Broad antiviral activity in tissues of crustaceans

Jingzhi Pan, Alexander Kurosky, Bo Xu, Ashok Chopra, Dorian H. Coppenhaver, Indra P. Singh, Samuel Baron

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Innate antiviral substances occur in vertebrates and may function as host defenses. Virus infections are common among invertebrates, but little is known about the ability of invertebrates to control viral infections. Pre-existing antiviral substances may be particularly important, since invertebrates lack the antiviral defense conferred by specific immunity. In our study, we found that tissue extracts of blue crab (Callinectes sapidus), shrimp (Penaeus setiferus), and crayfish (Procambarus clarkii) contained antiviral activities that inhibit a variety of DNA and RNA viruses, i.e. Sindbis virus (SB), vaccinia virus (VAC), vesicular stomatitis virus (VS), mengo virus (MENGO), banzi virus (BANZI) and poliomyelitis (POLIO). The concentration of inhibitory activity was relatively high, ranging from 102 to 216 U/g tissue for Sindbis virus, using the various tissue extracts. The other viruses were somewhat less sensitive to the inhibitor. The main antiviral activity in the inhibitor preparation from blue crab resided in an approximately 440 kDa fraction. It was inactivated significantly by lipid extraction, but not by proteinase K or glycosidases. The antiviral mechanism of the inhibitor from the blue crab was inhibition of virus attachment to eukaryotic cells, as evidenced by inhibitory activity at 4°C. These studies are among the first to show the existence of broadly active antiviral activities in aquatic crustaceans. These antiviral substances may function as innate host defenses in these species that lack specific antibody immunity and, therefore, merit further study. (C) 2000 Published by Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)39-47
Number of pages9
JournalAntiviral Research
Volume48
Issue number1
DOIs
StatePublished - 2000

Fingerprint

Antiviral Agents
Brachyura
Invertebrates
Sindbis Virus
Astacoidea
Tissue Extracts
Virus Diseases
Viruses
Immunity
Mengovirus
Penaeidae
Virus Attachment
Endopeptidase K
Vesicular Stomatitis
DNA Viruses
Vaccinia virus
Glycoside Hydrolases
RNA Viruses
Eukaryotic Cells
Poliomyelitis

Keywords

  • Antiviral
  • Broad spectrum
  • Crustaceans
  • Innate immunity

ASJC Scopus subject areas

  • Virology
  • Pharmacology

Cite this

Pan, J., Kurosky, A., Xu, B., Chopra, A., Coppenhaver, D. H., Singh, I. P., & Baron, S. (2000). Broad antiviral activity in tissues of crustaceans. Antiviral Research, 48(1), 39-47. https://doi.org/10.1016/S0166-3542(00)00117-0

Broad antiviral activity in tissues of crustaceans. / Pan, Jingzhi; Kurosky, Alexander; Xu, Bo; Chopra, Ashok; Coppenhaver, Dorian H.; Singh, Indra P.; Baron, Samuel.

In: Antiviral Research, Vol. 48, No. 1, 2000, p. 39-47.

Research output: Contribution to journalArticle

Pan, J, Kurosky, A, Xu, B, Chopra, A, Coppenhaver, DH, Singh, IP & Baron, S 2000, 'Broad antiviral activity in tissues of crustaceans', Antiviral Research, vol. 48, no. 1, pp. 39-47. https://doi.org/10.1016/S0166-3542(00)00117-0
Pan, Jingzhi ; Kurosky, Alexander ; Xu, Bo ; Chopra, Ashok ; Coppenhaver, Dorian H. ; Singh, Indra P. ; Baron, Samuel. / Broad antiviral activity in tissues of crustaceans. In: Antiviral Research. 2000 ; Vol. 48, No. 1. pp. 39-47.
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