c-myc gene product in rat azo-dye-induced hepatocarcinogenesis

T. Suzuki, K. Yamaguchi, K. Abe, C. Yanaihara, G. H. Greeley, T. Mochizuki, K. Iguchi, M. Hoshino, N. Yanaihara

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Abstract

Changes of c-myc gene-product levels were examined in liver extracts of rats having 3'-methyl-4-diaminoazobenzene-induced rat hepatocarcinogenesis, using an antibodies directed against a synthetic pentadecapeptide corresponding to the C-terminal 423-437 sequence of the predicted c-myc oncogene protein. After a 4 weeks treatment with 3'-methyl-4-diamino azobenzene (3'-Me-DAB), c-myc oncogene product-like immunoreactivity (c-myc-P-IR) was significantly increased as demonstrated by radioimmunoassay of rat liver extracts. In addition, immunohistochemical results of surviving and regenerating hepatocytes of 3 weeks and thereafter of 3-DAB-treated rats supported observations obtained by the radioimmunoassay. Hepatocytes of hyperplastic foci or neoplastic nodules were immunostained at about 80% in frequency. Foci of atypical hepatocellular proliferation less than 1 mm in diameter, which appeared from the 21st week after the start of treatment, harbored no or if present, a few immunoreactive cells, at 2.6% in frequency. Larger foci of atypical hepatocytes including cancer nodules had few or no immunoreactive cells. The present immunochemical approach which makes use of a synthetic peptide related to a functional protein (i.e. c-myc protein) is a worthwhile technique for detection of a wide range of functional proteins.

Original languageEnglish (US)
Pages (from-to)577-586
Number of pages10
JournalBiomedical Research
Volume10
Issue numberSUPPL. 3
StatePublished - 1989
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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    Suzuki, T., Yamaguchi, K., Abe, K., Yanaihara, C., Greeley, G. H., Mochizuki, T., Iguchi, K., Hoshino, M., & Yanaihara, N. (1989). c-myc gene product in rat azo-dye-induced hepatocarcinogenesis. Biomedical Research, 10(SUPPL. 3), 577-586.