c-myc gene product in rat azo-dye-induced hepatocarcinogenesis

T. Suzuki, K. Yamaguchi, K. Abe, C. Yanaihara, G. H. Greeley, T. Mochizuki, K. Iguchi, M. Hoshino, N. Yanaihara

    Research output: Contribution to journalArticle

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    Abstract

    Changes of c-myc gene-product levels were examined in liver extracts of rats having 3'-methyl-4-diaminoazobenzene-induced rat hepatocarcinogenesis, using an antibodies directed against a synthetic pentadecapeptide corresponding to the C-terminal 423-437 sequence of the predicted c-myc oncogene protein. After a 4 weeks treatment with 3'-methyl-4-diamino azobenzene (3'-Me-DAB), c-myc oncogene product-like immunoreactivity (c-myc-P-IR) was significantly increased as demonstrated by radioimmunoassay of rat liver extracts. In addition, immunohistochemical results of surviving and regenerating hepatocytes of 3 weeks and thereafter of 3-DAB-treated rats supported observations obtained by the radioimmunoassay. Hepatocytes of hyperplastic foci or neoplastic nodules were immunostained at about 80% in frequency. Foci of atypical hepatocellular proliferation less than 1 mm in diameter, which appeared from the 21st week after the start of treatment, harbored no or if present, a few immunoreactive cells, at 2.6% in frequency. Larger foci of atypical hepatocytes including cancer nodules had few or no immunoreactive cells. The present immunochemical approach which makes use of a synthetic peptide related to a functional protein (i.e. c-myc protein) is a worthwhile technique for detection of a wide range of functional proteins.

    Original languageEnglish (US)
    Pages (from-to)577-586
    Number of pages10
    JournalBiomedical Research
    Volume10
    Issue numberSUPPL. 3
    StatePublished - 1989

    Fingerprint

    Azo Compounds
    myc Genes
    Rats
    Genes
    Proto-Oncogene Proteins c-myc
    Hepatocytes
    Liver Extracts
    Oncogene Proteins
    Radioimmunoassay
    Proteins
    Peptides
    Antibodies
    Neoplasms

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)

    Cite this

    Suzuki, T., Yamaguchi, K., Abe, K., Yanaihara, C., Greeley, G. H., Mochizuki, T., ... Yanaihara, N. (1989). c-myc gene product in rat azo-dye-induced hepatocarcinogenesis. Biomedical Research, 10(SUPPL. 3), 577-586.

    c-myc gene product in rat azo-dye-induced hepatocarcinogenesis. / Suzuki, T.; Yamaguchi, K.; Abe, K.; Yanaihara, C.; Greeley, G. H.; Mochizuki, T.; Iguchi, K.; Hoshino, M.; Yanaihara, N.

    In: Biomedical Research, Vol. 10, No. SUPPL. 3, 1989, p. 577-586.

    Research output: Contribution to journalArticle

    Suzuki, T, Yamaguchi, K, Abe, K, Yanaihara, C, Greeley, GH, Mochizuki, T, Iguchi, K, Hoshino, M & Yanaihara, N 1989, 'c-myc gene product in rat azo-dye-induced hepatocarcinogenesis', Biomedical Research, vol. 10, no. SUPPL. 3, pp. 577-586.
    Suzuki T, Yamaguchi K, Abe K, Yanaihara C, Greeley GH, Mochizuki T et al. c-myc gene product in rat azo-dye-induced hepatocarcinogenesis. Biomedical Research. 1989;10(SUPPL. 3):577-586.
    Suzuki, T. ; Yamaguchi, K. ; Abe, K. ; Yanaihara, C. ; Greeley, G. H. ; Mochizuki, T. ; Iguchi, K. ; Hoshino, M. ; Yanaihara, N. / c-myc gene product in rat azo-dye-induced hepatocarcinogenesis. In: Biomedical Research. 1989 ; Vol. 10, No. SUPPL. 3. pp. 577-586.
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