Calcitonin gene-related peptide stimulates human villous trophoblast cell differentiation in vitro

K. E. Green, C. Thota, Gary Hankins, C. Yallampalli, Yuan Lin Dong

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Calcitonin gene-related peptide (CGRP) is a multifunctional peptide present in both maternal and fetal circulations in pregnancy. Its receptors have been identified on human trophoblast cells, but the role of CGRP in trophoblast differentiation remains unknown. This study was designed to determine the effect of CGRP on the differentiation of villous trophoblasts isolated from normal human term placentae. The morphological and functional differentiation of the trophoblast cells were assessed by desmosomal protein immunofluorescent staining and the quantification of hCG, estrogen and progesterone secretion. Results showed that (i) exposure of villous trophoblast cells to CGRP led to a dose-dependent increase in intracellular cyclic adenosine monophosphate (cAMP) accumulation; (ii) immunofluorescent staining with antidesmosomal antibody was identified at the boundaries between aggregated cytotrophoblast cells, and these stainings disappeared when cells fused to form syncytiotrophoblast cells; (iii) the formation of multinucleated syncytiums in primary cultured cytotrophoblasts was stimulated by CGRP as evidenced by the changes in antidesmosomal staining; (iv) CGRP increases trophoblast hCG secretion in a time- and dose-dependent manner, and this secretion was blocked by CGRP antagonist, CGRP 8-37, and (v) both 17β-estradiol (E2) and progesterone concentrations in the culture medium were increased by CGRP, and these increases were dose dependent. These observations suggest that CGRP may be involved in the morphological and functional differentiation of trophoblast cells, and these actions might be attributed to CGRP-induced intracellular cAMP accumulation.

Original languageEnglish (US)
Pages (from-to)443-450
Number of pages8
JournalMolecular Human Reproduction
Volume12
Issue number7
DOIs
StatePublished - Jul 2006

Fingerprint

Calcitonin Gene-Related Peptide
Trophoblasts
Cell Differentiation
Staining and Labeling
Cyclic AMP
Progesterone
In Vitro Techniques
Placenta
Culture Media
Estradiol
Estrogens
Mothers
Pregnancy
Peptides
Antibodies

Keywords

  • CGRP
  • Placenta
  • Pregnancy
  • Trophoblasts

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Genetics
  • Developmental Biology
  • Embryology
  • Cell Biology

Cite this

Calcitonin gene-related peptide stimulates human villous trophoblast cell differentiation in vitro. / Green, K. E.; Thota, C.; Hankins, Gary; Yallampalli, C.; Dong, Yuan Lin.

In: Molecular Human Reproduction, Vol. 12, No. 7, 07.2006, p. 443-450.

Research output: Contribution to journalArticle

Green, K. E. ; Thota, C. ; Hankins, Gary ; Yallampalli, C. ; Dong, Yuan Lin. / Calcitonin gene-related peptide stimulates human villous trophoblast cell differentiation in vitro. In: Molecular Human Reproduction. 2006 ; Vol. 12, No. 7. pp. 443-450.
@article{4622d8cf151e4f7c9fca0466f032219c,
title = "Calcitonin gene-related peptide stimulates human villous trophoblast cell differentiation in vitro",
abstract = "Calcitonin gene-related peptide (CGRP) is a multifunctional peptide present in both maternal and fetal circulations in pregnancy. Its receptors have been identified on human trophoblast cells, but the role of CGRP in trophoblast differentiation remains unknown. This study was designed to determine the effect of CGRP on the differentiation of villous trophoblasts isolated from normal human term placentae. The morphological and functional differentiation of the trophoblast cells were assessed by desmosomal protein immunofluorescent staining and the quantification of hCG, estrogen and progesterone secretion. Results showed that (i) exposure of villous trophoblast cells to CGRP led to a dose-dependent increase in intracellular cyclic adenosine monophosphate (cAMP) accumulation; (ii) immunofluorescent staining with antidesmosomal antibody was identified at the boundaries between aggregated cytotrophoblast cells, and these stainings disappeared when cells fused to form syncytiotrophoblast cells; (iii) the formation of multinucleated syncytiums in primary cultured cytotrophoblasts was stimulated by CGRP as evidenced by the changes in antidesmosomal staining; (iv) CGRP increases trophoblast hCG secretion in a time- and dose-dependent manner, and this secretion was blocked by CGRP antagonist, CGRP 8-37, and (v) both 17β-estradiol (E2) and progesterone concentrations in the culture medium were increased by CGRP, and these increases were dose dependent. These observations suggest that CGRP may be involved in the morphological and functional differentiation of trophoblast cells, and these actions might be attributed to CGRP-induced intracellular cAMP accumulation.",
keywords = "CGRP, Placenta, Pregnancy, Trophoblasts",
author = "Green, {K. E.} and C. Thota and Gary Hankins and C. Yallampalli and Dong, {Yuan Lin}",
year = "2006",
month = "7",
doi = "10.1093/molehr/gal053",
language = "English (US)",
volume = "12",
pages = "443--450",
journal = "Molecular Human Reproduction",
issn = "1360-9947",
publisher = "Oxford University Press",
number = "7",

}

TY - JOUR

T1 - Calcitonin gene-related peptide stimulates human villous trophoblast cell differentiation in vitro

AU - Green, K. E.

AU - Thota, C.

AU - Hankins, Gary

AU - Yallampalli, C.

AU - Dong, Yuan Lin

PY - 2006/7

Y1 - 2006/7

N2 - Calcitonin gene-related peptide (CGRP) is a multifunctional peptide present in both maternal and fetal circulations in pregnancy. Its receptors have been identified on human trophoblast cells, but the role of CGRP in trophoblast differentiation remains unknown. This study was designed to determine the effect of CGRP on the differentiation of villous trophoblasts isolated from normal human term placentae. The morphological and functional differentiation of the trophoblast cells were assessed by desmosomal protein immunofluorescent staining and the quantification of hCG, estrogen and progesterone secretion. Results showed that (i) exposure of villous trophoblast cells to CGRP led to a dose-dependent increase in intracellular cyclic adenosine monophosphate (cAMP) accumulation; (ii) immunofluorescent staining with antidesmosomal antibody was identified at the boundaries between aggregated cytotrophoblast cells, and these stainings disappeared when cells fused to form syncytiotrophoblast cells; (iii) the formation of multinucleated syncytiums in primary cultured cytotrophoblasts was stimulated by CGRP as evidenced by the changes in antidesmosomal staining; (iv) CGRP increases trophoblast hCG secretion in a time- and dose-dependent manner, and this secretion was blocked by CGRP antagonist, CGRP 8-37, and (v) both 17β-estradiol (E2) and progesterone concentrations in the culture medium were increased by CGRP, and these increases were dose dependent. These observations suggest that CGRP may be involved in the morphological and functional differentiation of trophoblast cells, and these actions might be attributed to CGRP-induced intracellular cAMP accumulation.

AB - Calcitonin gene-related peptide (CGRP) is a multifunctional peptide present in both maternal and fetal circulations in pregnancy. Its receptors have been identified on human trophoblast cells, but the role of CGRP in trophoblast differentiation remains unknown. This study was designed to determine the effect of CGRP on the differentiation of villous trophoblasts isolated from normal human term placentae. The morphological and functional differentiation of the trophoblast cells were assessed by desmosomal protein immunofluorescent staining and the quantification of hCG, estrogen and progesterone secretion. Results showed that (i) exposure of villous trophoblast cells to CGRP led to a dose-dependent increase in intracellular cyclic adenosine monophosphate (cAMP) accumulation; (ii) immunofluorescent staining with antidesmosomal antibody was identified at the boundaries between aggregated cytotrophoblast cells, and these stainings disappeared when cells fused to form syncytiotrophoblast cells; (iii) the formation of multinucleated syncytiums in primary cultured cytotrophoblasts was stimulated by CGRP as evidenced by the changes in antidesmosomal staining; (iv) CGRP increases trophoblast hCG secretion in a time- and dose-dependent manner, and this secretion was blocked by CGRP antagonist, CGRP 8-37, and (v) both 17β-estradiol (E2) and progesterone concentrations in the culture medium were increased by CGRP, and these increases were dose dependent. These observations suggest that CGRP may be involved in the morphological and functional differentiation of trophoblast cells, and these actions might be attributed to CGRP-induced intracellular cAMP accumulation.

KW - CGRP

KW - Placenta

KW - Pregnancy

KW - Trophoblasts

UR - http://www.scopus.com/inward/record.url?scp=33747878306&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33747878306&partnerID=8YFLogxK

U2 - 10.1093/molehr/gal053

DO - 10.1093/molehr/gal053

M3 - Article

C2 - 16772432

AN - SCOPUS:33747878306

VL - 12

SP - 443

EP - 450

JO - Molecular Human Reproduction

JF - Molecular Human Reproduction

SN - 1360-9947

IS - 7

ER -