

AU - Roussos, C.

PY - 2008/1/1

Y1 - 2008/1/1

N2 - Oxidative stress (OS) is well documented in asthma, but so far little data has been reported in non-asthmatic patients with Seasonal Allergic Rhinitis (SAR). The aim of this study is to investigate the degree of OS and airway inflammation in patients with SAR, with and without concomitant Asthma (SAR +A), using breath markers in exhaled air and in Exhaled Breath Condensate (EBC). In addition, the effects of natural allergen exposure and intranasal steroid treatment on these markers were evaluated. Exhaled NO (eNO) and CO, combined with measurements of 8-Isoprostane (Iso-8), Leukotriene B4 (LTB4) and nitrate/nitrite in EBC, were performed in 23 patients, 11 with SAR and 12 with SAR+A, and 16 healthy subjects. Iso-8 and LTB4 were significantly increased in both groups of patients (median values 43.6 pg/ml and 138.4 pg/ml in SAR group; 38.9 pg/ml, and 164.6 pg/ml in SAR+A group respectively; p>0.05) compared to healthy subjects (18.6 pg/ml and 7.8 pg/ml; p<0.05). Nitrate/ nitrite and eNO levels were elevated in both groups compared to controls, but were significantly higher in the SAR+A compared to SAR group (nitrate/nitrite 9 μM and 3.9 μM; p=0.025; and eNO 18.5 ppb and 12.5 ppb, respectively; p>0.05). Nasal steroids caused significant reduction in LTB4 and 8-isoprostane levels in both groups of patients (p<0.05), while nitrate levels and eNO concentration were little affected by nasal treatment. OS markers were decreased at normal levels out of pollen season. Natural allergen exposure induces OS and airway inflammation, as assessed by measurements of markers in EBC and exhaled air, in patients with SAR who have no clinical signs of lower airway involvement. Besides, intranasal steroid treatment may have a regulatory role in the OS.

AB - Oxidative stress (OS) is well documented in asthma, but so far little data has been reported in non-asthmatic patients with Seasonal Allergic Rhinitis (SAR). The aim of this study is to investigate the degree of OS and airway inflammation in patients with SAR, with and without concomitant Asthma (SAR +A), using breath markers in exhaled air and in Exhaled Breath Condensate (EBC). In addition, the effects of natural allergen exposure and intranasal steroid treatment on these markers were evaluated. Exhaled NO (eNO) and CO, combined with measurements of 8-Isoprostane (Iso-8), Leukotriene B4 (LTB4) and nitrate/nitrite in EBC, were performed in 23 patients, 11 with SAR and 12 with SAR+A, and 16 healthy subjects. Iso-8 and LTB4 were significantly increased in both groups of patients (median values 43.6 pg/ml and 138.4 pg/ml in SAR group; 38.9 pg/ml, and 164.6 pg/ml in SAR+A group respectively; p>0.05) compared to healthy subjects (18.6 pg/ml and 7.8 pg/ml; p<0.05). Nitrate/ nitrite and eNO levels were elevated in both groups compared to controls, but were significantly higher in the SAR+A compared to SAR group (nitrate/nitrite 9 μM and 3.9 μM; p=0.025; and eNO 18.5 ppb and 12.5 ppb, respectively; p>0.05). Nasal steroids caused significant reduction in LTB4 and 8-isoprostane levels in both groups of patients (p<0.05), while nitrate levels and eNO concentration were little affected by nasal treatment. OS markers were decreased at normal levels out of pollen season. Natural allergen exposure induces OS and airway inflammation, as assessed by measurements of markers in EBC and exhaled air, in patients with SAR who have no clinical signs of lower airway involvement. Besides, intranasal steroid treatment may have a regulatory role in the OS.

KW - 8-isoprostane

KW - Airway inflammation

KW - Allergic rhinitis

KW - Breath condensate

KW - Exhaled NO

KW - LTB

KW - Nasal steroids

KW - Nitrate/nitrite

KW - Oxidative stress

KW - Pollen season

UR - http://www.scopus.com/inward/record.url?scp=59849105047&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=59849105047&partnerID=8YFLogxK

U2 - 10.1177/039463200802100419

DO - 10.1177/039463200802100419

M3 - Article

C2 - 19144280

AN - SCOPUS:59849105047

VL - 21

SP - 949

EP - 957

JO - International Journal of Immunopathology and Pharmacology

JF - International Journal of Immunopathology and Pharmacology

SN - 0394-6320

IS - 4

ER -