C57BL/6 neuromuscular healthspan scoring system

Theodore Graber, Lisa Ferguson-Stegall, Jong Hee Kim, LaDora V. Thompson

Research output: Contribution to journalReview article

32 Citations (Scopus)

Abstract

Developing a scoring system based on physiological and functional measurements is critical to test the efficacy of potential interventions for sarcopenia and frailty in aging animal models; therefore, the aim of this study was to develop a neuromuscular healthspan scoring system (NMHSS). We examined three ages of male C57BL/6 mice: adults (6-7 months old, 100% survival), old (24-26 months old, 75% survival), and elderly group (>28 months old, ≤50% survival) - as well as mice along this age continuum. Functional performance (as determined by the rotarod and inverted-cling grip test) and in vitro muscle contractility were the determinants. A raw score was derived for each determinant, and the NMHSS was then derived as the sum of the individual determinant scores. In comparison with individual determinants, the NMHSS reduced the effect of individual variability within age groups, thus potentially providing an enhanced ability to detect treatment effects in future studies.

Original languageEnglish (US)
Pages (from-to)1326-1336
Number of pages11
JournalJournals of Gerontology - Series A Biological Sciences and Medical Sciences
Volume68
Issue number11
DOIs
StatePublished - Nov 2013
Externally publishedYes

Fingerprint

Survival
Sarcopenia
Hand Strength
Inbred C57BL Mouse
Animal Models
Age Groups
Muscles
Therapeutics
In Vitro Techniques

Keywords

  • Function
  • Healthspan
  • Muscle
  • Neuromuscular
  • Physiology

ASJC Scopus subject areas

  • Aging
  • Geriatrics and Gerontology
  • Medicine(all)

Cite this

C57BL/6 neuromuscular healthspan scoring system. / Graber, Theodore; Ferguson-Stegall, Lisa; Kim, Jong Hee; Thompson, LaDora V.

In: Journals of Gerontology - Series A Biological Sciences and Medical Sciences, Vol. 68, No. 11, 11.2013, p. 1326-1336.

Research output: Contribution to journalReview article

Graber, Theodore ; Ferguson-Stegall, Lisa ; Kim, Jong Hee ; Thompson, LaDora V. / C57BL/6 neuromuscular healthspan scoring system. In: Journals of Gerontology - Series A Biological Sciences and Medical Sciences. 2013 ; Vol. 68, No. 11. pp. 1326-1336.
@article{f9443777db7c474d83e412f0c7ce3d73,
title = "C57BL/6 neuromuscular healthspan scoring system",
abstract = "Developing a scoring system based on physiological and functional measurements is critical to test the efficacy of potential interventions for sarcopenia and frailty in aging animal models; therefore, the aim of this study was to develop a neuromuscular healthspan scoring system (NMHSS). We examined three ages of male C57BL/6 mice: adults (6-7 months old, 100{\%} survival), old (24-26 months old, 75{\%} survival), and elderly group (>28 months old, ≤50{\%} survival) - as well as mice along this age continuum. Functional performance (as determined by the rotarod and inverted-cling grip test) and in vitro muscle contractility were the determinants. A raw score was derived for each determinant, and the NMHSS was then derived as the sum of the individual determinant scores. In comparison with individual determinants, the NMHSS reduced the effect of individual variability within age groups, thus potentially providing an enhanced ability to detect treatment effects in future studies.",
keywords = "Function, Healthspan, Muscle, Neuromuscular, Physiology",
author = "Theodore Graber and Lisa Ferguson-Stegall and Kim, {Jong Hee} and Thompson, {LaDora V.}",
year = "2013",
month = "11",
doi = "10.1093/gerona/glt032",
language = "English (US)",
volume = "68",
pages = "1326--1336",
journal = "Journals of Gerontology - Series A Biological Sciences and Medical Sciences",
issn = "1079-5006",
publisher = "Oxford University Press",
number = "11",

}

TY - JOUR

T1 - C57BL/6 neuromuscular healthspan scoring system

AU - Graber, Theodore

AU - Ferguson-Stegall, Lisa

AU - Kim, Jong Hee

AU - Thompson, LaDora V.

PY - 2013/11

Y1 - 2013/11

N2 - Developing a scoring system based on physiological and functional measurements is critical to test the efficacy of potential interventions for sarcopenia and frailty in aging animal models; therefore, the aim of this study was to develop a neuromuscular healthspan scoring system (NMHSS). We examined three ages of male C57BL/6 mice: adults (6-7 months old, 100% survival), old (24-26 months old, 75% survival), and elderly group (>28 months old, ≤50% survival) - as well as mice along this age continuum. Functional performance (as determined by the rotarod and inverted-cling grip test) and in vitro muscle contractility were the determinants. A raw score was derived for each determinant, and the NMHSS was then derived as the sum of the individual determinant scores. In comparison with individual determinants, the NMHSS reduced the effect of individual variability within age groups, thus potentially providing an enhanced ability to detect treatment effects in future studies.

AB - Developing a scoring system based on physiological and functional measurements is critical to test the efficacy of potential interventions for sarcopenia and frailty in aging animal models; therefore, the aim of this study was to develop a neuromuscular healthspan scoring system (NMHSS). We examined three ages of male C57BL/6 mice: adults (6-7 months old, 100% survival), old (24-26 months old, 75% survival), and elderly group (>28 months old, ≤50% survival) - as well as mice along this age continuum. Functional performance (as determined by the rotarod and inverted-cling grip test) and in vitro muscle contractility were the determinants. A raw score was derived for each determinant, and the NMHSS was then derived as the sum of the individual determinant scores. In comparison with individual determinants, the NMHSS reduced the effect of individual variability within age groups, thus potentially providing an enhanced ability to detect treatment effects in future studies.

KW - Function

KW - Healthspan

KW - Muscle

KW - Neuromuscular

KW - Physiology

UR - http://www.scopus.com/inward/record.url?scp=84885980568&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84885980568&partnerID=8YFLogxK

U2 - 10.1093/gerona/glt032

DO - 10.1093/gerona/glt032

M3 - Review article

VL - 68

SP - 1326

EP - 1336

JO - Journals of Gerontology - Series A Biological Sciences and Medical Sciences

JF - Journals of Gerontology - Series A Biological Sciences and Medical Sciences

SN - 1079-5006

IS - 11

ER -