Brefeldin A Block of Integrin-dependent Mechanosensitive ATP Release from Xenopus Oocytes Reveals a Novel Mechanism of Mechanotransduction

Rosario Maroto, Owen Hamill

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

Many animal cells release ATP into the extracellular medium, and often this release is mechanosensitive. However, the mechanisms underlying this release are not well understood. Using the luciferin-luciferase bioluminescent assay we demonstrate that a Xenopus oocyte releases ATP at a basal rate ∼ 0.01 fmol/s, and gentle mechanical stimulation can increase this to 50 fmol/s. Brefeldin A, nocodazole, and progesterone-induced-maturation block basal and mechanosensitive ATP release. These treatments share the common feature of disrupting the Golgi complex and vesicle trafficking to the cell surface and thereby block protein secretion and membrane protein insertion. We propose that ATP release occurs when protein transport vesicles enriched in ATP fuse with the plasma membrane. Collagenase, integrin-binding peptides, and cytochalasin D also block ATP release, indicating that extracellular, membrane and cytoskeletal elements are involved in the release process. Elevation of intracellular Ca2+ does not evoke ATP release but potentiates mechanosensitive ATP release. Our study indicates a novel mechanism of mechanotransduction that would allow cells to regulate membrane trafficking and protein transport/secretion in response to mechanical loading.

Original languageEnglish
Pages (from-to)23867-23872
Number of pages6
JournalJournal of Biological Chemistry
Volume276
Issue number26
DOIs
StatePublished - Jun 29 2001

Fingerprint

Brefeldin A
Xenopus
Integrins
Oocytes
Adenosine Triphosphate
Luminescent Measurements
Membranes
Nocodazole
Cytochalasin D
Transport Vesicles
Proteins
Membrane Transport Proteins
Golgi Apparatus
Collagenases
Electric fuses
Protein Transport
Cell membranes
Luciferases
Progesterone
Assays

ASJC Scopus subject areas

  • Biochemistry

Cite this

Brefeldin A Block of Integrin-dependent Mechanosensitive ATP Release from Xenopus Oocytes Reveals a Novel Mechanism of Mechanotransduction. / Maroto, Rosario; Hamill, Owen.

In: Journal of Biological Chemistry, Vol. 276, No. 26, 29.06.2001, p. 23867-23872.

Research output: Contribution to journalArticle

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