Broad and potently neutralizing monoclonal antibodies isolated from human survivors of New World hantavirus infection

Taylor B. Engdahl, Natalia A. Kuzmina, Adam Ronk, Chad Mire, Matthew A. Hyde, Nurgun Kose, Matthew D. Josleyn, Rachel E. Sutton, Apoorva Mehta, Rachael M. Wolters, Nicole M. Lloyd, Francisca R. Valdivieso, Thomas G. Ksiazek, Jay W. Hooper, Alexander Bukreyev, James E. Crowe

Research output: Contribution to journalArticlepeer-review

Abstract

New World hantaviruses (NWHs) are endemic in North and South America and cause hantavirus cardiopulmonary syndrome (HCPS), with a case fatality rate of up to 40%. Knowledge of the natural humoral immune response to NWH infection is limited. Here, we describe human monoclonal antibodies (mAbs) isolated from individuals previously infected with Sin Nombre virus (SNV) or Andes virus (ANDV). Most SNV-reactive antibodies show broad recognition and cross-neutralization of both New and Old World hantaviruses, while many ANDV-reactive antibodies show activity for ANDV only. mAbs ANDV-44 and SNV-53 compete for binding to a distinct site on the ANDV surface glycoprotein and show potently neutralizing activity to New and Old World hantaviruses. Four mAbs show therapeutic efficacy at clinically relevant doses in hamsters. These studies reveal a convergent and potently neutralizing human antibody response to NWHs and suggest therapeutic potential for human mAbs against HCPS.

Original languageEnglish (US)
Article number109086
JournalCell Reports
Volume35
Issue number5
DOIs
StatePublished - May 4 2021

Keywords

  • Andes virus
  • antibody
  • Bunyavirus
  • cross-reactivity
  • hantavirus
  • infection
  • Sin Nombre virus

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Broad and potently neutralizing monoclonal antibodies isolated from human survivors of New World hantavirus infection'. Together they form a unique fingerprint.

Cite this