Burn and smoke injury activates poly(adp-ribose)polymerase in circulating leukocytes

Eva Bartha, Sven Asmussen, Gabor Olah, Sebastian W. Rehberg, Yusuke Yamamoto, Daniel L. Traber, Csaba Szabo

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The nuclear enzyme poly(ADP-ribose)polymerase (PARP) plays a significant role in the pathogenesis of various forms of critical illness. DNA strand breaks induced by oxidative and nitrative stress trigger the activation of PARP, and PARP, in turn, mediates cell death and promotes proinflammatory responses. Until recently, most studies focused on the role of PARP in solid organs such as heart, liver, and kidney. We investigated the effect of burn and smoke inhalation on the levels of poly(ADP-ribosylated) proteins in circulating sheep leukocytes ex vivo. Adult female merino sheep were subjected to burn injury (2× 20% each flank, 3 degrees) and smoke inhalation injury (insufflated with a total of 48 breaths of cotton smoke) under deep anesthesia. Arterial and venous blood was collected at baseline, immediately after the injury and 1 to 24 h after the injury. Leukocytes were isolated with the Histopaque method. The levels of poly(ADP-ribosyl)ated proteins were determined by Western blotting. The amount of reactive oxygen species was quantified by the OxyBlot method. To examine whether PARP activation continues to increase ex vivo in the leukocytes, blood samples were incubated at room temperature or at 37°C for 3 h with or without the PARP inhibitor PJ34. To investigate whether the plasma of burn/smoke animals may trigger PARP activation, burn/smoke plasma was incubated with control leukocytes in vitro. The results show that burn and smoke injury induced a marked PARP activation in circulating leukocytes. The activity was the highest immediately after injury and at 1 h and decreased gradually over time. Incubation of whole blood at 37°C for 3 h significantly increased poly(ADP-ribose) levels, indicative of the presence of an ongoing cell activation process. In conclusion, PARP activity is elevated in leukocytes after burn and smoke inhalation injury, and the response parallels the time course of reactive oxygen species generation in these cells.

Original languageEnglish (US)
Pages (from-to)144-148
Number of pages5
JournalShock
Volume36
Issue number2
DOIs
StatePublished - Aug 2011

Fingerprint

Ribose
Poly(ADP-ribose) Polymerases
Smoke
Leukocytes
Wounds and Injuries
Smoke Inhalation Injury
Burns
Reactive Oxygen Species
Sheep
Inhalation Burns
Poly Adenosine Diphosphate Ribose
DNA Breaks
Critical Illness
Oxidative Stress
Cell Death
Anesthesia
Western Blotting
Kidney
Temperature
Liver

Keywords

  • Burn injury
  • leukocytes
  • poly(ADP-ribose)polymerase
  • smoke injury

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Emergency Medicine

Cite this

Bartha, E., Asmussen, S., Olah, G., Rehberg, S. W., Yamamoto, Y., Traber, D. L., & Szabo, C. (2011). Burn and smoke injury activates poly(adp-ribose)polymerase in circulating leukocytes. Shock, 36(2), 144-148. https://doi.org/10.1097/SHK.0b013e318212988c

Burn and smoke injury activates poly(adp-ribose)polymerase in circulating leukocytes. / Bartha, Eva; Asmussen, Sven; Olah, Gabor; Rehberg, Sebastian W.; Yamamoto, Yusuke; Traber, Daniel L.; Szabo, Csaba.

In: Shock, Vol. 36, No. 2, 08.2011, p. 144-148.

Research output: Contribution to journalArticle

Bartha, E, Asmussen, S, Olah, G, Rehberg, SW, Yamamoto, Y, Traber, DL & Szabo, C 2011, 'Burn and smoke injury activates poly(adp-ribose)polymerase in circulating leukocytes', Shock, vol. 36, no. 2, pp. 144-148. https://doi.org/10.1097/SHK.0b013e318212988c
Bartha E, Asmussen S, Olah G, Rehberg SW, Yamamoto Y, Traber DL et al. Burn and smoke injury activates poly(adp-ribose)polymerase in circulating leukocytes. Shock. 2011 Aug;36(2):144-148. https://doi.org/10.1097/SHK.0b013e318212988c
Bartha, Eva ; Asmussen, Sven ; Olah, Gabor ; Rehberg, Sebastian W. ; Yamamoto, Yusuke ; Traber, Daniel L. ; Szabo, Csaba. / Burn and smoke injury activates poly(adp-ribose)polymerase in circulating leukocytes. In: Shock. 2011 ; Vol. 36, No. 2. pp. 144-148.
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