Caffeinated coffee blunts the myocardial protective effects of statins against ischemia-reperfusion injury in the rat

Yumei Ye, Ghassan H. Abu Said, Yu Lin, Saraswathy Manickavasagam, Michael G. Hughes, David J. McAdoo, Regino J. Perez-Polo, Yochai Birnbaum

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Purpose: We asked whether caffeinated coffee (CC) blunts the infarct size (IS)-limiting effects of atorvastatin (ATV). Background: Adenosine receptor activation is essential for mediating the IS-limiting effects of statins. Caffeine is a nonspecific adenosine receptor blocker, and thus drinking CC may block the myocardial protective effects of statins. Methods: Rat received 3-day ATV (10 mg/kg/day) or water by oral gavage once daily. Drinking water was replaced by water + sugar (7.5 g/100 ml), CC with sugar, or decaffeinated coffee (DC) with sugar. On the 4th day, rats were anesthetized and underwent 30 min of coronary artery occlusion and 4 h reperfusion. Area at risk was assessed by blue dye and infarct size by TTC. Results: Body weight and area at risk was comparable among groups. IS was 25.1±3.9% of the area at risk in the control group. In rats not receiving ATV, CC (25.5±3.1%) and DC (34.0±2.8%) did not affect IS. IS was significantly reduced by ATV in the water + sugar (11.7±0.7%, p=0.015) and DC (11.5±1.0%; p<0.001) groups, but not in the CC group (32.3±3.0%; p=0.719). ATV increased myocardial levels of Ser-473 phosphorylated Akt in the water + sugar and DC groups, but not in the CC group. Conclusions: CC, but not DC, abrogated the IS-limiting effects of ATV by blocking the adenosine receptors and preventing the phosphorylation of Akt. CC did not affect IS in rats not receiving ATV.

Original languageEnglish (US)
Pages (from-to)275-282
Number of pages8
JournalCardiovascular Drugs and Therapy
Volume22
Issue number4
DOIs
StatePublished - Aug 2008

Fingerprint

Hydroxymethylglutaryl-CoA Reductase Inhibitors
Coffee
Reperfusion Injury
Purinergic P1 Receptors
Water
Coronary Occlusion
Atorvastatin Calcium
Caffeine
Drinking Water
Drinking
Reperfusion

Keywords

  • Adenosine
  • Atorvastatin
  • Caffeine
  • Coffee
  • Infarct size

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Cardiology and Cardiovascular Medicine
  • Pharmacology

Cite this

Caffeinated coffee blunts the myocardial protective effects of statins against ischemia-reperfusion injury in the rat. / Ye, Yumei; Abu Said, Ghassan H.; Lin, Yu; Manickavasagam, Saraswathy; Hughes, Michael G.; McAdoo, David J.; Perez-Polo, Regino J.; Birnbaum, Yochai.

In: Cardiovascular Drugs and Therapy, Vol. 22, No. 4, 08.2008, p. 275-282.

Research output: Contribution to journalArticle

Ye, Y, Abu Said, GH, Lin, Y, Manickavasagam, S, Hughes, MG, McAdoo, DJ, Perez-Polo, RJ & Birnbaum, Y 2008, 'Caffeinated coffee blunts the myocardial protective effects of statins against ischemia-reperfusion injury in the rat', Cardiovascular Drugs and Therapy, vol. 22, no. 4, pp. 275-282. https://doi.org/10.1007/s10557-008-6105-z
Ye, Yumei ; Abu Said, Ghassan H. ; Lin, Yu ; Manickavasagam, Saraswathy ; Hughes, Michael G. ; McAdoo, David J. ; Perez-Polo, Regino J. ; Birnbaum, Yochai. / Caffeinated coffee blunts the myocardial protective effects of statins against ischemia-reperfusion injury in the rat. In: Cardiovascular Drugs and Therapy. 2008 ; Vol. 22, No. 4. pp. 275-282.
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abstract = "Purpose: We asked whether caffeinated coffee (CC) blunts the infarct size (IS)-limiting effects of atorvastatin (ATV). Background: Adenosine receptor activation is essential for mediating the IS-limiting effects of statins. Caffeine is a nonspecific adenosine receptor blocker, and thus drinking CC may block the myocardial protective effects of statins. Methods: Rat received 3-day ATV (10 mg/kg/day) or water by oral gavage once daily. Drinking water was replaced by water + sugar (7.5 g/100 ml), CC with sugar, or decaffeinated coffee (DC) with sugar. On the 4th day, rats were anesthetized and underwent 30 min of coronary artery occlusion and 4 h reperfusion. Area at risk was assessed by blue dye and infarct size by TTC. Results: Body weight and area at risk was comparable among groups. IS was 25.1±3.9{\%} of the area at risk in the control group. In rats not receiving ATV, CC (25.5±3.1{\%}) and DC (34.0±2.8{\%}) did not affect IS. IS was significantly reduced by ATV in the water + sugar (11.7±0.7{\%}, p=0.015) and DC (11.5±1.0{\%}; p<0.001) groups, but not in the CC group (32.3±3.0{\%}; p=0.719). ATV increased myocardial levels of Ser-473 phosphorylated Akt in the water + sugar and DC groups, but not in the CC group. Conclusions: CC, but not DC, abrogated the IS-limiting effects of ATV by blocking the adenosine receptors and preventing the phosphorylation of Akt. CC did not affect IS in rats not receiving ATV.",
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T1 - Caffeinated coffee blunts the myocardial protective effects of statins against ischemia-reperfusion injury in the rat

AU - Ye, Yumei

AU - Abu Said, Ghassan H.

AU - Lin, Yu

AU - Manickavasagam, Saraswathy

AU - Hughes, Michael G.

AU - McAdoo, David J.

AU - Perez-Polo, Regino J.

AU - Birnbaum, Yochai

PY - 2008/8

Y1 - 2008/8

N2 - Purpose: We asked whether caffeinated coffee (CC) blunts the infarct size (IS)-limiting effects of atorvastatin (ATV). Background: Adenosine receptor activation is essential for mediating the IS-limiting effects of statins. Caffeine is a nonspecific adenosine receptor blocker, and thus drinking CC may block the myocardial protective effects of statins. Methods: Rat received 3-day ATV (10 mg/kg/day) or water by oral gavage once daily. Drinking water was replaced by water + sugar (7.5 g/100 ml), CC with sugar, or decaffeinated coffee (DC) with sugar. On the 4th day, rats were anesthetized and underwent 30 min of coronary artery occlusion and 4 h reperfusion. Area at risk was assessed by blue dye and infarct size by TTC. Results: Body weight and area at risk was comparable among groups. IS was 25.1±3.9% of the area at risk in the control group. In rats not receiving ATV, CC (25.5±3.1%) and DC (34.0±2.8%) did not affect IS. IS was significantly reduced by ATV in the water + sugar (11.7±0.7%, p=0.015) and DC (11.5±1.0%; p<0.001) groups, but not in the CC group (32.3±3.0%; p=0.719). ATV increased myocardial levels of Ser-473 phosphorylated Akt in the water + sugar and DC groups, but not in the CC group. Conclusions: CC, but not DC, abrogated the IS-limiting effects of ATV by blocking the adenosine receptors and preventing the phosphorylation of Akt. CC did not affect IS in rats not receiving ATV.

AB - Purpose: We asked whether caffeinated coffee (CC) blunts the infarct size (IS)-limiting effects of atorvastatin (ATV). Background: Adenosine receptor activation is essential for mediating the IS-limiting effects of statins. Caffeine is a nonspecific adenosine receptor blocker, and thus drinking CC may block the myocardial protective effects of statins. Methods: Rat received 3-day ATV (10 mg/kg/day) or water by oral gavage once daily. Drinking water was replaced by water + sugar (7.5 g/100 ml), CC with sugar, or decaffeinated coffee (DC) with sugar. On the 4th day, rats were anesthetized and underwent 30 min of coronary artery occlusion and 4 h reperfusion. Area at risk was assessed by blue dye and infarct size by TTC. Results: Body weight and area at risk was comparable among groups. IS was 25.1±3.9% of the area at risk in the control group. In rats not receiving ATV, CC (25.5±3.1%) and DC (34.0±2.8%) did not affect IS. IS was significantly reduced by ATV in the water + sugar (11.7±0.7%, p=0.015) and DC (11.5±1.0%; p<0.001) groups, but not in the CC group (32.3±3.0%; p=0.719). ATV increased myocardial levels of Ser-473 phosphorylated Akt in the water + sugar and DC groups, but not in the CC group. Conclusions: CC, but not DC, abrogated the IS-limiting effects of ATV by blocking the adenosine receptors and preventing the phosphorylation of Akt. CC did not affect IS in rats not receiving ATV.

KW - Adenosine

KW - Atorvastatin

KW - Caffeine

KW - Coffee

KW - Infarct size

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