Burn and smoke inhalation injury in sheep depletes vitamin E

Kinetic studies using deuterated tocopherols

M. G. Traber, K. Shimoda, K. Murakami, S. W. Leonard, Perenlei Enkhbaatar, L. D. Traber, D. L. Traber

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

To test the hypothesis that burn and smoke injury will deplete tissue α-tocopherol and cause its faster plasma disappearance, deuterium-labeled vitamin E was administered to sheep exposed to both surface skin burn and smoke insufflation, which cause injuries similar to those of human victims of fire accidents. Two different protocols were used: (1) deuterated vitamin E was administered orally with food at time 0 (just before injury) or (2) the labeled vitamin E was administered orally with food the day before injury. The animals, which had been operatively prepared seven days before, were anesthetized and then received both 40% body surface area third-degree burn and 48 breaths of cotton smoke or sham injuries. All were resuscitated with Ringer's lactate solution (4 ml/kg/% BSA burn/24 h) and mechanically ventilated. Blood samples were collected at various times after vitamin E dosing. In both studies the depletion of plasma α-tocopherol was faster in the injured sheep. The sheep given deuterated vitamin E 24 h before injury had similar maximum α-tocopherol concentrations at similar times. The exponential rates of α-tocopherol disappearance were 1.5 times greater and half-lives were 12 h shorter (p < 0.05) in the injured sheep. In separate studies, various tissues were obtained from sheep that were sacrificed from 4 to 48 h after injury. The liver α-tocopherol concentrations in sheep killed at various times after injury seem to show a linear decrease at a rate of 0.1 nmol α-tocopherol/g liver per hour, suggesting that the liver is supplying α-tocopherol to maintain the plasma and lung α-tocopherol concentrations, but that this injury is so severe the liver is unable to maintain lung α-tocopherol concentrations. These findings suggest that α-tocopherol should be administered to burn patients to prevent vitamin E depletion and to protect against oxidative stress from burn injury.

Original languageEnglish (US)
Pages (from-to)1421-1429
Number of pages9
JournalFree Radical Biology and Medicine
Volume42
Issue number9
DOIs
StatePublished - May 1 2007

Fingerprint

Smoke Inhalation Injury
Tocopherols
Vitamin E
Smoke
Sheep
Kinetics
Wounds and Injuries
Liver
Plasmas
Tissue
Food
Lung
Insufflation
Oxidative stress
Deuterium
Body Surface Area
Burns
Cotton
Accidents
Skin

Keywords

  • α-Tocopherol
  • Free radicals
  • Inflammation
  • Liver
  • Lung
  • Ovine model
  • Pharmacokinetics
  • Shock

ASJC Scopus subject areas

  • Medicine(all)
  • Toxicology
  • Clinical Biochemistry

Cite this

Burn and smoke inhalation injury in sheep depletes vitamin E : Kinetic studies using deuterated tocopherols. / Traber, M. G.; Shimoda, K.; Murakami, K.; Leonard, S. W.; Enkhbaatar, Perenlei; Traber, L. D.; Traber, D. L.

In: Free Radical Biology and Medicine, Vol. 42, No. 9, 01.05.2007, p. 1421-1429.

Research output: Contribution to journalArticle

Traber, M. G. ; Shimoda, K. ; Murakami, K. ; Leonard, S. W. ; Enkhbaatar, Perenlei ; Traber, L. D. ; Traber, D. L. / Burn and smoke inhalation injury in sheep depletes vitamin E : Kinetic studies using deuterated tocopherols. In: Free Radical Biology and Medicine. 2007 ; Vol. 42, No. 9. pp. 1421-1429.
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